isolation of rift valley fever virus from human peripheral blood mononuclear cells: mauritanian epidemic. | during the mauritanian rift valley fever (rvf) epidemic of 1987, peripheral blood mononuclear cells (pbmc) were studied from 78 sick patients. rvf virus (rvfv) was isolated in 5 cases, on aedes pseudoscutellaris ap61, from both pbmc and serum. among the 78 cases studied, rvf was proven in 19 cases (24.3%) by specific igm detection, and in 12 cases (15.3%) by virus isolation from serum, of which 3 also exhibited anti-rvf igm. among the 5 pbmc-positive rvfv cases, 2 strains were isolated in the pr ... | 1989 | 2667067 |
isolation of the rift valley fever virus by inoculation into aedes pseudoscutellaris cells: comparison with other diagnostic methods. | the rift valley fever epidemic, which arose in the south of mauritania beginning on october 15, 1987, enabled a comparative study of different diagnostic methods among humans. during the first two weeks of the epidemic, four parallel methods were used: inoculation into aedes pseudoscutellaris cells, inoculation intracerebrally into suckling mice, tests by immunocapture of the circulating antigen and detection of type igm gammaglobulins. of 370 examined sera, 181 showed at least one marker of rec ... | 1989 | 2711043 |