| spontaneous pasteurellosis in captive rocky mountain bighorn sheep (ovis canadensis canadensis): clinical, laboratory, and epizootiological observations. | we observed clinical signs, compared adrenal responses, and performed diagnostic tests on 12 captive rocky mountain bighorn sheep (ovis canadensis canadensis) during a spontaneous outbreak of pasteurellosis. cortisol in urine and feces was measured for bighorns sampled three times between 20 october and 1 november 1986. by 6 november, four of these had developed pneumonia, four showed only mild rhinitis, and four remained clinically normal. bighorns that ultimately developed pneumonia showed ele ... | 1991 | 1758018 |
| isolation of pasteurella haemolytica from tonsillar biopsies of rocky mountain bighorn sheep. | isolations of pasteurella haemolytica were compared from tonsillar biopsies versus nasal passages for 29 free-ranging rocky mountain bighorn sheep (ovis canadensis canadensis) from central idaho. overall, p. haemolytica was isolated from 11 (38%) of 29 sheep. two (18%) of the 11 positive samples were from only nasal passages compared to eight (73%) from tonsillar biopsies. pasteurella haemolytica biotype t was isolated from tonsils of nine sheep and from nasal biopsies. pasteurella haemolytica b ... | 1990 | 2338725 |
| experimental contact transmission of pasteurella haemolytica from clinically normal domestic sheep causing pneumonia in rocky mountain bighorn sheep. | two rocky mountain bighorn lambs (ovis canadensis canadensis) were held in captivity for 120 days before being housed with two domestic sheep. the lambs were clinically normal and had no pasteurella spp. on nasal swab cultures. the domestic sheep were known to carry pasteurella haemolytica biotype a in the nasal passages. after being in close contact for 19 days. p. haemolytica biotype a was cultured from nasal swabs of one of the bighorn lambs. by 26 days, both bighorn sheep developed coughs, w ... | 1988 | 3193561 |
| susceptibility of phagocytes from elk, deer, bighorn sheep, and domestic sheep to pasteurella haemolytica cytotoxins. | alveolar macrophages and peripheral blood neutrophils from elk (cervus elaphus), bighorn sheep (ovis canadensis canadensis), and domestic sheep were exposed to culture supernatants from pasteurella haemolytica isolated from bighorn sheep and domestic sheep. in a second experiment, peripheral blood neutrophils from mule deer (odocoileus hemionus), elk, and bighorn sheep were exposed to culture supernatants from p. haemolytica isolated from elk, bighorn sheep and domestic sheep. alveolar macrophag ... | 1994 | 7760482 |
| bacteria isolated from nasal and tonsillar samples of clinically healthy rocky mountain bighorn and domestic sheep. | nasal and tonsillar samples were collected from 14 free-ranging clinically healthy rocky mountain bighorn sheep (ovis canadensis canadensis) and 10 domestic sheep (ovis aries). we identified 194 bacterial isolates, including 101 from bighorn and 93 from domestic sheep. of these isolates, 115 were gram-positive and 79 were gram-negative. staphylococcus species were the most numerous gram-positive organisms and had a higher incidence in samples from domestic than from bighorn sheep. in contrast st ... | 1994 | 8151809 |
| effects of modified cary and blair medium on recovery of nonhemolytic pasteurella haemolytica from rocky mountain bighorn sheep (ovis canadensis canadensis) pharyngeal swabs. | modified cary and blair transport medium (mcb) was evaluated for recovery of pasteurella spp. from pharyngeal swabs of healthy rocky mountain bighorn sheep (ovis canadensis canadensis). in experiment one, three pharyngeal swabs were collected from each of 25 bighorns. pasteurella haemolytica was recovered from 21 of 25 swabs tested almost immediately and from 16 of 25 swabs held in mcb medium at about 22 c for 24 hr before testing (p > 0.10). recovery of p. haemolytica decreased (p < 0.005) to 1 ... | 1994 | 8151818 |
| pasteurella haemolytica cytotoxin-dependent killing of neutrophils from bighorn and domestic sheep. | peripheral blood neutrophils from rocky mountain bighorn sheep (ovis canadensis canadensis) and domestic sheep were exposed to culture supernatants from pasteurella haemolytica isolates recovered from these two sheep species. six culture supernatants from bighorn sheep isolates and two from domestic sheep isolates were tested for cytotoxicity as determined by the release of lactate dehydrogenase. two of the bacterial culture supernatants from bighorn sheep were not cytotoxic, while the other fou ... | 1993 | 8445788 |
| effect of simulated stress on susceptibility of bighorn sheep neutrophils to pasteurella haemolytica leukotoxin. | we examined the effects of simulated stress on susceptibility of rocky mountain bighorn sheep (ovis canadensis canadensis) neutrophils to pasteurella haemolytica leukotoxin in a blocked, crossover experiment. ten captive-raised bighorn sheep were sampled 10 hr after separate administrations of long-acting adrenocorticotrophic hormone (acth) gel and normal saline (control). we then compared in vitro leukotoxin-dependent neutrophil death rates after exposure to culture supernatants from four uniqu ... | 1997 | 9249702 |
| experimental contact of bighorn sheep (ovis canadensis) with horses and cattle, and comparison of neutrophil sensitivity to pasteurella haemolytica cytotoxins. | peripheral blood neutrophils from horses, cattle, and rocky mountain bighorn sheep (ovis canadensis canadensis) were evaluated for susceptibility to cytotoxin-dependent lysis of different biotypes and serotypes of pasteurella haemolytica of domestic sheep, cattle, bighorn sheep, or mountain goat (oreamnos americana) origin utilizing a cytotoxicity assay which measures the degree of bacteria cytotoxin-killing of neutrophils. all isolates of p. haemolytica (biotypes a and t) were noncytotoxic to h ... | 1996 | 9359057 |
