| genetic characterization of a new astrovirus detected in dogs suffering from diarrhoea. | astroviruses have been described in several animals species frequently associated with diarrhoea, especially in young animals. in dogs, astrovirus-like particles have been observed sporadically and very little is known about their epidemiology and characteristics. in this paper, we describe the detection of astrovirus-like particles in symptomatic puppies. furthermore, for the first time in this species, the presumptive identification made by electron microscopy was confirmed by genetic analysis ... | 2009 | 19477085 |
| isolation and characterization of canine astrovirus in china. | in this study, we first investigated the prevalence of astrovirus in stools of dogs with and without diarrhea in shanghai, china. of all the specimens, 22 (12.02%) from the 183 dogs with diarrhea and none (0%) from the 138 healthy controls were positive for astrovirus. furthermore, we cloned partial sequences of orf1b (442 bp) and the entire sequences of orf2 (2475 bp). phylogenetic analysis showed that the new isolates were belonged to genus mamastrovirus and most closely clustered with the ita ... | 2011 | 21604183 |
| genome analysis of canine astroviruses reveals genetic heterogeneity and suggests possible inter-species transmission. | canine astrovirus rna was detected in the stools of 17/63 (26.9%) samples, using either a broadly reactive consensus rt-pcr for astroviruses or random rt-pcr coupled with massive deep sequencing. the complete or nearly complete genome sequence of five canine astroviruses was reconstructed that allowed mapping the genome organization and to investigate the genetic diversity of these viruses. the genome was about 6.6kb in length and contained three open reading frames (orfs) flanked by a 5' utr, a ... | 2017 | 27965150 |
| detection and genetic characterization of canine astroviruses in pet dogs in guangxi, china. | astroviruses (astvs) have been reported to infect and cause gastroenteritis in most animal species. human astvs were regarded the causative agent of viral diarrhea in children. in dogs, little is known about the epidemiology and clinical significance of astv infection. | 2017 | 28814340 |
| prevalence and risk factors of astrovirus infection in puppies from french breeding kennels. | aiming at determining the prevalence and the risk factors associated to astrovirus infection in puppy, fecal samples were collected in 316 puppies (age from 5 to 14 weeks of age) from 33 french breeding kennels. data were registered for each puppy, including age, breed, gender, origin of the dog, and feces quality. the samples were tested by specific rt-pcr for the presence of canine astrovirus. astroviruses were identified in 20.9% (66/316) of the puppies and in 42% (14/33) of the breeding kenn ... | 2012 | 22304762 |
| complete genome sequence of canine astrovirus with molecular and epidemiological characterisation of uk strains. | astroviruses are a common cause of gastroenteritis in children worldwide. these viruses can also cause infection in a range of domestic and wild animal species. canine astrovirus (caastv) was first identified in the usa, and has since been reported in dogs from europe, the far east and south america. we sought to determine whether caastv is circulating in the uk dog population, and to characterise any identified strains. stool samples were collected from pet dogs in the uk with and without gastr ... | 2015 | 25818578 |
| detection of canine astrovirus in dogs with diarrhea in japan. | canine astrovirus (castv) is the causative agent of gastroenteritis in dogs. we collected rectal swabs from dogs with or without diarrhea symptoms in japan and examined the feces for the presence of castv by rt-pcr with primers based on a conserved region of the orf1b gene. the orf1b gene of castv was not detected in the 42 dogs without clinical illness but was present in three pups out of the 31 dogs with diarrhea symptoms. based on the full-length capsid protein, the castv ku-d4-12 strain that ... | 2015 | 25824600 |
| sewage surveillance reveals the presence of canine gvii norovirus and canine astrovirus in uruguay. | canine norovirus (nov) and astrovirus (astv) were studied in 20 domestic sewage samples collected in two cities in uruguay. four samples were characterized as canine astv after phylogenetic analysis clustering with strains detected in italy and brazil in 2008 and 2012, respectively. one sample was characterized as canine nov and clustered with a strain detected in hong kong and recently classified as gvii. this study shows the occurrence of a canine nov gvii strain for the first time in the amer ... | 2015 | 26280526 |
| characterisation of the canine faecal virome in healthy dogs and dogs with acute diarrhoea using shotgun metagenomics. | the virome has been increasingly investigated in numerous animal species and in different sites of the body, facilitating the identification and discovery of a variety of viruses. in spite of this, the faecal virome of healthy dogs has not been investigated. in this study we describe the faecal virome of healthy dogs and dogs with acute diarrhoea in australia, using a shotgun metagenomic approach. viral sequences from a range of different virus families, including both rna and dna families, and ... | 2017 | 28570584 |
| prevalence of classic, mlb-clade and va-clade astroviruses in kenya and the gambia. | infectious diarrhea leads to significant mortality in children, with 40 % of these deaths occurring in africa. classic human astroviruses are a well-established etiology of diarrhea. in recent years, seven novel astroviruses have been discovered (mlb1, mlb2, mlb3, va1/hmo-c, va2/hmo-b, va3/hmo-a, va4); however, there have been few studies on their prevalence or potential association with diarrhea. | 2015 | 25975198 |
| Enteric disease in dogs naturally infected by a novel canine astrovirus. | Infection by a novel canine astrovirus was associated with gastroenteritis in two dogs. The virus displayed 70.3-73.9% aa identity to other canine astroviruses in the full-length capsid. Specific antibodies were detected in the convalescent sera of the dogs, indicating seroconversion. Also, the virus appeared weakly related antigenically to the prototype canine astrovirus isolate ITA/2008/Bari. | 2011 | 22189118 |