| growth of the phlebovirus toscana in a mosquito (aedes pseudoscutellaris) cell line (ap-61): establishment of a persistent infection. | toscana virus, a sandfly-associated phlebovirus, was adapted to grow in cultured aedes pseudoscutellaris (ap-61) mosquito cell line. no evidence of virus growth was seen after primary infection of cell monolayers under maintenance conditions. on the contrary, persistent infections were established by subculturing infected cultures. cytopathic effect was never observed. significant titres of virus (10(3)-10(5) pfu/ml), as assayed in vero cells at 37 degrees c, were released from persistent infect ... | 1985 | 4015410 |