| a basic residue at position 36p of the propeptide is not essential for the correct folding and subsequent autocatalytic activation of prochymosin. | position 36p in the propeptides of gastric aspartic proteinases is generally occupied by lysine or arginine. this has led to the conclusion that a basic residue at this position, which interacts with the active-site aspartates, is essential for folding and activation of the zymogen. lamb prochymosin has been shown by cdna cloning to possess glutamic acid at 36p. to investigate the effect of this natural mutation which appears to contradict the proposed role of this residue, calf and lamb prochym ... | 2001 | 11298755 |
| presence of shiga toxin-producing e. coli o157:h7 in a survey of wild artiodactyls. | this study was carried out to evaluate the role of wild artiodactyls as reservoirs of escherichia coli o157:h7 for livestock and humans. retroanal mucosal swabs samples from 206 red deer (cervus elaphus), 20 roe deer (capreolus capreolus), 6 fallow deer (dama dama) and 11 mouflon (ovis musimon), collected during the hunting season (autumn-winter) in south-western spain, were screened. samples were pre-enriched in modified buffered peptone water, concentrated by an immunomagnetic separation techn ... | 2007 | 17229534 |
| detection and characterisation of shiga toxin-producing escherichia coli other than escherichia coli o157:h7 in wild ruminants. | shiga toxin-producing escherichia coli (stec) are an important group of emerging pathogens, with ruminants recognised as their main natural reservoir. the aim of this work was to establish the prevalence of non-o157 stec in free-ranging wild ruminants in the extremadura region of spain and to characterise them phenogenotypically. faecal samples were collected from 243 wild ruminants, including cervus elaphus, capreolus capreolus, dama dama and ovis musimon and were examined for stec using both p ... | 2009 | 18337133 |
| characterization of esbl- and ampc-producing and fluoroquinolone-resistant enterobacteriaceae isolated from mouflons (ovis orientalis musimon) in austria and germany. | the aim of this study was to investigate the presence of β-lactamase producing or fluoroquinolone-resistant members of the family enterobacteriaceae in european mouflons (ovis orientalis musimon). the mouflon samples originated from nasal and perineal swabs and/or organ samples in cases of a suspected infection. only one of the 32 mouflons was tested positive for the presence of enterobacteriaceae that displayed either an esbl/ampc phenotype or were resistant to ciprofloxacin. the positively tes ... | 2016 | 27192164 |
| prevalence of shiga toxin-producing escherichia coli, salmonella spp. and campylobacter spp. in large game animals intended for consumption: relationship with management practices and livestock influence. | although wild ruminants have been identified as reservoirs of shiga-toxin producing escherichia coli (stec), little information is available concerning the role of salmonella spp. and campylobacter spp. in large game species. we evaluated the presence of these pathogens in faeces (n=574) and carcasses (n=585) sampled from red deer (n=295), wild boar (n=333) and other ungulates (fallow deer, mouflon) (n=9). animal sampling was done in situ from 33 hunting estates during two hunting seasons. salmo ... | 2013 | 23384892 |
| occurrence and characterization of stx and/or eae-positive escherichia coli isolated from wildlife, including a typical epec strain from a wild boar. | shiga toxin-producing e. coli (stec) and enteropathogenic e. coli (epec) strains are food-borne pathogens associated with acute diarrhea. haemolytic-uremic syndrome (hus) is often a complication of stec infection. in order to examine the occurrence, serotypes, virulence and antimicrobial-resistance profiles of stec and epec in wildlife, 326 faecal e. coli strains from 304 clinically healthy animals were analyzed. for this approach stx1, stx2 and eae genes, as well as accessory virulence determin ... | 2017 | 28757042 |