| dependence of groundnut rosette virus on its satellite rna as well as on groundnut rosette assistor luteovirus for transmission by aphis craccivora. | transmission of groundnut rosette virus (grv) by aphis craccivora is known to depend on the additional presence in the source plants of a luteovirus, groundnut rosette assistor virus (grav). naturally occurring isolates of grv contain a satellite rna which is the main cause of rosette symptoms in groundnut, different variants of the satellite being responsible for the green and chlorotic forms of rosette. in extensive glasshouse tests, grav-dependent transmission of grv by a. craccivora occurred ... | 1990 | 1698926 |
| detection of each of the causal agents of groundnut rosette disease in plants and vector aphids by rt-pcr. | detection of the three agents of groundnut rosette disease (groundnut rosette assistor virus, groundnut rosette virus and its satellite rna) in plants and vector aphids by reverse transcription-polymerase chain reaction (rt-pcr) is reported. three procedures for extraction of total rna from groundnut were tested, of which two were found to be useful in giving rna of sufficient quality for rt-pcr. of these two, the total rna extraction kit supplied by qiagen was found to be the most versatile for ... | 1998 | 9923735 |
| spatiotemporal separation of groundnut rosette disease agents. | abstract analysis by triple-antibody sandwich enzyme-linked immunosorbent assay of groundnut samples from fields in two seasons from different regions of malawi showed the absence of groundnut rosette assistor virus (grav) from some plants showing groundnut rosette disease symptoms and the presence of grav in some symptomless plants. viruliferous aphis craccivora collected from fields transmitted either grav alone, groundnut rosette virus (grv) with its satellite rna (sat rna), or all three agen ... | 1999 | 18944738 |
| chickpea chlorotic stunt virus: a new polerovirus infecting cool-season food legumes in ethiopia. | abstract serological analysis of diseased chickpea and faba bean plantings with yellowing and stunting symptoms suggested the occurrence of an unknown or uncommon member of the family luteoviridae in ethiopia. degenerate primers were used for reverse transcriptase-polymerase chain reaction amplification of the viral coat protein (cp) coding region from both chickpea and faba bean samples. cloning and sequencing of the amplicons yielded nearly identical (96%) nucleotide sequences of a previously ... | 2006 | 18944302 |