| investigations on the role of a lysozyme from the malaria vector anopheles dirus during malaria parasite development. | a cdna encoding a lysozyme was obtained by rapid amplification of cdna ends-polymerase chain reaction (race-pcr) from females of the malaria vector anopheles dirus a (diptera: culicidae). the 623bp lysozyme (adlys c-1) cdna encodes the 120 amino acid mature protein with a predicted molecular mass of 13.4kda and theoretical pi of 8.45. six cysteine residues and a potential calcium binding motif that are present in adlys c-1 are highly conserved relative to those of c-type lysozymes found in other ... | 2011 | 21741400 |
| the fibrinogen-like domain of frep1 protein is a broad-spectrum malaria transmission-blocking vaccine antigen. | frep1 in mosquito midguts facilitates plasmodium falciparum parasite transmission. the fibrinogen-like (fbg) domain of frep1 is highly conserved (>90% identical) among anopheles species from different continents, suggesting that anti-fbg antibodies may block malaria transmission to all anopheline mosquitoes. using standard membrane-feeding assays, anti-frep1 polyclonal antibodies significantly blocked transmission of plasmodium berghei and plasmodium vivax to anopheles gambiae and anopheles diru ... | 2017 | 28533429 |
| quantitative real-time pcr analysis of anopheles dirus tep1 and nos during plasmodium berghei infection, using three reference genes. | quantitative reverse transcription pcr (qrt-pcr) has been an integral part of characterizing the immunity of anopheles mosquitoes towards plasmodium invasion. two anti-plasmodium factors of anopheles, thioester-containing protein 1 (tep1) and nitric oxide synthase (nos), play a role in the refractoriness of anopheles towards plasmodium infection and are generally expressed during infection. however, these are less studied in anopheles dirus, a dominant malaria vector in southeast asia. furthermo ... | 2017 | 28761783 |