| Diversity of Beauveria spp. isolates from pollen beetles Meligethes aeneus in Switzerland. | Pollen beetles Meligethes aeneus were collected in oilseed rape fields at different sites in Switzerland in spring 2004-2005 and 32 isolates of the fungal genus Beauveria occurring as latent infections in the beetles were obtained and molecularly characterized. Three major clades, Beauveria bassiana sensu stricto (Clade A: n=13), Beauveriabrongniartii (Clade B: n=1) and Beauveria Clade C (n=18) were identified among the isolates based on sequences of the ITS region and the 5' end of EF1-a. B. ba ... | 2012 | 22008375 |
| rna-sequencing analysis reveals abundant developmental stage-specific and immunity-related genes in the pollen beetle meligethes aeneus. | the pollen beetle (meligethes aeneus) is a major pest of oilseed rape (brassica napus) and other cruciferous crops in europe. pesticide-resistant pollen beetle populations are emerging, increasing the economic impact of this species. we isolated total rna from the larval and adult stages, the latter either naïve or immunized by injection with bacteria and yeast. high-throughput rna sequencing (rna-seq) was carried out to establish a comprehensive transcriptome catalogue and to screen for develop ... | 2014 | 24252113 |
| facilitating co-existence by tracking gene dispersal in conventional potato systems with microsatellite markers. | based on international findings, irish co-existence guidelines for the cultivation of gm potato stipulate that an isolation distance of 20 m is required to minimize the spread of transgenic pollen in accordance with required labeling thresholds. as potato tolerant to phytophthora infestans is the most applicable gm crop from an irish context, we tested the efficacy of this isolation distance under irish environmental conditions using the conventional variety désirée as a pollen donor and the mal ... | 2008 | 18289498 |
| predatory beetles feed more pest beetles at rising temperature. | climate warming is a challenge for many plants and animals as they have to respond to rising temperature. rising temperature was observed to affect herbivores and predators. activity-density of abundant predatory carabid beetles, which are considered important natural control agents of agricultural pests, was observed to increase at rising temperature. the pollen beetle meligethes aeneus is one of the most important insect pests in european oilseed rape fields, and its larvae were observed to be ... | 2016 | 27084775 |
| the effect of a piperonyl butoxide/tau-fluvalinate mixture on pollen beetle (meligethes aeneus) and honey bees (apis mellifera). | previous work has characterised pyrethroid resistance in pollen beetle (meligethes aeneus f.) as principally an oxidative mechanism. piperonyl butoxide (pbo) can synergise this resistance in the field, but its effects on the honey bee are thought to be unacceptable. | 2012 | 22228561 |
| migratory flight behaviour of the pollen beetle meligethes aeneus. | the field ecology of the pollen beetle meligethes aeneus and its damaging effects on oilseed rape crops are well understood. however, the flight behaviour of m. aeneus, in particular the drivers for migratory movements across the landscape, is not well studied. we combined three established methodologies - suction traps, vertical-looking radar and high-altitude aerial netting - to demonstrate that m. aeneus flies at a range of altitudes at different points during its active season. | 2017 | 28195419 |
| the potential of decision support systems to improve risk assessment for pollen beetle management in winter oilseed rape. | the reliance on and extensive use of pyrethroid insecticides have led to pyrethroid resistance in pollen beetle (meligethes aeneus). widespread adoption of best practice in pollen beetle management is therefore needed. decision support systems (dsss) that identify the risk period(s) for pest migration can help to target monitoring and control efforts, but they must be accurate and labour efficient to gain the support of growers. weather data and the phenology of pollen beetles in 44 winter oilse ... | 2016 | 26112069 |
| insecticide resistance in pollen beetles over 7 years - a landscape approach. | in spite of considerable interest in the impact of pesticides on pest populations, few attempts have been made to link resistance patterns of insect pests to land-use features across spatial and temporal scales. we hypothesise that pollen beetle pesticide resistance increases in areas with a high proportion of oilseed rape and with an even mixture of winter and spring oilseed rape owing to high pesticide selection pressure in such areas. | 2016 | 26033304 |
| insight into the meligethes aeneus voltage-sensitive sodium channel structure and an attempt to select the best pyrethroid ligands. | the voltage-sensitive sodium channel (vssc) is a target for the pharmacological action of pyrethroids which are used in controlling pests, including those of agricultural importance. among these is the pollen beetle (meligethes aeneus f.) - the most serious pest of brassica napus. owing to the heavy use of pyrethroids, a widespread build-up of resistance has occurred. the main cause of pyrethroid insensitivity in m. aeneus is considered to be an increased oxidative metabolism; however, the addit ... | 2016 | 25652001 |
