| some properties of the lipase of geotrichum candidum evaluated by a fluorimetric assay technique. | | 1976 | 7541 |
| beta-glucosidases from fungus geotrichum candidum. | beta-glucosidases from geotrichum candidum 3c cellulase preparation were separated from c1 enzymes and beta-1,4-glucanases by means of deae-sephadex a-50 chromatography, gel filtration through p-150 biogel and chromatography on cm-cellulose, and then were fractionated by isoelectric focusing using carrier ampholites with ph ranges 3-6 and 4-6. beta-glucosidases with pi 3.8, 4.2, 4.6, 5.1, 5.6 and 6.2 were found in cellulase preparation from g. candidum 3c. molecular weight of beta-glucosidases w ... | 1977 | 15651 |
| oospora lactis strain actively producing lipase. | morphological, cultural and physiological properties of the fungus oospora lactis producing lipase are described. the activity of lipase depends on the composition of a growth medium, ph, temperature, the duration of cultivation. optimum conditions for lipase biosynthesis by the fungus have been found. the strain produces extracellular and endocellular lipases during submerged cultivation in the optimum growth medium at a high rate. | 1977 | 20556 |
| hyphal wall growth in neurospora crassa and geotrichum candidum. | growth of the walls of hyphae of neurospora crassa and geotrichum candidum was studied using longitudinal and serial transverse sectioning methods. rigidification of the hyphal wall below the extension zone did not appear to involve the gross formation of a secondary wall since the transition from extensible to non-extensible wall was not associated with an increase in thickness. however, behind the extension zone the walls leading hyphae of n. crassa increased in thickness until eventually they ... | 1975 | 128612 |
| kinetics and morphology of glucose-limited cultures of moulds grown in a chemostat and on solid media. | the affinity (ks value) of geotrichum candidum for glucose determined from chemostate cultures was ca. 1 mg/1. ks values for glucose was also estimated from the radial growth rates of colonies of g. candidum and neurospora crassa grown on media solidified with agar or silica gel. an assessment is made of the use of colony radial growth rate to determine substrate affinities. the length of apical and intercalary hyphal comparte ments, internode length and the diameter of leading hyphaat the margi ... | 1975 | 168829 |
| a model for hyphal growth and branching. | a mathematical model for hyphal growth and branching is described which relates cytological events within hyphae to mycelial growth kinetics. essentially the model quantifies qualitative theories of hyphal growth in which it is proposed that vesicles containing wall precursors and/or enzymes required for wall synthesis are generated at a constant rate throughout a mycelium and travel to the tips of hyphae where they fuse with the plasma membrane, liberating their contents into the wall and incre ... | 1979 | 379276 |
| [effect of fungal cellulases on enzymic activity of malt]. | in order to increase malt enzymic activity, cellulases from the fungus geotrichum candidum were used. malt milk was processed for 1--3 hr at 40 degrees c and ph 6.0--6.5 by the fungal preparation or culture fluid filtrate used in such quantities in which there were 0.5 to 9.0 units of cellulase activity per g malt. during the process enzymic activities of malt increased significantly: dextrinating activity--by 73%, amylolytic activity--by 60--100%, saccharifying activity--by 3.4 times, and prote ... | 1979 | 395535 |
| [thermal inactivation and storage behavior of technologically important enzymes. ii. lipase from geotrichum candidum and lipoxygenase from soybeans]. | the thermal inactivation of lipase out of geotrichum candidum and of lipoxigenase out of soybeans was investigated in phosphate buffer solution. comparable to peroxidase, a sharp bend was observed in the inactivation curves of both enzymes. the z-value of 19 degrees c for lipase out of geotrichum candidum was higher than the values indicated in the pertinent literature for pancreas lipase and milk lipase. the z-value for soy lipoxigenase was found to be 9.8 degrees c. | 1977 | 408985 |
| synthesis of various kinds of esters by four microbial lipases. | ester synthesis by microbial lipases, using homogeneous enzyme preparations, were investigated. the amount of synthesized ester was estimated by alkalimetry, and products were identified by thin-layer chromatography and infrared spectroscopy. lipases from aspergillus niger, rhizopus delemar, geotrichum candidum and penicillium cyclopium synthesized esters from oleic acid and various primary alcohols. only geotrichum candidum lipase synthesized esters of secondary alcohols. esters of tertiary alc ... | 1979 | 508776 |
| pancreatic and microbial lipases: a comparison of the interaction of pancreatic colipase with lipases of various origins. | conjugated bile salts inhibit the the hydrolysis of triglycerides (tg) by the lipases from rhizopus arrhizus and geotrichum candidum. this occurs for detergent concentrations similar to those which suppress the action of mammalian pancreatic lipases upon the same substrates. however, in opposition with what is observed with the latter enzymes, the activity is not restored by the addition of pancreatic colipase. both pancreatic and r. arrhizus lipases are inactivated at tributyrin/water interface ... | 1977 | 558492 |
| [black hairy tongue and geotrichum candidum (author's transl)]. | | 1977 | 560019 |
| [comparison of cell body and cell wall compositions between the yeast-like and mycelial phases of geotrichum candidum (author's transl)]. | | 1978 | 567704 |
| [utilization of geotrichum candidum to increase feeding value of corn cobs]. | | 1979 | 572468 |
| deamination of glumatic and aspartic acids by geotrichum candidum. | strains of geotrichum candidum were isolated from the surface of a commercial sample of limburger cheese and from raw milk. growth of the isolates in an acidified tryptone-yeast extract medium was accompanied by a rise in the ph of the medium from 3.5 to above 7.0 ammonia production, as indicated by nesslerization, was associated with the deamination of glutamic and aspartic acids. the first reaction in the production of ammonia from glutamic acid, isomerization to beta-methylaspartic acid, requ ... | 1979 | 582176 |
