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paratuberculosis in cattle and free-living exotic deer.paratuberculosis was studied among dairy cows and exotic deer that shared grazing areas at point reyes national seashore, california. of the 10 dairy herds tested, 5 (50%) were infected with mycobacterium paratuberculosis (based on results of fecal culture). mycobacterium paratuberculosis was cultured from 9 (8.7%) of the 103 bovine fecal samples and from 4 (3.9%) of the 103 bovine rectal mucosa scapings tested. of 89 fecal samples from 52 axis deer (axis axis) and 37 fallow deer (dama dama), 5 ...1979500426
observations on diagnostic tests for paratuberculosis in a deer herd.paratuberculosis was diagnosed in a herd of 62 fallow deer (dama dama) and 38 sika deer (cervus nipon) maintained in an animal park in ohio. a comparison was made of delayed-type hypersensitivity responses, results of in vitro lymphocyte immunostimulation tests, and isolation of mycobacterium paratuberculosis on fecal culture in 19 deer.1979521371
evidence of paratuberculosis in ohio's white-tailed deer, as determined by an enzyme-linked immunosorbent assay.an enzyme-linked immunosorbent assay (elisa) was developed and used to detect antibodies to mycobacterium paratuberculosis in serum samples obtained in december of 1983 from 954 hunter-killed white-tailed deer (odocoileus virginianus) in 13 ohio counties. positive or negative status was determined by calculating a signal-to-noise ratio, a ratio between the optical density of the test serum and negative reference sera; a ratio of greater than or equal to 3.0 was considered positive. twenty-four s ...19863099612
paratuberculosis in free-ranging fallow deer in spain.paratuberculosis was diagnosed in a population of approximately 1,000 free-ranging fallow deer (dama dama) sampled from 1997-98 in the regional hunting reserve of el sueve (asturias, spain). five of eight animals observed with diarrhea were diagnosed as having paratuberculosis on the basis of gross lesions at postmortem examination and histopathology. in two deer, mycobacterium avium subsp. paratuberculosis was cultured and identified by polymerase chain reaction. indirect enzyme-linked immunoso ...200212238386
[mycobacterium avium subsp. paratuberculosis in wild animal species and cattle in styria/austria].infections with mycobacterium ovium ssp. paratuberculosis (m. paratuberculosis) are increasingly recognised worldwide. in addition to an increased prevalence of paratuberculosis in austrian cattle herds, recent years have also shown a rise in infections with m. paratuberculosis in wild red and roe deer, chamois and mouflon. during the period from june 2002 to september 2004, mesenteric lymph nodes were taken from a total of 483 wild animals hunted or found dead and from 338 deceased cattle. samp ...200516048043
quantification of mycobacterium avium subsp. paratuberculosis strains representing distinct genotypes and isolated from domestic and wildlife animal species by use of an automatic liquid culture system.quantification of 11 clinical strains of mycobacterium avium subsp. paratuberculosis isolated from domestic (cattle, sheep, and goat) and wildlife (fallow deer, deer, wild boar, and bison) animal species in an automatic liquid culture system (bactec mgit 960) was accomplished. the strains were previously isolated and typed using is1311 pcr followed by restriction endonuclease analysis (pcr-rea) into type c, s, or b. a strain-specific quantification curve was generated for each m. avium subsp. pa ...201222649014
mycobacterium avium subsp. paratuberculosis isolates induce in vitro granuloma formation and show successful survival phenotype, common anti-inflammatory and antiapoptotic responses within ovine macrophages regardless of genotype or host of origin.the analysis of the early macrophage responses, including bacterial growth within macrophages, represents a powerful tool to characterize the virulence of clinical isolates of mycobcaterium avium susbp. paratuberculosis (map). the present study represents the first assessment of the intracellular behaviour in ovine monocyte-derived macrophages (mdms) of map isolates representing distinct genotypes (c, s and b), and isolated from cattle, sheep, goat, fallow deer, deer, and wild boar. intracellula ...201425111300
sensitive and specific enzyme-linked immunosorbent assay for detecting serum antibodies against mycobacterium avium subsp. paratuberculosis in fallow deer.the enzyme-linked immunosorbent assay (elisa) is the diagnostic test most commonly used in efforts to control paratuberculosis in domestic ruminants. however, commercial elisas have not been validated for detecting antibodies against mycobacterium avium subsp. paratuberculosis in wild animals. in this study, we compared the sensitivities and specificities of five elisas using individual serum samples collected from 41 fallow deer with or without histopathological lesions consistent with paratube ...201424872517
mycobacterium avium subspecies paratuberculosis isolates from sheep and goats show reduced persistence in bovine macrophages than cattle, bison, deer and wild boar strains regardless of genotype.assessment of the virulence of isolates of mycobacterium avium subsp. paratuberculosis (map) exhibiting distinct genotypes and isolated from different hosts may help to clarify the degree to which clinical manifestations of the disease in cattle can be attributed to bacterial or to host factors. the objective of this study was to test the ability of 10 isolates of map representing distinct genotypes and isolated from domestic (cattle, sheep, and goat), and wildlife animal species (fallow deer, d ...201323415474
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