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rna1-independent replication and gfp expression from tomato marchitez virus isolate m cloned cdna.tomato marchitez virus (tomarv; synonymous with tomato apex necrosis virus) is a positive-strand rna virus in the genus torradovirus within the family secoviridae. tomarv is an emergent whitefly-transmitted virus that causes important diseases in tomato (solanum lycopersicum) in mexico. here, the genome sequence of the tomarv isolate m (tomarv-m) was determined. we engineered full-length cdna clones of the tomarv-m genomic rna (rna1 and rna2), separately, into a binary vector. coinfiltration of ...201626756828
torradoviruses are transmitted in a semi-persistent and stylet-borne manner by three whitefly vectors.members of the genus torradovirus (family secoviridae, type species tomato torrado virus, totv) are spherical plant viruses transmitted by the whitefly species trialeurodes vaporariorum and bemisia tabaci. knowledge on the mode of vector transmission is lacking for torradoviruses. here, the mode of transmission was determined for tomato marchitez virus (tomarv). a minimal acquisition access period (aap) and inoculation access period (iap) of approximately 2h each was required for its transmissio ...201424342139
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