Publications

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species differentiation of lungworms (dictyocaulidae) by polymerase chain reaction/restriction-fragment-length polymorphism of second internal transcribed spacers of ribosomal dna.the bovine lungworm dictyocaulus viviparus is found in cattle and deer species (roe deer, fallow deer). although a separate lungworm species (d. eckerti) from roe deer has been described, this description, based on minor morphological differences, is not generally accepted. the aim of the present study was to examine genetic differences among four lungworm species isolated from cattle, sheep, horses, and fallow deer. ribosomal second internal transcribed spacers (its2) of individual worms of all ...19968738276
its2 sequences of dictyocaulus species from cattle, roe deer and moose in sweden: molecular evidence for a new species.total dna was isolated from adult lungworms of the genus dictyocaulus, collected from cattle, moose (alces alces) and roe deer (capreolus capreolus) in sweden. the second ribosomal internal transcribed spacer was amplified with pcr, and dna sequences were determined from nine individual worms that all came from different hosts in order to avoid analysis of siblings. the sequence data obtained were aligned and compared with similar data derived from german lungworm isolates from cattle and fallow ...199910428637
[deworming of corralled fallow deer (dama dama) using mebendazole].the efficacy of mebendazole on nematode infections in farmed fallow-deer (dama dama) was studied. the substance was administered as medicated feed, 6 mg/kg bodyweight during 10 days. at the same time the parasitic burden of the animals was investigated. a "springrise" of trichostrongylidae, trichuris and capillaria was present during late winter--early spring following by a "self-cure" reaction. a "springrise" phenomenon in early spring was also evident for chabertia and dictyocaulus viviparus b ...19846531211
[clinical examination of the blood of roe deer (capreolus capreolus l.) and fallow deer (dama dama l.) naturally invaded by parasites].roe-deer (capreolus capreolus l. -- five animals) and fallow deer (dama dama l. -- eleven animals) of both sexes and at different age were subject to blood examination. the deer were killed in several preserves in bohemia during autumn and winter shootings in two years. the following blood values were determined: erythrocyte count, leucocyte count, hemoglobin content, hematocrit values, mcv, mch, mchc, white blood picture, total protein, its fractions (albumin, alpha-, beta-, and gamma-globulin) ...1975809892
species-specific polymerase chain reaction for the differentiation of larvae from dictyocaulus viviparus and dictyocaulus eckerti.using substantial interspecific differences between the second internal transcribed spacer (its2) region within the rdna gene of dictyocaulus eckerti and dictyocaulus viviparus a species-specific pcr was developed to distinguish between lungworm larvae of the two species from fallow deer and cattle. it was found that the method of dna extraction was crucial for the sensitivity of the pcr. with serial dilutions of dna extracted from 10,000 larvae the its2 fragment could be amplified from all dilu ...19979066058
differences in a ribosomal dna sequence of lungworm species (nematoda:dictyocaulidae) from fallow deer, cattle, sheep and donkeys.the second internal transcribed spacer (its2)* of the ribosomal dna of dictyocaulus viviparus from cattle, d eckerti from fallow deer. d filaria from sheep and d arnfieldi from donkeys has been sequenced to investigate the genetic relationships among lungworm species, especially between d viviparus and d eckerti, because the latter is not generally accepted as a separate species. the length of the its2 varied between 403 and 481 bases, and its gc content ranged from 25 to 33 per cent. intraspeci ...19979160418
survey of internal parasitism and anthelmintic use in farmed deer.faecal samples and questionnaires from 115 and 130 farms respectively were used to survey the internal parasite status of the national deer herd and examine current drenching practices. the survey included farms with red deer and wapiti-red deer crosses (cervus elaphus), and fallow deer (dama dama). gastrointestinal nematode eggs were recorded from 84% of all farms, dictyocaulus viviparus larvae from 85% of all farms, and elaphostrongylus cervi larvae from 35% of the farms with c. elaphus. faeca ...198316030938
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