symbiont-mediated rna interference in insects. | rna interference (rnai) methods for insects are often limited by problems with double-stranded (ds) rna delivery, which restricts reverse genetics studies and the development of rnai-based biocides. we therefore delegated to insect symbiotic bacteria the task of: (i) constitutive dsrna synthesis and (ii) trauma-free delivery. rnaseiii-deficient, dsrna-expressing bacterial strains were created from the symbionts of two very diverse pest species: a long-lived blood-sucking bug, rhodnius prolixus, ... | 2016 | 26911963 |