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methods for rapid and effective pcr-based detection of 'candidatus liberibacter solanacearum' from the insect vector bactericera cockerelli: streamlining the dna extraction/purification process.this study provides a protocol for rapid dna isolation from psyllid vectors (bactericera cockerelli and diaphorina citri) that can be used directly with dna-based methods for the detection of 'candidatus (ca.) liberibacter solanacearum,' the bacterial causal agent of potato zebra chip disease and eventually for 'ca. liberibacter asiaticus' the causal agent of huanglongbing disease in citrus. the fast dna extraction protocol was designed to work with conventional polymerase chain reaction (cpcr) ...201323865212
comparison of potato and asian citrus psyllid adult and nymph transcriptomes identified vector transcripts with potential involvement in circulative, propagative liberibacter transmission.the potato psyllid (pop) bactericera cockerelli (sulc) and asian citrus psyllid (acp) diaphorina citri kuwayama are the insect vectors of the fastidious plant pathogen, candidatus liberibacter solanacearum (clso) and ca. l. asiaticus (clas), respectively. clso causes zebra chip disease of potato and vein-greening in solanaceous species, whereas, clas causes citrus greening disease. the reliance on insecticides for vector management to reduce pathogen transmission has increased interest in altern ...201425436509
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