| fungal antigens as a source of sensitization and respiratory disease in scottish maltworkers. | mycological and serological studies were carried out as part of a survey of respiratory disease in scottish maltworkers. 70% of stained sputum smears from 574 workers showed the presence of higher plant cells and/or myclelia, and the spores of common environmental fungi. penicillium spp. (90%), rhizopus stolonifer (48%) and yeasts (53%) were the dominant fungi in 699 sputum cultures, and showed a similar proportional distribution in 327 samples of grain, malt, culms and dusts from fifty-six malt ... | 1977 | 589785 |
| carbohydrate composition and taxonomy of the genus dipodascus. | carbohydrates released during acid hydrolysis of intact cells of dipodascus were studied by gas-liquid chromatographic analysis as their trimethylsilyl derivatives. in addition, cells were characterized by pyrolysis gas-liquid chromatography and pyrolysis mass spectrometry. the data obtained support the classification of dipodascus uninucleatus in a separate genus dipodascopsis. glucuronic acid is present in d. uninucleatus and, therefore, a possible affinity to fungi classified in the zygomycet ... | 1977 | 603234 |
| [glyceride structure of fats. 11. structure studies on selected nut fats using lipases]. | using pancreatic lipase of geotrichum candidum, the authors determined the fatty acid pattern and the glyceride structure of peanut, filbert, walnut, brazil nut and cashew-nut fats. both the fatty acid pattern and the glyceride structure were comparable to those of other vegetable fats of corresponding total fatty acid composition. | 1978 | 692647 |
| geotrichum septicemia. | although disseminated geotrichosis is an unusual disease (to our knowledge, only six cases have been reported), geotrichum candidum is not a very virulent fungus. in our case, there was neutrophillic phagocytosis of the arthrosphores of g candidum, with rapid clearance of the fungus from the plasma of a chronically ill patient whose immune defenses were still intact. | 1976 | 814251 |
| conidiogenesis in pathogenic hyphomycetes. i. sporothrix, exophiala, geotrichum and microsporum. | conidium formation in 5 species of pathogenic hyphomycetous fungi, sporothrix schenckii, exophiala salmonis, e. sp., geotrichum candidum and microsporum canis were examined using light and scanning electron microscopy. the fungi, grown in pure culture on potato dextrose agar under defined conditions, demonstrated 4 kinds of conidiogenesis. the taxonomic value of the developmental characters in the classification of the fungi imperfecti is discussed. | 1976 | 944466 |
| crystallographic data and circular dichroism spectrum of lipase from geotrichum candidum link. | | 1975 | 1240147 |
| mycotic flora in the lower digestive tract of feral pigeons (columba livia) in the el paso, texas area. | fourteen species of fungus were isolated from the lower digestive tract of 39 of 80 pigeons. sixteen pigeons had concurrent isolations while two harbored three species. fungi isolated were allescheria boydii, aspergillus spp., candida krusei, chrysosporium spp., geotrichum candidum, mucor spp., paeciliomyces spp., penicillium spp., rhizopus spp., rhodotorula spp., scopulariopsis spp., streptomyces spp., and trichosporon cutaneum. there was no apparent evidence that these fungi were associated wi ... | 1976 | 1255918 |
| cutaneous geotrichosis in the red flamingo (phoenicopterus ruber). | in three flamingos (phoenicopterus ruber), which died showing extensive necrotic inflammation of the skin of the webs and the legs, the presence of abundant mycelium and arthrospores was shown in the altered dermis and epidermis. from all the lesions geotrichum candidum was isolated in pure culture. inoculation of suspensions of the fungus into domestic chickens and mice produced granulomas or more purulent nodules with central growth of abundant mycelium. from 20 of 28 experimental animals, g. ... | 1976 | 1265572 |
| chemical control of sour rot of oranges caused by geotrichum candidum. | | 1975 | 1241908 |
| changes in ascorbic acid and carbohydrate contents in tomato fruits infected with pathogens. | tomato fruits var romer vfn, inoculated with fusarium equiseti, f. chlamydosporium, geotrichum candidum, acremonium recifei, aspergilus flavus and a. niger showed decline in ascorbic acid content with days of incubation when compared with control (uninoculated) fruits. the total soluble sugars of the inoculated fruits also showed a reduction trend as the storage period was prolonged, but slight increase was observed on the 6th day after inoculation. | 1992 | 1438080 |
| isolation of chrysophanic acid-9-anthrone, the major antifungal principle of cassia tora. | an antifungal principle of defatted seed power of cassia tora linn. was isolated by extraction of an aqueous paste of the powder with benzene, followed by column chromatography over activated silica gel c using chloroform as the developing solvent. besides chrysophanic acid and other hydroxyanthraquinone derivatives, the major antifungal compound was identified as chrysophanic acid-9-anthrone, the structure of which was assigned on the basis of its chemical properties and uv, ir, nmr and mass sp ... | 1975 | 1143018 |
| yeast-like fungi recovered from normal human skin in nsukka (nigeria). | the incidence of yeast-like fungi on normal skin was investigated in 52 healthy nsukka individuals comprising 21 adults, 24 children, and 7 infants. eighty-six isolates of yeasts and 2 of geotrichum candidum were recovered. the yeasts included candida tropicalis-36 isolates, c. guilliermondii-3, c. parapsilosis-2, candida spp. (unidentified)-16, cryptococcus albidus-6, trichosporon cutaneum-7, trichosporon spp.-8 and rhodotorula spp.-8 isolates. the isolates originated from various sites, predom ... | 1976 | 1085120 |
| separation and characterization of 61- and 57-kda lipases from geotrichum candidum atcc 66592. | two lipolytic proteins (61 and 57 kda) present in a sephadex g-100 fraction of extracellular lipase from geotrichum candidum atcc 66592 were separated using high-performance liquid chromatography. crossed electrofocusing immunoelectrophoresis was used to demonstrate that the 61-kda lipase fraction contained two forms of lipase with pi 4.5 and 4.7. however, when deglycosylated with endoglycosidase h, the two forms gained an identical pi, 4.6. the 57-kda lipase fraction contained one form of lipas ... | 1992 | 1581866 |
