| detection of stylet-borne and circulative potato viruses in aphids by duplex reverse transcription polymerase chain reaction. | a reverse transcription polymerase chain reaction (rt-pcr) assay was designed to amplify stylet-borne potato virus yo (pvyo) in aphids using primers located in the viral capsid gene. a 480 bp long product was detected in aphids exposed to pvyo-infected potato plants. approximately 40% of myzus persicae and 15% of aphis nasturtii exposed briefly to pvyo-infected plants acquired the virus. this rate of acquisition by both species of aphids was typical of our earlier observation of the virus transm ... | 1996 | 8793847 |
| cytochrome c oxidase mrna as an internal control for detection of potato virus y and potato leafroll virus from single aphids by a co-amplification rt-pcr assay. | using cytochrome c oxidase subunit 1 (cox1) mrna as the internal control, a triplex reverse transcription-polymerase chain reaction (rt-pcr) for detection of potato virus y (pvy) and potato leafroll virus (plrv) with co-amplification of cox1 from single specimens of various aphid species has been developed. partial length cdna of cox1 from green peach aphid, myzus persicae (sulzer), potato aphid, macrosiphum euphorbiae (thomas), buckthorn aphid, aphis nasturtii (kaltenbach), and pea aphid, acyrt ... | 2006 | 17010449 |
| effect of mixed viral infections (potato virus y-potato leafroll virus) on biology and preference of vectors myzus persicae and macrosiphum euphorbiae (hemiptera: aphididae). | mixed viral infections of heterologous viruses such as potato virus y (family potyviridae, genus potyvirus, pvy) and potato leafroll virus (family luteoviridae, genus polerovirus, plrv) are a regular occurrence in idaho's potato, solanum tuberosum (l.), cropping systems. an increased number of plant samples from idaho's potato fields over the past 2 yr has serologically tested positive for both pvy and plrv via double antibody sandwich enzyme-linked immunosorbent assay (das-elisa) and exhibited ... | 2007 | 17598521 |
| effect of an alternate weed host, hairy nightshade, solanum sarrachoides, on the biology of the two most important potato leafroll virus (luteoviridae: polerovirus) vectors, myzus persicae and macrosiphum euphorbiae (aphididae: homoptera). | hairy nightshade, solanum sarrachoides (sendtner), is a ubiquitous weed in potato agro-ecosystems and nonagricultural lands of southeastern idaho and the pacific northwest. this weed increases the complexity of the potato leafroll virus (plrv) (luteoviridae: polervirus)-potato pathosystem by serving as aphid and virus reservoir. previous field studies showed higher densities of green peach aphid, myzus persicae (sulzer), and potato aphid, macrosiphum euphorbiae (thomas), the two most important v ... | 2008 | 18419933 |