| an individual-based model of the evolution of pesticide resistance in heterogeneous environments: control of meligethes aeneus population in oilseed rape crops. | preventing a pest population from damaging an agricultural crop and, at the same time, preventing the development of pesticide resistance is a major challenge in crop protection. understanding how farming practices and environmental factors interact with pest characteristics to influence the spread of resistance is a difficult and complex task. it is extremely challenging to investigate such interactions experimentally at realistic spatial and temporal scales. mathematical modelling and computer ... | 2014 | 25531104 |
| manipulating feeding stimulation to protect crops against insect pests? | enhancing natural mechanisms of plant defense against herbivores is one of the possible strategies to protect cultivated species against insect pests. host plant feeding stimulation, which results from phagostimulant and phagodeterrent effects of both primary and secondary metabolites, could play a key role in levels of damage caused to crop plants. we tested this hypothesis by comparing the feeding intensity of the pollen beetle meligethes aeneus on six oilseed rape (brassica napus) genotypes i ... | 2014 | 25355636 |
| temperature-activity relationships in meligethes aeneus: implications for pest management. | pollen beetle (meligethes aeneus f.) management in oilseed rape (brassica napus l.) has become an urgent issue in the light of insecticide resistance. risk prediction advice has relied upon flight temperature thresholds, while risk assessment uses simple economic thresholds. however, there is variation in the reported temperature of migration, and economic thresholds vary widely across europe, probably owing to climatic factors interacting with beetle activity and plant compensation for damage. ... | 2015 | 25052810 |
| a survey of pyrethroid-resistant populations of meligethes aeneus f. in poland indicates the incidence of numerous substitutions in the pyrethroid target site of voltage-sensitive sodium channels in individual beetles. | the pollen beetle (meligethes aeneus f.) is the most devastating pest of oilseed rape (brassica napus) and is controlled by pyrethroid insecticides. however, resistance to pyrethroids in europe is becoming widespread and predominant. pyrethroids target the voltage-sensitive sodium channel (vssc), and mutations in vssc may be responsible for pyrethroid insensitivity. here, we analysed individual beetles that were resistant to esfenvalerate, a pyrethroid, from 14 populations that were collected fr ... | 2014 | 24974912 |
| a de novo transcriptome of european pollen beetle populations and its analysis, with special reference to insecticide action and resistance. | the pollen beetle meligethes aeneus is the most important coleopteran pest in european oilseed rape cultivation, annually infesting millions of hectares and responsible for substantial yield losses if not kept under economic damage thresholds. this species is primarily controlled with insecticides but has recently developed high levels of resistance to the pyrethroid class. the aim of the present study was to provide a transcriptomic resource to investigate mechanisms of resistance. cdna was seq ... | 2014 | 24707894 |
| target-site resistance to pyrethroid insecticides in german populations of the cabbage stem flea beetle, psylliodes chrysocephala l. (coleoptera: chrysomelidae). | cabbage stem flea beetle, psylliodes chrysocephala l. (coleoptera: chrysomelidae) is a major pest of winter oilseed rape in several european countries particularly attacking young emerging plants in autumn. over the last several decades, pyrethroid insecticides have been foliarly applied to control flea beetle outbreaks. recent control failures in northern germany suggested pyrethroid resistance development in cabbage stem flea beetles, which were confirmed by resistance monitoring bioassays usi ... | 2014 | 24485308 |
| molecular and functional characterization of cyp6bq23, a cytochrome p450 conferring resistance to pyrethroids in european populations of pollen beetle, meligethes aeneus. | the pollen beetle (meligethes aeneus f.) is widespread throughout much of europe where it is a major coleopteran pest of oilseed rape (brassica napus). the reliance on synthetic insecticides for control, particularly the pyrethroid class, has led to the development of populations with high levels of resistance. resistance to pyrethroids is now widespread throughout europe and is thought to be mediated by enhanced detoxification by cytochrome p450ś and/or mutation of the pyrethroid target-site, t ... | 2014 | 24316412 |
| does insecticide application in a winter oilseed rape field influence the abundance of pollen beetle meligethes aeneus in nearby ornamental flowers and vegetables? | pollen beetle is a pest that attacks oilseed rape as well as many other brassicaceous crops, garden vegetables and ornamental flowers. the present study was primarily carried out to investigate whether insecticide application in brassicaceous field crops might influence the abundance of pollen beetles in nearby private garden flowers and vegetables. | 2013 | 23519894 |
| incidence of pyrethroid-resistant oilseed rape pests in germany. | failures in pollen beetle control using pyrethroids since 2005 indicated pyrethroid resistance in germany. therefore, resistance monitoring using bioassays was established in germany for oilseed rape pest insects. | 2013 | 23345124 |