| cloning and nucleotide sequences of two lipase genes from candida cylindracea. | two lipase-encoding genes (lip1 and lip2) have been isolated from a saci genomic library of the yeast candida cylindracea and their nucleotide sequences have been determined. comparison with the sequence of a cdna ruled out the presence of introns in the two genes. both orfs encode for mature proteins of 534 residues with putative signal peptides of 15 and 14 amino acids, respectively. when compared with other lipase sequences, the two c. cylindracea lipases showed homology only with the geotric ... | 1992 | 1610906 |
| [method of obtaining enzymatic preparations of lipase from the fungi of the genus geotrichum under semi-industrial conditions]. | a method of manufacturing stable enzymic preparations of lipase from fungi geotrichum candidum and g. asteroides under semi-industrial conditions has been developed. the paper describes the preparation of the inoculate, fermentation nutrient medium, scheme of cultivation and isolation of the raw enzyme with a yield of 31.2%. | 1976 | 1036115 |
| nuclei, septation, branching and growth of geotrichum candidum. | a study was made of growth, septation and branching in geotrichum candidum, a mould which forms physiologically complete septa. a correlation was observed between septation and branch initiation; branches were almost invariably formed just behind septa. primary branches and their parent intercalary compartments initially increased in length at an exponential rate before eventually attaining a constant rate of extension. the whole branching system (which eventually contained seven tips) produced ... | 1976 | 1034669 |
| isolation of fungi belonging to the genera geotrichum and trichosporum from human dermal lesions. | isolates of geotrichum and trichosporum spp. obtained from patients with a variety of dermal lesions were studied. among 2,202 cases examined, microorganisms of these genera were recovered from 100 (4,5%); there were 38 isolated of geotrichum- and 62 of trichosporum- spp. most isolations were obtained from nails: 52 cases. the species most frequently found were t. beigelii (25 cases) and g. candidum (30 cases). in 50 of the patients, these fungi were isolated in pure culture, in an additional 40 ... | 1976 | 987537 |
| [role of phagocytic cells in destruction of the yeast-like fungus geotrichum candidum]. | | 1976 | 987529 |
| lipolytic fermentations of stickwater by geotrichum candidum and candida lipolytica. | stickwater, a by-product of the fish meal and oil industry, is an aqueous suspension of fish proteins, lipids, and other materials, and also contains soluble nonprotein nitrogen but not carbohydrate. it is usually partially evaporated by heat to a marketable form called "fish solubles," which is sold with an acid preservative as an animal feed supplement. however, fish solubles are only used to a limited extent in feeds, because the lipids of solubles (averages 11%) are relatively prone to oxida ... | 1976 | 944554 |
| multiple crystal forms of lipases from geotrichum candidum. | multiple stable crystal forms of two lipases from the fungus geotrichum candidum have been obtained. the diffraction pattern extends to beyond 2.0 a resolution. similarity of the cell dimensions of various forms suggested similar packing of molecules in different crystals. this was confirmed by rotation function results. four heavy-atoms derivatives have been identified. | 1991 | 1870119 |
| allergic alveolitis due to wood-rot fungi. | farmer's lung is rare in australia whereas allergic alveolitis due to domestic fungal exposure ("domestic allergic alveolitis") is common. however there has been no systematic analysis of the fungi responsible for domestic allergic alveolitis in australia. the aim of this study was to determine the sources and types of fungi that cause this type of alveolitis in australia. twelve subjects with recently diagnosed, biopsy proved, alveolitis thought on clinical grounds to be due to domestic fungal ... | 1991 | 2060786 |
| ser-his-glu triad forms the catalytic site of the lipase from geotrichum candidum. | the ser-his-asp triad is a well known structural feature of the serine proteases. it has also been directly observed in the catalytic sites of two lipases, whose high-resolution three-dimensional structures have been determined 1,2. lipases show a wide variety of sizes, substrate and positional specificities, and catalytic rates 3. they achieve maximal catalytic rates at oil-water interfaces. the fungus geotrichum candidum produces several different forms of lipases, two of which have been purif ... | 1991 | 2062369 |
| enzyme-linked immunosorbent assay for detection of molds in cheese and yogurt. | an enzyme-linked immunosorbent assay was developed for the detection of molds in dairy products. new zealand white female rabbits were immunized with .45 mg of partially purified extracellular antigen from freeze-dried culture filtrates of aspergillus versicolor, cladosporium herbarum, geotrichum candidum, mucor circinelloides, and penicillium chrysogenum. blood was drawn at various intervals, and antibodies were separated and purified. antibody-peroxidase conjugates were prepared with the follo ... | 1990 | 2099362 |
| effect of cetylpyridinium chloride on pathogenic fungi and nocardia asteroides in sputum. | the effect of cetylpyridinium chloride (cpc) on pathogenic fungi and nocardia asteroides was studied. sputa seeded with each of 11 organisms (aspergillus flavus; aspergillus fumigatus, blastomyces dermatitidis, candida albicans, coccidioides immitis, cryptococcus neoformans, geotrichum candidum, histoplasma capsulatum; nocardia asteroides, paracoccidioides brasiliensis, and sporothrix schenckii) were treated with cpc and kept for 2, 5 and 9 days. the cpc reagent used (0.5% cpc and 0.5% sodium ch ... | 1976 | 773957 |
