| the ultrastructures of dengue type 1 virus in the aedes pseudoscutellaris mosquito cell line. | | 1979 | 442167 |
| dengue in the caribbean: virus isolation in a mosquito (aedes pseudoscutellaris) cell line. | during outbreaks of dengue fever in the caribbean in 1977 and 1978 a continuous cell line derived from aedes pseudoscutellaris was successfully used for the isolation of dengue virus strains from acute human sera. 238 strains were isolated and culture fluid was successfully used as antigen for the identification of several strains. the isolates all produced a marked syncytial cytopathic effect often visible as early as four days after inoculation. the method was successfully employed in the fiel ... | 1979 | 442177 |
| dengue fever in american military personnel in the philippines: clinical observations on hospitalized patients during a 1984 epidemic. | from june - august, 1984, 24 american military personnel were hospitalized with dengue (den) at clark air base in the philippines. their infections were confirmed by serology using the hemagglutination-inhibition test and/or by virus isolation in aedes pseudoscutellaris cell cultures. most of the patients had a secondary type of antibody response probably reflecting prior vaccination against yellow fever. three serotypes of den virus were isolated; 7 isolates of den 1, 4 isolates of den 3 and 3 ... | 1989 | 2772694 |
| isolation of dengue 2 and dengue 4 viruses from patients in senegal. | dengue 2 and dengue 4 viruses were isolated and re-isolated by inoculation into aedes pseudoscutellaris continuous cell line (mos 61) and/or toxrhynchites brevipalpis. the strain of dengue 2 had been isolated from a patient returning from casamance (south-western senegal) and two strains of dengue 4 from patients who lived in dakar and had not been outside the town in the 15 days before becoming ill. serological evidence of dengue 4 infection was found in another patient living in casamance. | 1986 | 3726996 |
| factors affecting syncytial development in aedes pseudoscutellaris cells by dengue viruses. | several factors that were suspected of affecting the development of syncytia in cultured aedes pseudoscutellaris cells inoculated with dengue viruses were studied. the results indicated that fresh media (less than 1 week old), low cell density at inoculation (2.8 x 10(5) cells/cm2), and low cell passage level (less than 52 passages) favored the development of syncytia. all three types (1, 2, and 3) of dengue viruses tested could be isolated from human sera by aedes pseudoscutellaris cell culture ... | 1981 | 7258498 |
| aedes albopictus and other aedes (stegomyia) species in fiji. | during an assessment of the dengue situation in fiji in early 1992, a house-to-house survey of container-breeding aedes was made. discarded tires and water drums were identified as key breeding sites for the 4 potential dengue vectors: aedes aegypti, aedes albopictus, aedes pseudoscutellaris, and aedes polynesiensis. aedes albopictus were detected on viti levu, vanua levu, and on taveuni. examination of early records and of used tire importation suggests entry into fiji after july 1985 but well ... | 1995 | 7595450 |
| comparison of various methods of detection of different forms of dengue virus type 2 rna in cultured cells. | in this report, the sensitivity of various methods of detection of dengue virus type 2 (den-2) sense, antisense, replicative intermediate (ri) and replicative form (rf) rnas in infected mosquito aedes pseudoscutellaris ap-61 and mammalian baby hamster kidney bhk-21 cells is compared. licl precipitation was used for separation of viral rf rna from ri rna. our results show that reverse transcription-polymerase chain reaction (rt-pcr) followed by southern blot analysis and slot blot hybridisation o ... | 1997 | 9607089 |
| [preliminary study of ap-64 cell line (aedes pseudoscutellaris) for dengue-1 and -2 virus multiplication]. | we study a new arthropod cell line, ap-64, which was obtained from aedes pseudoscutellaris larvae. the best growth and maintenance media are defined, testing the adequacy of dengue 1 and 2 viruses which become manifest by the appearance of the cytopathogenic effect as syncytiums. the immunofluorescence technique is applied to detect early multiplication of those viruses. | 1991 | 9768180 |
| [obtaining a clone of the ap-61 cell line. its usefulness in the multiplication of dengue viruses 1 and 2]. | the obtention of a clone of the cell line ap-61 (aedes pseudoscutellaris) is reported. details of the cloning and culture media employed are discussed. usefulness of the clone for the multiplication of dengue 1 and 2 viruses is proved in comparison with the original line using the indirect immunofluorescence technique. | 1992 | 9768212 |
| [biological behavior of 3 strains of the dengue-2 virus in 2 cell lines of mosquitoes]. | strains a-15 (isolated in cuba, 1981), jamaica (isolated in jamaica, 1981) and nueva guinea "c" (standard) from dengue-2 virus were compared according to the time of appearance of the cytopathic effect (cpe), to the time of appearance of specific fluorescence and to the kynetics of viral multiplication on being innoculated in the cell lines ap-61 (aedes pseudoscutellaris) and c6/36 ht (aedes albopictus). the results showed that the cpe of highest intensity and earliest appearance was for a-15, f ... | 2000 | 11826526 |
| continuous cell lines and immune ascitic fluid pools in arbovirus detection. | successive experiments led us to use two cellular systems, mos61 (aedes pseudoscutellaris cells) and vero cells, among the continuous cell lines recommended by the who collaborating center for systematic research and isolation of arboviruses. virus detection in cell cultures is carried out with 7 mixtures containing 10 hyperimmune ascitic fluids made with the reference viruses. this technique enables the detection of 70 of the 80 arboviruses transmitted by mosquitoes in africa and very easily de ... | 2006 | 1297177 |