| sharing of pasteurella spp. between free-ranging bighorn sheep and feral goats. | pasteurella spp. were isolated from feral goats and free-ranging bighorn sheep (ovis canadensis canadensis) in the hells canyon national recreation area bordering idaho, oregon, and washington (usa). biovariant 1 pasteurella haemolytica organisms were isolated from one goat and one of two bighorn sheep found in close association. both isolates produced leukotoxin and had identical electrophoretic patterns of dna fragments following cutting with restriction endonuclease haeiii. similarly pasteure ... | 2003 | 14733287 |
| immunologic responses of domestic and bighorn sheep to a multivalent pasteurella haemolytica vaccine. | the efficacy of a pasteurella haemolytica vaccine (serotypes a1, a2, and t10) to induce humoral antibodies and alter colonization of the upper respiratory tract by related p. haemolytica spp. strains was evaluated in 10 bighorn (ovis canadensis canadensis) and 10 domestic (ovis aries) sheep. sheep of each species were divided into five pairs based on age and history of respiratory disease. one sheep in each pair was vaccinated twice 2 wk apart with 2 ml of vaccine (vac group) and the remaining a ... | 1999 | 10231755 |
| attempted protection of bighorn sheep (ovis canadensis) from pneumonia using a nonlethal cytotoxic strain of pasteurella haemolytica, biotype a, serotype. | between february and april, 1994, we tested the hypothesis that bighorn sheep (ovis canadensis canadensis) inoculated with a cytotoxic isolate of pasteurella haemolytica biotype a, serotype 11 (a11) could withstand challenge inoculation with a cytotoxic strain of p. haemolytica a2 of domestic sheep origin known to cause lethal pneumonia in bighorn sheep. on experimental day o, two bighorn sheep were inoculated intratracheally with 6 x 10(9) colony forming units (cfu) of a cytotoxic strain of p. ... | 1996 | 8722270 |
| use of dna analysis of pasteurella haemolytica biotype t isolates to monitor transmission in bighorn sheep (ovis canadensis canadensis). | pneumonia has been identified as a major cause of poor lamb survival in indigenous herds of rocky mountain bighorn sheep (ovis canadensis canadensis) in central idaho. pasteurella haemolytica was isolated from five adult rocky mountain bighorn ewes captured from a free-ranging herd in central idaho. the lambs from two of these ewes delivered by cesarean section were free of p. haemolytica until 40 days of age and after repeated contact with their dams. the lambs subsequently developed signs of p ... | 1993 | 8385150 |
| fatal pneumonia following inoculation of healthy bighorn sheep with pasteurella haemolytica from healthy domestic sheep. | in a series of three experiments, isolates of pasteurella haemolytica biotype a, serotype 2, ribotype reference wsu-1, from healthy domestic sheep, were inoculated intratracheally into eight bighorn sheep (ovis canadensis canadensis) and seven domestic sheep with doses of bacteria ranging from 5.3 x 10(8) to 8.6 x 10(11) colony forming units. seven of eight inoculated bighorn sheep died from acute pneumonia within 48 hr of inoculation, whereas all seven domestic sheep inoculated with comparable ... | 1994 | 8028096 |
| detecting nonhemolytic pasteurella haemolytica infections in healthy rocky mountain bighorn sheep (ovis canadensis canadensis): influences of sample site and handling. | effects of sampling procedures on ability to culture pasteurella spp. from rocky mountain bighorn sheep (ovis canadensis canadensis) were examined experimentally. sample site influenced (p less than 0.0001) recovery of p. haemolytica in adult bighorn sheep. we isolated nonhemolytic p. haemolytica from 18 of 19 tonsillar swabs and 18 of 19 tonsillar biopsies from adult sheep, yet only four of 19 nasal swabs yielded isolates. sample handling also affected (p less than 0.0001) recovery of p. haemol ... | 1991 | 2023328 |
| using ribosomal rna gene restriction patterns in distinguishing isolates of pasteurella haemolytica from bighorn sheep (ovis canadensis). | pasteurella haemolytica isolates (n = 31) from two isolated captive herds of rocky mountain bighorn sheep (ovis canadensis canadensis) were characterized and compared phenotypically (biotype, serotype, hemolytic activity) and by a genomic fingerprinting method known as ribotyping. seven to nine distinct phenotypes were observed. depending on the method used for serotyping, one to three phenotypes were common to both herds. eighteen isolates, recovered from both herds, were non-hemolytic, biotype ... | 1992 | 1324994 |
| effects of delivery method on serological responses of bighorn sheep to a multivalent pasteurella haemolytica supernatant vaccine. | the safety and efficacy of a remotely delivered multivalent pasteurella haemolytica supernatant vaccine (serotypes a2 and t10) were examined in captive rocky, mountain bighorn sheep (ovis canadensis canadensis). twenty bighorn sheep were grouped according to baseline leukotoxin neutralizing antibody titers (< or =2 or >2 log2(-1)) and vaccination history (previously vaccinated or unvaccinated). within these groups, animals were randomly assigned to one of two delivery treatments: hand injection ... | 2000 | 10682747 |