| low resolution crystal structure of lipase from geotrichum candidum (atcc34614). | lipase from geotrichum candidum (atcc34614) is a glycerol ester hydrolase which has a molecular weight of 55,000 with about 7% carbohydrate, displaying a high affinity for triolein. the enzyme was crystallized from more than 2% protein solution without using any salt or organic solvent. the crystals were cross-linked by soaking in 0.37% glutaraldehyde solution (0.1 m acetate buffer solution, ph 5.6). the structure was determined by x-ray diffraction using the isomorphous replacement technique. t ... | 1979 | 575138 |
| [effect of tweens on the lipase activity of oospora lactis]. | oospora lactis was found to use tweens 20, 40, 60 and 80 as a source of carbon in a nutrient medium for lipase biosynthesis. the highest production of lipase was registered in the medium with tween-80. the lipase activity of the culture increased if even small quantities of tweens 40, 60 and, particularly, 80 (0.05--1.0%) were added to the medium containing cottonseed oil. all the tested tweens stimulating the biosynthesis of lipase were also, at a particular concentration, the inhibitors of thi ... | 1979 | 573368 |
| [physiologic features of geotrichum candidum isolated from the rumen contents of cattle]. | | 1979 | 573363 |
| the in vitro susceptibility of some mycotic agents to a new orally active triazole, itraconazole. | the growth-inhibitory effect of itraconazole against 85 strains of mycotic agents belonging to 37 species was studied. mic values were determined in a microtiter broth dilution method using casitone medium. the widest range of susceptibility was noted for yeasts. more strains of candida albicans, candida, spp. and torulopsis spp. were found to be highly sensitive (mic = 0.22 mg l-1), a single strain of candida tropicalis and rhodotorula glutinis was fully resistant (mic = 200 mg l-1). most isola ... | 1990 | 2170505 |
| [radioimmunological differentiation of yeasts (author's transl)]. | a microtiter solid phase radioimmunoassay is described for the differentiation or microorganisms by using six species of yeasts as a model. rabbits were immunized with homogenized cells of the yeasts. the rabbit sera obtained were absorbed to homologous cells and after elution, the antibodies so obtained were labeled with 125i. cross-reacting antibodies were eliminated by absorption to the corresponding yeast cells (table 1). with this radioimmunoassay it was possible to identify 51 out of 52 st ... | 1978 | 569952 |
| fungal flora of human faeces in psoriasis and atopic dermatitis. | faeces samples taken from 343 patients with psoriasis and 581 patients with atopic dermatitis were subjected to mycological examination. yeasts were detected in 68% of the psoriatics and in 70% of the patients with atopic dermatitis but in only 54% of the controls (n = 50). qualitative analysis revealed a predominance of candida albicans. non-pathogenic yeasts constituted only 1% in each of these groups. of the hyphomycetes, geotrichum candidum occurred in 22% of the psoriatics, in 10% of the at ... | 1990 | 2191222 |
| evaluation of the updated vitek yeast identification data base. | using 398 isolates of yeasts and yeastlike fungi comprising 9 genera and 26 species, as well as the hyphomycete geotrichum candidum and the achlorophyllous alga prototheca wickerhamii, we compared the api 20c yeast identification system with the modified vitek yeast identification system with an expanded data base. we found 11 discrepancies between the two systems: five (1.3%) of the isolates (blastoschizomyces capitatus, 1; candida albicans, 1; hansenula anomala, 1; rhodotorula minuta, 2) had b ... | 1990 | 2229376 |
| [effect of different nitrogen sources on the biosynthesis of lipase by oospora lactis]. | the effect of inorganic and organic nitrogen compounds on the synthesis of biomass and extracellular lipase by oospora lactis was studied. among the inorganic nitrogen sources ammonium sulphate and ammonium secondary phosphate and among the organic nitrogen sources yeast autolysate proved to be most beneficial for the lipase synthesis. lipase activity and biomass accumulation in the medium containing yeast autolysate were greater than in the media containing the above ammonium salts. lipase synt ... | 1978 | 569300 |
| [effect of yeast growth limitation on the respiratory chain, the economic coefficient and the critical oxygen concentration for respiration]. | the effect of the yeast growth limitation by oxygen on the economical coefficient (ec), the operation of the cyanide resistant electron transport pathway (cretp), and the critical for respiration oxygen concentration concentration ([o2]cr) was studied. the operation of cretp was found to differ among various yeasts growing on glucose: it could function during both the exponential phase and limitation of growth (torulopsis candida), or only in the conditions of growth limitation (candida tropical ... | 1978 | 568709 |
| electron microscopic study of negatively stained ribosomal subunits from geotrichum candidum. | | 1978 | 567667 |
| proteolytic activity of geotrichum candidum subjected to the action of nystatin, determined by a personal method. | | 1978 | 565446 |
| [enzyme and respiration activities on the morphological change of geotrichum candidum belonging to sludge fungi (author's transl)]. | | 1977 | 564983 |
| glyceride synthesis by four kinds of microbial lipase. | apart from their usual mechanism of action, lipases from aspergillus niger and rhizopus delemar also catalyzed the synthesis of glycerides from oleic acid and glycerol. lipases from geotrichum candidum and penicillium cyclopium were inactivated by oleic acid, but were stable in the presence of casein, albumin or buffer of appropriate ph. lipases from aspergillus niger and rhizopus delemar synthesized glycerides from, not only fatty acid, but dibasic acids and aromatic acids, making ester bonds o ... | 1977 | 563245 |
| bioconversion of glyceryl trinitrate into mononitrates by geotrichum candidum. | glyceryl trinitrate (gtn) 2 mm was quantitatively converted into its 1 and 2 mononitrate derivatives by geotrichum candidum, with consumption of the nitrite ions produced. the conversion proceeded at a rate independent of the addition of either organic carbon or organic nitrogen sources. eight batches of nitrate ester, which were added every 24 hours, were successfully converted as far as during the bioconversion process gtn concentration did not exceed 2 mm. when those limiting conditions were ... | 1989 | 2515099 |
