| whitefly transmission and efficient ssdna accumulation of bean golden mosaic geminivirus require functional coat protein. | bean golden mosaic geminivirus (bgmv) has a bipartite genome composed of two circular ssdna components (dna-a and dna-b) and is transmitted by the whitefly, bemisia tabaci. dna-a encodes the viral replication proteins and the coat protein. to determine the role of bgmv coat protein systemic infection and whitefly transmission, two deletions and a restriction fragment inversion were introduced into the bgmv coat protein gene. all three coat protein mutants produced systemic infections when coinoc ... | 1994 | 8091659 |
| rnai-mediated resistance to bean golden mosaic virus in genetically engineered common bean (phaseolus vulgaris). | bean golden mosaic virus (bgmv) is transmitted by the whitefly bemisia tabaci in a persistent, circulative manner, causing the golden mosaic of common bean (phaseolus vulgaris l.). the characteristic symptoms are yellow-green mosaic of leaves, stunted growth, or distorted pods. the disease is the largest constraint to bean production in latin america and causes severe yield losses (40 to 100%). here, we explored the concept of using an rna interference construct to silence the sequence region of ... | 2007 | 17555279 |
| [biological aspects of bemisia tabaci (genn.) b biotype (hemiptera: aleyrodidae) on six bean genotypes]. | the silverleaf whitefly is one of the most harmful pests that attack bean crops, mainly for extracting large quantities of phloem sap and transmitting the bean golden mosaic virus. resistant germoplasm plants can be an important method for controlling this pest. the biological aspects of bemisia tabaci b biotype on bean (phaseolus vulgaris) genotypes were evaluated. the tests were conducted under laboratory conditions, with the following genotypes: arc 1, arc 3s, arc 5s, g13028, g11056 and porri ... | 2008 | 18506300 |
| biological and molecular properties of a begomovirus from dicliptera sexangularis. | abstract sixangle foldwing, dicliptera sexangularis (acanthaceae), showing severe yellow mottle and leaf distortion symptoms was collected from the shoreline of calusa island (lee county, fl). the putative virus was transmitted from infected d. sexangularis to healthy seedlings by mechanical, whitefly (bemisia tabaci biotype b), and graft-inoculations. different forms of geminivirus-like dnas were detected in total dna extracted from infected plants by southern blot hybridization analyses using ... | 2000 | 18944491 |
| reduction of viral load in whitefly (bemisia tabaci gen.) feeding on rnai-mediated bean golden mosaic virus resistant transgenic bean plants. | the rnai concept was explored to silence the rep gene from the bean golden mosaic virus (bgmv) and a genetically modified (gm) bean immune to the virus was previously generated. we investigated if bgmv-viruliferous whiteflies would reduce viral amount after feeding on gm plants. bgmv dna amount was significantly reduced in whiteflies feeding in gm-plants (compared with insects feeding on non-gm plants) for a period of 4 and 8 days in 52% and 84% respectively. | 2015 | 26297125 |