| pyrethroid resistance and thiacloprid baseline susceptibility of european populations of meligethes aeneus (coleoptera: nitidulidae) collected in winter oilseed rape. | pollen beetle, meligethes aeneus f. (coleoptera: nitidulidae), is a major pest in european winter oilseed rape. recently, control failures with pyrethroid insecticides commonly used to control this pest have been reported in many european countries. for resistance management purposes, the neonicotinoid insecticide thiacloprid was widely introduced as a new mode of action for pollen beetle control. | 2011 | 21394884 |
| crop-noncrop spillover: arable fields affect trophic interactions on wild plants in surrounding habitats. | ecosystem processes in agricultural landscapes are often triggered by resource availability in crop and noncrop habitats. we investigated how oilseed rape (osr; brassica napus, brassicaceae) affects noncrop plants in managed systems and semi-natural habitat, using trophic interactions among wild mustard (sinapis arvensis, brassicaceae), rape pollen beetles (meligethes aeneus, nitidulidae) and their parasitoids (tersilochus heterocerus, ichneumonidae). we exposed wild mustard as phytometer plants ... | 2011 | 21153737 |
| characterising metabolic resistance in pyrethroid-insensitive pollen beetle (meligethes aeneus f.) from poland and switzerland. | pollen beetle (meligethes aeneus f.) has become the most important pest of oilseed rape in europe, but its control has been greatly hindered by pyrethroid resistance. target-site resistance has been implicated previously, and, whilst synergism has been found with piperonyl butoxide (pbo), the exact nature of metabolic resistance has remained unknown. the use of pbo, in conjunction with its analogue en 16/5-1, has allowed the characterisation of metabolic resistance. | 2011 | 21104794 |
| species-specific primers for predation studies of the pollen beetle, meligethes aeneus (coleoptera, nitidulidae). | species-specific primers were developed for the pollen beetle (a pest in oilseed rape) for studies of predation by natural insect predators. two forward and three reverse primers were designed within the mitochondrial coi gene and used in combination to amplify fragments in the size range of 163-290 bp. remains of pollen beetle dna were consistently detected in pardosa spiders up to 24 h after ingestion but dropped drastically at 48 h. these primers will facilitate studies on biological control ... | 2009 | 21564852 |
| orientation and feeding responses of the pollen beetle, meligethes aeneus, to candytuft, iberis amara. | the pollen beetle, meligethes aeneus, which is an important pest of oilseed rape, brassica napus, and turnip rape, b. rapa var. campestris, does not oviposit in all species of the brassicaceae. the relationship between m. aeneus and candytuft, iberis amara (brassicacae), was investigated as part of chemical ecological studies into the development of control methods employing non-host-derived repellents. in choice and nonchoice feeding tests, m. aeneus completely rejected i. amara. however, in a ... | 2004 | 15274439 |
| establishment of the amplified fragment length polymorphism (aflp) technique for genotyping of pollen beetle (meligethes aeneus)--a noxious insect pest on oilseed rape (brassica napus). | in order to conduct studies concerning genetic variability of pollen beetles (meligethes aeneus), a genotyping protocol was established. no genome information is available for pollen beetles so the amplified fragment length polymorphism (aflp) technique was chosen since it does not depend on any prior sequence information of the samples and also is a sensitive and robust technique. however, several modifications were needed in order to adapt the method for analysis of pollen beetles. basic modif ... | 2004 | 15040453 |
| insecticide-resistant pollen beetles (meligethes aeneus f) found in danish oilseed rape (brassica napus l) fields. | the pollen beetle is the most important pest in danish oilseed rape fields. in 2001, we screened a broad range of pollen beetle populations for pyrethroid and dimethoate resistance. a standard dip-test was used to test insecticide resistance in 18 populations collected from oilseed winter and spring rape fields. the beetles were treated with four different insecticides: the pyrethroids tau-fluvalinate, lambda-cyhalothrin and esfenvalerate, and the organophosphate dimethoate. the results show tha ... | 2003 | 12974359 |
| landscape structure and biological control in agroecosystems | biological pest control has primarily relied on local improvements in populations of natural enemies, but landscape structure may also be important. this is shown here with experiments at different spatial scales using the rape pollen beetle (meligethes aeneus), an important pest on oilseed rape (brassica napus). the presence of old field margin strips along rape fields was associated with increased mortality of pollen beetles resulting from parasitism and adjacent, large, old fallow habitats ha ... | 1999 | 10436158 |
| differentiation and adaptation in brassica nigra populations: interactions with related herbivores. | local adaptation and population differentiation of plants are well documented, but studies on interactions with natural enemies are rare. in particular, evidence for plant adaptation to the local biotic environment, such as herbivores remains poor. we used the black mustard brassica nigra, an annual species of river valley and coastal habitats to (1) analyse population differentiation in plant traits and herbivory in a common garden experiment, (2) examine home versus away differences in a recip ... | 2011 | 20960010 |