| experimental studies on the persistence in distilled water of certain conditional pathogenic fungi. | the authors followed up the behaviour in distilled water of 8 fungus strains belonging to four species (candida albicans, c. krusei, geotrichum candidum and rhodotorula) kept at various temperatures (i.e. +4 degrees, +18 degrees and 25 degrees c). the average and the maximum survival periods were found to be as follows: c. albicans--476/1138 days, c. krusei--411/1138 days, g. candidum--127/219 days, and r. rubra--139/226 days. all strains revealed periodically multiplication bursts of variable i ... | 1989 | 2519637 |
| [changes of growth and cell component of geotrichum candidum on the existence of heavy metals and cyan (author's transl)]. | | 1977 | 562432 |
| yeast-suspension as soiling matter in disinfectant testing. | using the kelsey-sykes capacity-test, it was found that a sterile yeast suspension used to simulate 'dirty' conditions, gave an increased effect of chloramine t against the fungi candida albicans, aspergillus fumigatus, geotrichum candidum and penicillium sp. compared with the effect under 'clean' conditions. this effect was not found with the fungus rhodotorula rubra nor on the various bacteria tested. the enhanced effect was found with respect to both chloramine t and chloramine b, but not wit ... | 1989 | 2661973 |
| [in vitro studies on the morphological change of geotrichum candidum belonging to sludge fungi (author's transl)]. | | 1977 | 562431 |
| carbon assimilation and extracellular antigens of some yeast-like fungi. | many yeast-like fungi assimilated n-hexadecane, butylamine and putrescine as sole carbon sources. methanol was not assimilated. this points to a physiological similarity to endomycetous, hydrocarbon-utilizing yeasts. stephanoascus ciferrii assimilated uric acid, adenine and allantoin as sole source of carbon and nitrogen. all strains of geotrichum candidum and many other yeast-like fungi assimilated acetoin and butan-2,3-diol. assimilation tests for adenine, uric acid, allantoin, acetoin and but ... | 1989 | 2742372 |
| rapid identification of medically important yeasts by electrophoretic protein patterns. | a simple electrophoretic method for yeast identification was evaluated. whole cells were extracted by sds and the protein profiles obtained in sds-page after coomassie blue staining were compared for 52 strains from 9 species of yeast or yeast-like fungi commonly isolated from man (candida albicans, c. glabrata, c. guilliermondii, c. krusei, c. parapsilosis, c. pseudotropicalis, c. tropicalis, geotrichum candidum, saccharomyces cerevisiae). the corresponding patterns showed 30 to 45 polypeptides ... | 1989 | 2744423 |
| the identification of rear profile of monomer ribosomes from geotrichum candidum with electron microscopy. | | 1977 | 561824 |
| isolation of keratinophilic fungi from floor dust in arab elementary and preparatory schools in the west bank of jordan. | floor dust collected from classrooms of thirty three elementary schools (16 for girls, and 17 for boys) (children aged 6-11), and twenty four preparatory schools (13 for girls, and 11 for boys) (children aged 12-14) was studied for the occurrence of keratinophilic fungi with respect to human presence and age of children. tichophyton mentagrophytes was present in 15.4% of the preparatory schools for girls, in 12.5% of elementary schools for girls, and in 11.8% of elementary schools for boys. it w ... | 1989 | 2770839 |
| oral geotrichum candidum infection associated with hiv infection. a case report. | infections with geotrichum species, although rare, are sometimes seen in immunocompromised hosts. we report a case of oral geotrichosis in a patient seropositive for human immunodeficiency virus who had erythematous mandibular and maxillary gingiva but was otherwise free of any active systemic disease. geotrichum candidum was shown by both culture and histopathology to be present in the lesion and was deduced to be the causative organism. the patient responded well to several weeks of treatment ... | 1992 | 1437044 |
| [experimental studies relative to the persistence of several facultative pathogenic fungi in river water]. | under laboratory conditions survival time of 3 fungi species (candida albicans, geotrichum candidum, rhodotorula rubra) in river water of various quality and temperature has been studied. the mean (and the maximum) survival time was as follows: 82 (240) d for c. albicans, 210 (578) d for g. candidum and 333 (606) d for r. rubra. differences concerning survival time were recorded in dependence on temperature and quality of water. all the 3 strains were multiplied periodically. variants of culture ... | 1988 | 3066060 |
| beta-glucosidases from cellulolytic fungi aspergillus terreus, geotrichum candidum, and trichoderma longibrachiatum as typical glycosidases. | by ethanol precipitation (v/v) and chromatography on sephadex sp, deae (or deae-cellulose), and g-200 beta-glucosidases (ec 3.2.1.21) from the culture filtrates of cellulolytic fungi aspergillus terreus, geotrichum candidum, and trichoderma longibrachiatum grown on the medium with cellulose containing materials were isolated. the enzymes were homogenous as shown by different techniques. the substrate specificities of the obtained enzymes were studied. beta-glucosidases had higher affinity for p- ... | 1987 | 3111495 |
| homology-derived three-dimensional structure prediction of candida cylindracea lipase. | we propose a structural model of candida cylindracea lipase (ccl) based on the reported x-ray structure of the highly homologous geotrichum candidum lipase (gcl). the network of interactions around the active site, the salt and disulfide bridge pattern is conserved in the proposed structure. functional, structural and evolutionary aspects of the peculiar usage of ctg codons by c. cylindracea atcc 14830 are discussed. | 1992 | 1420342 |
