prevalence of infectious bovine rhinotracheitis in southern india. | the authors describe a serological survey on the prevalence of infectious bovine rhinotracheitis (ibr) among cattle and buffalo (bubalus bubalis) in three southern states of india. a local isolate of bovine herpesvirus 1 (bhv-1) from an outbreak of the respiratory form of ibr was used as a source of virus antigen in avidin-biotin enzyme-linked immunosorbent assay (elisa). the overall prevalence of antibodies to bhv-1 in cattle was 50.9%, and in buffalo was 52.5%. among breeding bulls, 114/120 sa ... | 1996 | 9025147 |
molecular characterisation of a field strain of bubaline herpesvirus isolated from buffaloes (bubalus bubalis) after pharmacological reactivation. | two healthy buffaloes (bubalus bubalis) in a herd which had not been vaccinated against infectious bovine rhinotracheitis (ibr), were selected for their seropositivity for anti-bovine herpesvirus type 1 (bohv-1) glycoprotein e antibodies, and injected intramuscularly daily with dexamethasone for five consecutive days (day 1 to day 5) to reactivate any latent herpesvirus. blood samples and nasal and vaginal swabs were collected daily from day 5 to day 15 from each buffalo for virological examinat ... | 2004 | 14979671 |
interferon-alpha genes from bos and bubalus bubalus. | interferon-a genes were cloned from six breeds of three species of two genera (three chinese native cattle breeds of yellow cattle, wild yak and huanhu domestic yak, one european breed of holstein cow, and two water buffalo breeds of fuan water buffalo and fuzhong water buffalo) by direct pcr. the pcr products were directly inserted into the expression vector to be sequenced and expressed. sequence analysis showed that ifn-a genes of six clones were composed of 498 nucleotides, encoding a mature ... | 2006 | 16621760 |
should the domestic buffalo (bubalus bubalis) be considered in the epidemiology of bovine herpesvirus 1 infection? | only limited information is available on the epidemiology and pathogenesis of bovine herpesvirus 1 (bohv-1) in domestic buffalos. in this study, a virulent bohv-1 field strain isolated from cattle was inoculated into buffaloes to evaluate their susceptibility to the virus and to investigate the establishment of viral latency through clinical, virological and serological investigations. latency was also studied by attempting viral reactivation using pharmacological induction. six of seven male, 5 ... | 2010 | 20206452 |
bovine herpesvirus type 1 marker vaccine induces cross-protection against bubaline herpesvirus type 1 in water buffalo. | water buffalo (bubalus bubalis) are susceptible to bovine herpesvirus type 1 (bohv-1) and a species-specific herpesvirus, bubaline herpesvirus type 1 (buhv-1). in this study, an attenuated marker bohv-1 based vaccine against buhv-1 challenge was evaluated to determine whether it induces protection from viral replication. one group of water buffalo calves was immunized with an attenuated bohv-1 marker vaccine. a second group was not vaccinated and used as the control. during the post-vaccination ... | 2014 | 24985155 |
first report of isolation and molecular characterization of bubaline herpesvirus 1 (buhv1) from argentinean water buffaloes. | herpesviruses have mainly co-evolved with their hosts for millions of years. however, bovine herpesvirus 1 (bohv1) and related ruminant alphaherpesviruses have been reported to cross the species barrier. bubaline herpesvirus 1 (buhv1) is an alphaherpesvirus closely related to bohv1 and bohv5. according to the serological cross-relationships between ruminant alphaherpesviruses, several surveys have studied the occurrence of bohv1-related virus infection in wild and domestic ruminant species. rece ... | 2014 | 24938487 |
seroepidemiology of infection withneospora caninum, leptospira, and bovine herpesvirus type 1 in water buffaloes(bubalus bubalis)in veracruz, mexico. | we aimed to determine the seroprevalence of infection withneospora caninum, leptospira, and bovine herpesvirus type 1 and risk factors associated with these infections in water buffaloes in veracruz state, mexico. through a cross-sectional study, 144 water buffaloes(bubalus bubalis)raised in 5 ranches of veracruz were examined for anti-n.caninumand anti-bovine herpesvirus type 1 antibodies by enzyme immunoassays, andanti-leptospira interrogansantibodies by microscopic agglutination test. of the ... | 2017 | 29403656 |
isolation and identification of bubaline herpesvirus 1 (buhv-1) from latently infected water buffalo (bubalus bubalis) from iran. | in order to isolate buffaloes herpesvirus 1 (buhv-1) from latently infected water buffalo (bubalus bubalis), 16 buffalo heifers were selected from a herd. at first, animals were bled and their sera were tested by virus neutralization (vn) test, using bovine herpesvirus 1 (bohv-1). according to the results of vn test and dexamethasone injection (0.1 mg/kg bw) for 5 consecutive days, the examined buffaloes were divided into 4 groups. vaginal and nasal swabs were daily collected from all buffaloes ... | 2020 | 31313017 |
reactive oxygen metabolites in alpha-herpesvirus-seropositive mediterranean buffaloes (bubalus bubalis): a preliminary study. | in the present study on bubalus bubalis of the campania region (italy) the serum levels of derivatives of reactive oxygen metabolites (d-roms), anti-rom and oxidative stress index (osi) were evaluated. these data were then related to the seropositive status of the animals against alpha-herpesviruses, precisely bubaline herpesvirus 1 (buhv-1) and bovine herpesvirus 1 (bohv-1). clinically healthy mediterranean buffaloes were selected for this study. the serum samples of these animals were taken, a ... | 2018 | 30468352 |
evaluation of safety and efficacy of an inactivated marker vaccine against bovine alphaherpesvirus 1 (bohv-1) in water buffalo (bubalus bubalis). | recent studies have explored the seropositivity of bovine alphaherpesvirus 1 (bohv-1) in water buffaloes, suggesting the urgency for developing strategies to eradicate the virus involving both cattle and water buffaloes. however, in europe, the glycoprotein e (ge) deleted marker vaccines against bohv-1 are commercially available only for the cattle industry. this study, for the first time, evaluated the safety and efficacy of a commercial inactivated ge-deleted marker vaccine in water buffalo. f ... | 2021 | 33917160 |