| development and evaluation of real-time pcr assay for the detection of babesia orientalis in water buffalo (bubalus bubalis, linnaeus, 1758). | abstract babesia orientalis is the causative agent of babesiosis in water buffalo (bubalus babalis, linnaeus, 1758). in this study, a taqman real-time pcr assay was developed for quantitative detection of b. orientalis in water buffalo. hybridization probe and oligonucleotide primers were designed based on the v4 region of 18s rrna gene. detection limit was determined at 2 parasites. blood samples were collected from experimentally infected water buffalo, as well as from 180 field samples, which ... | 2011 | 21711103 |
| characterization and annotation of babesia orientalis apicoplast genome. | babesia orientalis is an obligate intraerythrocytic protozoan parasite of the buffalo (bubalus bubalis, linnaeus, 1758) transmitted by the tick rhipicephalus heamaphysaloides. it is the causative agent of water buffalo babesiosis, one of the most important pathogens of water buffalo in central and southern china. as a member of the phylum apicomplexa, b. orientalis possesses a relatively independent and alga originated organelle the apicoplast. apicoplasts in other apicomplexa parasites are invo ... | 2015 | 26474853 |