| dermatomycoses among industrial workers in cross river state, nigeria. | a survey of dermatomycoses was carried out amongst industrial workers in three different factories during the period 1987-1988. a total of 194 workers were screened, out of which 54 proved to be mycologically positive by microscopy and/or culture. incidence was apparently highest amongst workers in a cement factory (calcemco, 33.3%) followed by those in a wood factory (seromwood, 30.8%) and a rubber factory (crel, 26.2%). pityriasis versicolor was the predominant clinical type of dermatomycosis, ... | 1992 | 1302813 |
| fungal infections in cancer patients: an escalating problem. | major advances in anticancer treatment have contributed to an increased frequency of severe fungal infections in patients with neoplastic diseases. neutropenia remains the most important among the predisposing factors related to the malignancy or its treatment. most fungal infections are caused by the commonly recognized opportunistic fungi candida spp and aspergillus spp, and the pathogenic fungi cryptococcus neoformans, histoplasma capsulatum, coccidiodes immitis, and less often by blastomyces ... | 1992 | 1525339 |
| pancreatic lipases: evolutionary intermediates in a positional change of catalytic carboxylates? | comparison of the fold of lipases from geotrichum candidum and from human pancreas identified a high degree of similarity which was not expected on the basis of their amino acid sequences. although both enzymes utilize a serine protease-like catalytic triad, they differ in the topological position of the acid. we speculate that these proteins are evolutionarily related and that the pancreatic lipase is an evolutionary intermediate in the pathway of migration of the catalytic acid to a new positi ... | 1992 | 1537823 |
| use of specific polyclonal antibodies to detect heterogeneous lipases from geotrichum candidum. | geotrichum candidum cmicc 335426 was previously shown to produce two lipases termed lipase a and lipase b, lipase b being highly specific for hydrolysis of esters of cis-delta 9 fatty acids. we now describe the isolation of polyclonal antibodies specific for lipase a and lipase b. these antibodies were used in western blotting techniques to detect the appearance of the lipases during the course of the fermentation of g. candidum cmicc 335426. a and b were found to be produced simultaneously in t ... | 1992 | 1643106 |
| purification and properties of extracellular polysaccharide (eps) antigens produced by different mould species. | extracellular polysaccharide (eps) antigens produced by different mould species were purified and partially characterized. purification included (nh4)2so4 treatment, sepharose cl-4b column chromatography and con a-sepharose chromatography. the eps of penicillium digitatum, mucor racemosus and cladosporium cladosporioides showed high antigenic capacities. immunologically the eps were partially genus-specific, but cross-reactivity was observed. the eps antigens produced by species of penicillium, ... | 1987 | 3571038 |
| substrate specificities of lipases a and b from geotrichum candidum cmicc 335426. | the mould geotrichum candidum produces extracellular lipases with different substrate specificities according to strains. we purified two lipases - termed lipase a and lipase b - from geotrichum candidum cmicc 335426. the specificity of the two lipases was investigated using hydrolysis assays on emulsions of pure acylglycerols and a wide range of fatty acid esters. lipase b was very highly specific for hydrolysis of esters of cis-delta 9-fatty acids. lipase a did not show such strict specificity ... | 1992 | 1730047 |
| geotrichum candidum produces several lipases with markedly different substrate specificities. | we have purified and examined the substrate specificity of four lipases from two strains of the mould geotrichum candidum, atcc 34614 and cmicc 335426. we have designated the lipases i and ii (atcc 34614), and a and b (cmicc 335426). the enzymes are monomeric and have similar molecular masses and pi. thus, lipases i and ii have native molecular masses of 50.1 kda and 55.5 kda, and pi of 4.61 and 4.47, respectively. lipases a and b are very similar to lipases i and ii with native molecular masses ... | 1991 | 1761048 |
| geotrichum candidum nrrl y-553 lipase: purification, characterization and fatty acid specificity. | lipases from geotrichum candidum nrrl y-553 are of interest because of their unique specificity for cis-9-unsaturated fatty acids relative to both stearic and palmitic acids. the lipases were partially purified by chromatography on octyl sepharose, ag mp-1 macroporous anion exchanger, and chromatofocusing resin. the preparation was found to contain multiple, glycosylated lipases varying slightly in pi (pi 4.88, 4.78, 4.65, 4.57 and 4.52) as judged by both activity and silver staining. the molecu ... | 1991 | 1795605 |
| characterization of two beta-tubulin genes from geotrichum candidum. | the beta-tubulin genes g beta 1 and g beta 2 from the phytopathogenic hemiascomycete geotrichum candidum were found to be highly diverged in amino acid sequence from those of other filamentous fungi. g beta 1 and g beta 2 were also divergent from each other, with the coding regions sharing only 66% nucleotide sequence homology and 64% amino acid identity. however, the proteins shared 82% similarity and only 25 of the 161 non-identical amino acid substitutions were non-conservative. the organizat ... | 1991 | 1836049 |
| indirect immunofluorescence staining and crossed immunoelectrophoresis for differentiation of candida albicans and geotrichum candidum. | rabbit hyperimmune antisera were used for differential diagnosis of candida albicans and geotrichum candidum in murine and bovine tissues by indirect immunofluorescence (iif) staining. although the antisera did not detect common antigens by crossed immunoelectrophoresis (xie) a marked cross-reactivity of fungal cells was observed in the iif assay. following liquid phase absorption with heterologous antigenic preparations the hyperimmune antisera were rendered specific for each species thus makin ... | 1990 | 2103589 |
| does sequence similarity of human choline esterase, torpedo acetylcholine esterase and geotrichum candidum lipase reveal the active site serine residue? | | 1990 | 2115773 |
