| synthesis of a full-length infectious cdna clone of cucurbit aphid-borne yellows virus and its use in gene exchange experiments with structural proteins from other luteoviruses. | a full-length cdna of cucurbit aphid-borne yellows virus (cabyv) has been constructed and expressed either as an in vitro transcript, under control of a bacteriophage t7 rna polymerase promoter, or in vivo, under control of the cauliflower mosaic virus 35s promoter in an agroinfection vector. the biological activity of the cloned cdna was demonstrated by the ability of its in vitro transcript to replicate in protoplasts and of the agroinfection vector to infect agroinoculated plants. virus in th ... | 1995 | 8525610 |
| posterior midgut and hindgut are both sites of acquisition of cucurbit aphid-borne yellows virus in myzus persicae and aphis gossypii. | members of the family luteoviridae ('luteovirids') rely strictly on aphid vectors for plant-to-plant transmission. this interaction operates according to a persistent and circulative manner, which implies that the virions are being endocytosed and exocytosed across two epithelial barriers (alimentary tract and accessory salivary glands) in the vector's body. in several luteovirid-aphid vector species combinations, the route of virions in the insect has been investigated ultrastructurally by tran ... | 2003 | 14645929 |
| complete sequence analysis reveals two distinct poleroviruses infecting cucurbits in china. | the complete rna genomes of a chinese isolate of cucurbit aphid-borne yellows virus (cabyv-chn) and a new polerovirus tentatively referred to as melon aphid-borne yellows virus (mabyv) were determined. the entire genome of cabyv-chn shared 89.0% nucleotide sequence identity with the french cabyv isolate. in contrast, nucleotide sequence identities between mabyv and cabyv and other poleroviruses were in the range of 50.7-74.2%, with amino acid sequence identities ranging from 24.8 to 82.9% for in ... | 2008 | 18414972 |
| distribution and molecular diversity of three cucurbit-infecting poleroviruses in china. | cucurbit aphid-borne yellows virus (cabyv) and melon aphid-borne yellows virus (mabyv) have been found to be associated with cucurbit yellowing disease in china. our report identifies for the first time a third distinct polerovirus, tentatively named suakwa aphid-borne yellows virus (sabyv), infecting suakwa vegetable sponge. to better understand the distribution and molecular diversity of these three poleroviruses infecting cucurbits, a total of 214 cucurbitaceous crop samples were collected fr ... | 2009 | 19664664 |
| biological and molecular characterization of a korean isolate of cucurbit aphid-borne yellows virus infecting cucumis species in korea. | surveys of yellowing viruses in plastic tunnels and in open field crops of melon (cucumis melo cultivar catalupo), oriental melon (c. melo cultivar oriental melon), and cucumber (c. sativus) were carried out in two melon-growing areas in 2014, korea. severe yellowing symptoms on older leaves of melon and chlorotic spots on younger leaves of melon were observed in the plastic tunnels. the symptoms were widespread and included initial chlorotic lesions followed by yellowing of whole leaves and thi ... | 2015 | 26673519 |
| control of insect vectors and plant viruses in protected crops by novel pyrethroid-treated nets. | long-lasting insecticide-treated nets (llitns) constitute a novel alternative that combines physical and chemical tactics to prevent insect access and the spread of insect-transmitted plant viruses in protected enclosures. this approach is based on a slow-release insecticide-treated net with large hole sizes that allow improved ventilation of greenhouses. the efficacy of a wide range of llitns was tested under laboratory conditions against myzus persicae, aphis gossypii and bemisia tabaci. two n ... | 2015 | 25404196 |
| spatio-temporal dynamics of viruses are differentially affected by parasitoids depending on the mode of transmission. | relationships between agents in multitrophic systems are complex and very specific. insect-transmitted plant viruses are completely dependent on the behaviour and distribution patterns of their vectors. the presence of natural enemies may directly affect aphid behaviour and spread of plant viruses, as the escape response of aphids might cause a potential risk for virus dispersal. the spatio-temporal dynamics of cucumber mosaic virus (cmv) and cucurbit aphid-borne yellows virus (cabyv), transmitt ... | 2012 | 23202516 |
| incidence and molecular diversity of poleroviruses infecting cucurbit crops and weed plants in thailand. | overall, 244 samples of cucurbit crops with yellowing symptoms and selected weed species, from 15 provinces in thailand, were screened by rt-pcr using primers polero-cp-f and polero-cp-r. a total of 160 samples (~66%) were infected by poleroviruses. analysis of a 1.4 kb region covering the 3' rna-dependent rna polymerase (rdrp) gene, the intergenic non-coding region (incr), and the coat protein (cp), showed that four poleroviruses, namely, cucurbit aphid-borne yellows virus (cabyv), luffa aphid- ... | 2017 | 28352973 |
| resistance to cucurbit aphid-borne yellows virus in melon accession tgr-1551. | the genetic control of resistance to cucurbit aphid-borne yellows virus (cabyv; genus polerovirus, family luteoviridae) in the tgr-1551 melon accession was studied through agroinoculation of a genetic family obtained from the cross between this accession and the susceptible spanish cultivar 'bola de oro'. segregation analyses were consistent with the hypothesis that one dominant gene and at least two more modifier genes confer resistance; one of these additional genes is likely present in the su ... | 2015 | 26075973 |
| genetic diversity and potential vectors and reservoirs of cucurbit aphid-borne yellows virus in southeastern spain. | the genetic variability of a cucurbit aphid-borne yellows virus (cabyv) (genus polerovirus, family luteoviridae) population was evaluated by determining the nucleotide sequences of two genomic regions of cabyv isolates collected in open-field melon and squash crops during three consecutive years in murcia (southeastern spain). a phylogenetic analysis showed the existence of two major clades. the sequences did not cluster according to host, year, or locality of collection, and nucleotide similari ... | 2013 | 23802870 |
| impact of vat resistance in melon on viral epidemics and genetic structure of virus populations. | cultivar choice is at the heart of cropping systems and resistant cultivars should be at the heart of disease management strategies whenever available. they are the easiest, most efficient and environmentally friendly way of combating viral diseases at the farm level. among the melon genetic resources, vat is a unique gene conferring resistance to both the melon aphid aphis gossypii and the viruses it carries. the 'virus side' of this pleiotropic phenotype is seldom regarded as an asset for viru ... | 2017 | 28587865 |