| isolation and characterization of the main toxic fraction from the venom of the false horned viper (pseudocerastes fieldi). | the venom of pseudocerastes fieldi was subjected to gel filtration on sephadex g-75. most of the protein and lethality of the venom were eluted in a major symmetrical peak (c). the lethality of this peak is confined to a basic protein fraction, cb (pi greater than 9.5) separable by deae-cellulose chromatography. two proteins with molecular sizes close to 16,000 daltons were isolated from this fraction by preparative acidic gel electrophoresis in the presence of triton x-100. one of the proteins ... | 1982 | 7179294 |
| venomous snakes of israel: ecology and snakebite. | ten species of venomous snakes belonging to three families occur in israel and in jordan, some of which may pose a serious threat to humans. specific, local antivenins are available against only two of the species, while against others regional or european preparations are used. it is suggested that in addition to the monospecific anti-vipera palaestinae, a polyspecific antivenin be prepared against the clinically most important venomous snakes of the region, namely, echis coloratus, pseudoceras ... | 1998 | 10444959 |
| mode of neuromuscular blocking action of a toxic phospholipase a2 from pseudocerastes fieldi (field's horned viper) snake venom. | the effects of a toxic phospholipase a2 (fr.cb) isolated from the venom of pseudocerastes fieldi were studied on the chick biventer cervicis muscle and the mouse phrenic nerve--diaphragm preparations. in the chick muscle, fr.cb (10 micrograms/ml) caused complete neuromuscular blockade without producing contracture or affecting the response of the muscle to acetylcholine. in the mouse diaphragm, fr.cb blocked the indirectly elicited contraction without affecting that evoked directly. in a low cal ... | 1983 | 6623493 |
| the neurotoxic complex from the venom of pseudocerastes fieldi. contribution of the nontoxic subunit. | the neurotoxic complex (cb) from the venom of pseudocerastes fieldi consists of an acidic non-toxic subunit (cbi) and a basic toxic one (cbii). the complex is only partially dissociated by salt-gradient chromatography; the two components are completely separable in the presence of urea. chromatofocusing of cbi resulted in two protein peaks, both of which potentiated toxicity of cbii. cbi inhibits hemolysis induced by cbii, but not the phospholipase a2 activity of cbii. cbi reveals phospholipase ... | 1985 | 4084321 |
| interactions of the neurotoxic complex from the venom of the false horned viper (pseudocerastes fieldi) with rat striatal synaptosomes. | the very high lethal potency of the neurotoxic complex (cb) from the venom of pseudocerastes fieldi following direct administration into the lateral ventricle of the brain, as compared with potency following i.v. administration, suggests that the toxin acts on the central nervous system. rat striatal synaptosomes were selected to study interactions of the toxin with nerve endings. cbii, the toxic phospholipase a2 component of the toxin, as well as the reconstituted complex (cbi + cbii), inhibite ... | 1987 | 3590210 |
| extreme venom variation in middle eastern vipers: a proteomics comparison of eristicophis macmahonii, pseudocerastes fieldi and pseudocerastes persicus. | venoms of the viperid sister genera eristicophis and pseudocerastes are poorly studied despite their anecdotal reputation for producing severe or even lethal envenomations. this is due in part to the remote and politically unstable regions that they occupy. all species contained are sit and wait ambush feeders. thus, this study examined their venoms through proteomics techniques in order to establish if this feeding ecology, and putatively low levels of gene flow, have resulted in significant va ... | 2015 | 25241240 |
| antigenic cross-reactivity and species-specific identification of pseudocerastes persicus fieldi snake venom. | in the present study, we recognized progressively high immunological cross-reactivity between pseudocerastes persicus fieldi (pf) venom and six other medically important egyptian snake venoms belonging to families viperidae and elapidae. antibodies with a range of bonding strengths were shown to be involved in such cross-reactivity. two strategies have been tried to access specificity; (i) using affinity purified species-specific anti-pf antivenom antibodies, (ii) conducting the assay in the pre ... | 2016 | 27319296 |
| comparison of venoms from two subspecies of the false horned viper (pseudocerastes persicus). | gel-isoelectric focusing of pseudocerastes persicus fieldi venom shows a simple pattern with very few protein bands, while the venom of p. p. persicus reveals close to 30 protein bands. molecular sieve chromatography of p. p. persicus venom shows a typical elution profile of a viperid snake, with hemorrhagic activity and l-amino acid oxidase activity confined to the high molecular weight peak of proteins. in the venom of p. p. fieldi, which has no hemorrhagin or l-amino acid oxidase, the high mo ... | 1986 | 3775788 |
| amino acid sequences of a heterodimeric neurotoxin from the venom of the false horned viper (pseudocerastes fieldi). | the main toxic component of the venom of the false horned viper, pseudocerastes fieldi, is a heterodimeric neurotoxin composed of a basic subunit, cb ii, and one of two acidic subunits, either cb i alpha or cb i beta. the nontoxic acidic subunit increases the toxicity of the basic subunit. both subunits have phospholipase a2 (pla2) amino acid sequences. cb i alpha and cb i beta themselves are inactive towards phosphatidylcholine and when complexed with cb ii promote a delay in the onset of phosp ... | 1995 | 8588211 |
| survey of the reptilian fauna of the kingdom of saudi arabia. vi. the snake fauna of turaif region. | a collection of snakes in turaif region, kingdom of saudi arabia, an area that has been poorly documented for reptiles, consists of 28 specimens representing 11 species belonging to 4 families (colubridae, elapidae, viperidae and atractaspididae). this study presents the first comprehensive inventory of the herpetofauna of the turaif province of saudi arabia. co-ordinates: latitude, longitude and altitude, of the collected specimens were mapped using gps. three of the snake specieslytorhynchus d ... | 2017 | 28490966 |
| cross neutralization of dangerous snake venoms from africa and the middle east using the vacsera polyvalent antivenom. egyptian organization for biological products & vaccines. | this study was performed to assess the ability of polyvalent snake venom anti-serum, produced by the egyptian organization for biological products & vaccines (vacsera), to neutralize several toxic activities of snake venoms, not only of those included in the antivenom mixture, but also some additional venoms of snakes from egyptian, african, and middle eastern habitats. in general, the results revealed that polyvalent snake venom anti-serum from vacsera is highly effective in neutralizing egypti ... | 2002 | 12503876 |
| epidemiology of venomous and semi-venomous snakebites (ophidia: viperidae, colubridae) in the kashan city of the isfahan province in central iran. | information on the epidemiology of venomous snake species responsible for envenomation to humans in iran has not been well documented. in the kashan city, venomous snakebite remains a recurring medical problem. information providing the correct identification of snake species responsible for envenomation in this geographic region would be useful to regional medical clinics and personnel for the effective and optimal management of the patients. | 2014 | 24672563 |