| [studies of compounds with potential antiparasitic action. viii. new oxime ethers in the 1-thiochromone system]. | the 50% antitrichomonal concentration of 7 examined ethers (t. vaginalis strain n. 1/86, roiron medium, safranine staining, 30 min.) was estimated by means of dose-response curve. the minimal mycostatic concentration (candida albicans l-45, geotrichum candidum; 3% sabouraud agar, 37 degrees c, 24 hr) was calculated with the aid of regression equation. all examined ethers show in vitro a marked antitrichomonal effect comparable with activity of ornidazole or phenol. mycostatic activity of new com ... | 1990 | 2131701 |
| characterization of siderophore-mediated iron transport in geotrichum candidum, a non-siderophore producer. | geotrichum candidum is capable of utilizing iron from hydroxamate siderophores of different structural classes. the relative rates of iron transport for ferrichrome, ferrichrysin, ferrioxamine b, fusigen, ferrichrome a, rhodotorulic acid, coprogen b, dimerium acid and ferrirhodin were 100%, 98%, 74%, 59%, 49%, 35%, 24%, 12% and 11% respectively. ferrichrome, ferrichrysine and ferrichrome a inhibited [59fe]ferrioxamine-b-mediated iron transport by 71%, 68% and 28% respectively when added at equim ... | 1990 | 2143917 |
| growth of opportunistic yeasts on vitamin-free solid medium. | the growth of some opportunistic yeasts (candida albicans, cryptococcus neoformans, torulopsis glabrata, rhodotorula rubra, geotrichum candidum, trichosporon cutaneum, saccharomyces cerevisiae) was essayed in a solid minimal synthetic medium without vitamins. from the preliminary results some species (candida albicans, cryptococcus neoformans, rhodotorula rubra, geotrichum candidum and saccharomyces cerevisiae) seem to be autotrophic regarding the growth factor considered indispensable for their ... | 1990 | 2191196 |
| purification and characterization of two distinct lipases from geotrichum candidum. | lipase, an enzyme that hydrolyzes triacylglycerol, has been purified and characterized. the purification procedure includes ethanol precipitation and chromatographies on sephacryl-200 hr, high resolution anion-exchange (mono q) and polybuffer exchanger 94. with this procedure, two forms of lipases from geotrichum candidum were obtained. lipase i (main enzyme) and lipase ii (minor enzyme) were purified 35-fold with a 62% recovery in activity and 94-fold with a 18% recovery in activity, respective ... | 1990 | 2340308 |
| separation and characterization of two molecular forms of geotrichum candidum lipase. | southern blot analysis of the geotrichum candidum genome with a cloned lipase cdna as the probe indicated the existence of two genes on the chromosome of the fungus which are homologous to the cdna. as expected, two forms of lipase (lipases i and ii) were actually isolated by hydrophobic interaction chromatography after a multistep procedure including ammonium sulfate fractionation, anion exchange chromatography, and gel filtration of the culture filtrate. lipase i, the first eluted fraction, wa ... | 1990 | 2341377 |
| disseminated geotrichosis in two dogs. | disseminated geotrichosis was diagnosed at necropsy of 2 unrelated dogs from the same household. clinical signs of disease included fever, coughing, anorexia, listlessness, polydipsia, and dyspnea. the duration of clinical illness was less than 2 weeks. pathologic findings in both dogs were similar and consisted of pyogranulomatous pneumonia, hepatitis, and nephritis. geotrichum candidum was identified by fluorescent antibody technique. | 1990 | 2391272 |
| [esophageal ulcer caused by geotrichum candidum in a case of aids]. | isolate esophageal ulcer caused by geotrichum candidum in a case of aids. we report the case of a 29-year-old white man, aids affected, presented with odynophagia, dysphagia and chest pain. x-ray and endoscopic study revealed an isolated esophageal ulcer whose culture demonstrated to be caused by geotrichum candidum. this etiologic agent was not previously described in this kind of lesion. | 1990 | 2392339 |
| cdna cloning and characterization of geotrichum candidum lipase ii. | geotrichum candidum produces two extracellular lipases, i and ii. a lipase ii cdna clone was isolated from a cdna library by colony hybridization using the 32p-labeled fragment of lipase i cdna isolated previously. the nucleotide sequence of lipase ii cdna determined by the dideoxy chain terminating method includes the n- and c-terminal amino acid sequences of lipase ii, and the overall amino acid composition deduced from the cdna coincides with that deduced on amino acid analysis of this protei ... | 1990 | 2398037 |
| cdna molecular cloning of geotrichum candidum lipase. | the cdna clone of geotrichum candidum (geo.) lipase was isolated from the geo. cdna library by colony hybridization using 32p-labeled oligonucleotides corresponding to a partial amino acid sequence of this enzyme. the nucleotide sequence of the cdna determined by the dideoxy chain terminating method included some partial amino acid sequences determined by edman degradation, and the overall amino acid composition deduced from the cdna coincided with that from amino acid analysis of this protein. ... | 1989 | 2481674 |
| [a case of high intestinal obstruction caused by mycotic bezoar of the duodenum]. | a case of the mycotic bezoar in the female patient with gastric hypersecretion is reported. the symptoms of the high intestinal obstruction accompanied underlying disease. bezoar formed of geotrichum candidum was fragmentated with biopsical forceps of "alligator" type. then, natamycin was administered for 5 weeks. the patients recovered completely. | 1989 | 2626355 |
| enzyme activity electrophoresis and rocket immunoelectrophoresis for the qualitative and quantitative analysis of geotrichum candidum lipase activity. | the development and application of a rocket immunoelectrophoretic and an enzyme activity electrophoretic assay for the qualitative analysis of geotrichum candidum lipase activity is presented. the sensitivities of the four assays were (in arbitrary units): enzyme activity electrophoresis, 1-0.5; rocket immunoelectrophoresis, 0.5-0.2; radial diffusion, 1; titrimetry, 1. the electrophoretic methods made it possible to distinguish between high and low molecular weight forms of the g. candidum lipas ... | 1989 | 2714239 |
| new spectrum of fungal infections in patients with cancer. | we report on 44 cancer patients who had serious infections with unusual fungal pathogens and who were cared for at our cancer center between 1974 and 1986. twelve different fungal species accounted for these infections, including trichosporon beigelii, fusarium species, geotrichum candidum, curvularia species, drechslera species, penicillium species (but not penicillium marneffei), rhodotorula rubra, pseudallescheria boydii, pichia farinosa, torulopsis pintolopesii, saccharomyces cerevisiae, and ... | 1989 | 2749101 |
| seminal vesicle and coagulating gland growth induced by intraperitoneal inoculation of fungi in mice. | the effect of fungi on the growth of body organs in mice was investigated. single, intraperitoneal injections of yeasts (cryptococcus albidus, saccharomyces cerevisiae, schizosaccharomyces octosporus) or molds (aspergillus niger, geotrichum candidum, mucor haemalis) induced an increase in the mass of seminal vesicles and coagulating glands independent of whole body weight changes in mice. | 1989 | 2919824 |
| geotrichum candidum--an opportunistic agent of mycotic diseases. | | 1988 | 3173427 |
| production, purification and properties of geotrichum candidum polygalacturonase: regulation of production by pyruvate. | | 1988 | 3240928 |
| [induction of cellulases and xylanases in aureobasidium pullulans]. | the specificity or induction of wood-degrading enzymes using series of mono, di or polysaccharides as carbon source for the yeasts geotrichum candidum and trychosporon penicillatum and the yeast-like organism aureobadidium pullulans were studied. the strain a. pullulans was the only one that when xylan or "steam exploded wood" were used as carbon source all the enzymes tested were found. this strain was unable to grow on carboxymethyl or avicel cellulose. d-xylose was the nutritional inducer of ... | 1988 | 3268297 |
| allergenic cross-reactivity of yeasts. | yeast allergen extracts of candida albicans, c. pseudotropicalis, c. krusei, c. parapsilosis, c. tropicalis, c. guilliermondi, c. humicola, c. norwegica, c. utilis, cryptococcus albidus, geotrichum candidum, pityrosporon pachydermatis, p. ovale, rhodotorula minuta, r. rubra, saccharomyces cerevisiae, torulopsis glabrata and trichosporon cutaneum were investigated regarding their common allergenic properties. the enzyme immunoassay (eia) using rabbit anti-candida albicans antiserum showed remarka ... | 1988 | 3287997 |
| disseminated geotrichum candidum infection. | the pathogenicity of the fungus geotrichum candidum in humans has not been clearly defined. a patient with acute leukemia who developed a fatal disseminated g. candidum infection while neutropenic is described. at autopsy examination, this patient was misdiagnosed on the basis of histopathology as having disseminated candidiasis until g. candidum was isolated from postmortem culture specimens. the emergence of this organism as an occasional pathogen in leukemic patients is of interest and illust ... | 1987 | 3477570 |
| evaluation of the new mycotube test-kit for yeast identification. | the performance of a modified mycotube test for the identification of yeasts was evaluated using standard biochemical tests as reference. one hundred and eighty strains belonging to 12 medically important species (15 strains each) were tested. the overall rate of identification was 72%, and rose to 87.8% when macroscopic and microscopic morphologic features were also considered; a 100% rate was obtained with candida albicans, candida krusei, candida parapsilosis, candida pseudotropicalis, candid ... | 1985 | 3886377 |
| growth of bacteria on chitin, fungal cell walls and fungal biomass, and the effect of extracellular enzymes produced by these cultures on the antifungal activity of amphotericin b. | vibrio alginolyticus, streptomyces griseus, arthrobacter g12, bacillus sp. and cytophaga sp. were grown on solid and liquid media containing soluble and insoluble carbon sources. arthrobacter g12, bacillus sp. and cytophaga sp. grew well on media which contained fungal cell walls or fungal biomass as the main carbon source. all bacteria produced extracellular proteases and all bacteria except arthrobacter g12 produced extracellular chitinases. growth of cytophaga sp. on colloidal chitin was para ... | 1985 | 3929028 |
| irritable bowel syndrome and hypersensitivity to food. | food hypersensitivity as a cause of irritable bowel syndrome was investigated by means of exclusion diet and blind provocation. twelve atopic and twelve non-atopic individuals entered into the study. skin prick testing with 20 foods and food additives and rast specific for food only, were done in all cases. serum ige level was also measured. in 14 patients one or several food or additives were shown to induce the typical symptoms of ibs. in at least nine cases of atopy, an ige-mediated mechanism ... | 1985 | 4014782 |
| inhibition of lipases by proteins. a kinetic study with dicaprin monolayers. | we report further investigations on protein inhibition of pancreatic and microbial lipases carried out with the monolayer technique. when beta-lactoglobulin a, melittin, serum albumin, myoglobin, and a protein inhibiting lipase from soybean were preincubated with a dicaprin film at a surface pressure of 35 dynes/cm, no activity was detected with horse pancreatic or rhizopus delemar lipases. by contrast, rhizopus arrhizus and geotrichum candidum lipase activities were not impaired under the same ... | 1985 | 4038705 |
| association of anurans with pathogenic fungi. | in a study of 450 amazonian anurans, we isolated yeasts and yeast-like fungi from 54 animals (bufo granulosus, b. marinus, dendrophrynyscus sp., hyla geographica, h. lanciformes, ololygon rubra, adenomera hylaedactyla, eleutherodactylus fenestratus, leptodactylus fuscus, l. ocellatus, l. pentadactylus). the internal organs of these animals did not show any macroscopic anomaly nor histopathology. we recovered 105 fungal isolates from the anuran liver, lung, kidney, spleen, heart and gonad. the is ... | 1985 | 4069190 |
| lysosome-like particles in geotrichum candidum: a cytochemical study. | | 1971 | 4110595 |
| double-membraned vesicles in geotrichum candidum. | | 1973 | 4121695 |