| evidence for a negative membrane potential and for movement of c1- against its electrochemical gradient in the ascomycete neocosmospora vasinfecta. | the iodides of three lipid-soluble cations (dibenzyldimethylammonium; tribenzylmethylammonium, tbma+; ethyldimethylbenzylammonium) were synthesized by the reaction of 14c-labeled methyl or 14c-labeled ethyl iodide with the appropriate secondary of tertiary amine and used in an attempt to measure the transmembrane electrical potential difference in neocosmospora. only mycelium containing high levels of na+ accumulated measureable amounts of these cations and only above ph 6. uptake was reduced in ... | 1976 | 11206 |
| halide uptake by the filamentous ascomycete neocosmospora vasinfecta. | the uptake of cl minus by the ascomycetes neocosmospora vasinfecta was investigated. intramycelial concentrations of more than 55 mm (890-fold the external concentration) were reached. accumulation was as inorganic cl minus and nystatin induced total loss of mycelial cl minus without extensive protein loss, implying that cl minus retention was not due to binding to macromolecules. cl minus transport was largely unidirectional with efflux being low under all conditions. uptake was temperature dep ... | 1975 | 234943 |
| the development of an increased rate of cl- uptake in the ascomycete neocosmospora vasinfecta. | freshly harvested mycelium of the filamentous ascomycete neocosmospora vasinfecta accumulated c1- against a concentration gradient by a process probably requiring the expenditure of metabolic energy. when mycelium, washed free of growth medium, was incubated in deionized water or tris (hydroxymethyl) aminomethane sulfate at ph 7.5 for 4 h and then transferred to k36c1 solutions, the c1- uptake rate was, on the average, 3.77 +/- 0.26 (+/-se, n = 20) times the uptake rate exhibited by freshly harv ... | 1975 | 240498 |
| potassium fluxes in neocosmospora vasinfecta. | the unidirectional k+ fluxes across the mycelial surface of neocosmospora vasinfecta were determined using 42k. influx was mediated by at least two kinetically distinct systems, one having an apparent km of 6-5 mu-equiv. k+/l and the other of about 1-0 m-equiv. k+/l. the vmax for both systems was in the range 18 to 22 mu-equiv. k+/100 mg mycelial dry matter/h (1-0 to 1-2 m-equiv. k+/l cell-water/min). influx was strongly inhibited by 2,4-dinitrophenol, sodium azide, sodium arsenate and anaerobio ... | 1975 | 1194894 |
| influence of exogenous sugars and polyols on c1-influx and efflux by the ascomycete neocosmospora vasinfecta. | glucose and other transportable sugars and polyols inhibited cl- influx very soon after addition to mycelium in the process of cl- accumulation. under the usual experimental conditions (0.1 mm kcl, glucose greater than or equal to 2 mm) the mean percentage of inhibition of cl- influx by glucose was 54.1 +/- 8.0 (+/- standard error; n = 26). transport of the exogenous carbohydrate was necessary for inhibition of cl- influx. thus, the estimated ki for glucose inhibition of cl- influx (28 mum) was ... | 1976 | 1262314 |
| [fungi isolated from diseased medicinal plants]. | one hundred and forty-four fungal isolates were obtained from diseased paeonia albiflora pall. var. trichocarpa bung., astragalus membranaceus bung., lithospermum erythrorhizon sieb. et zucc., ledebouriella seseloides wolff and bupleurum falcatum l. which were collected in the test field of tsukuba medicinal plant research station, national institute of hygienic sciences. most of them were identified into 15 genera containing 8 species. fungal species presumed to be pathogens of the host plants ... | 1992 | 1364438 |
| potassium transport in non-growing mycelium of neocosmospora vasinfecta. | | 1969 | 5370297 |
| the assimilation of bicarbonate by neocosmospora vasinfecta. | | 1969 | 5786778 |
| phylogenetic relationships of the soybean sudden death syndrome pathogen fusarium solani f. sp. phaseoli inferred from rdna sequence data and pcr primers for its identification. | phylogenetic relationships of several species within the fusarium solani-complex were investigated using characters from the nuclear ribosomal dna. genetic variation within 24 isolates, including 5 soybean sudden death syndrome (sds) strains, was assessed using rdna sequence data and restriction fragment length polymorphic markers. by these techniques, the causal agent of soybean sds was identified as f. solani f. sp. phaseoli. in separate cladistic analyses, plectosphaerella cucumerina and nect ... | 1995 | 7579615 |
| leg granuloma due to neocosmospora vasinfecta in a renal graft recipient. | | 1993 | 8516903 |
| specific detection of fusarium species in blood and tissues by a pcr technique. | fusarium species are opportunistic nosocomial pathogens that often cause fatal invasive mycoses. we designed a primer pair that amplifies by pcr a fragment of a gene coding for the rrna of fusarium species. the dnas of the main fusarium species and neocosmospora vasinfecta but not the dnas from 11 medically important fungi were amplified by these primers. the lower limit of detection of the pcr system was 10 fg of fusarium solani dna by ethidium bromide staining. to test the ability of this pcr ... | 1999 | 10405380 |
| osteoarthritis caused by neocosmospora vasinfecta. | we report the case of a patient who developed an ankle osteoarthritis due to the ascomycete neocosmospora vasinfecta, following accidental multiple trauma to his legs in whilst in africa. antifungal susceptibility testing was performed. despite a low amphotericin b minimal inhibitory concentration, parenteral antifungal therapy failed and amputation was required to resolve the osteoarthritis. possible reasons for the failure of this antifungal treatment are examined. | 1999 | 10421854 |
| disseminated neocosmospora vasinfecta infection in a patient with acute nonlymphocytic leukemia. | we report neocosmospora vasinfecta infection following chemotherapy for acute nonlymphocytic leukemia. n. vasinfecta, a plant pathogen, was identified by culture and genetic sequencing. susceptibility testing revealed in vitro resistance for common antifungals. | 2001 | 11266308 |
| a novel saponin hydrolase from neocosmospora vasinfecta var. vasinfecta. | we isolated a soybean saponin hydrolase from neocosmospora vasinfecta var. vasinfecta pf1225, a filamentous fungus that can degrade soybean saponin and generate soyasapogenol b. this enzyme was found to be a monomer with a molecular mass of about 77 kda and a glycoprotein. nucleotide sequence analysis of the corresponding gene (sdn1) indicated that this enzyme consisted of 612 amino acids and had a molecular mass of 65,724 da, in close agreement with that of the apoenzyme after the removal of ca ... | 2004 | 14766566 |
| cloning and characterization of saponin hydrolases from aspergillus oryzae and eupenicillium brefeldianum. | we purified saponin hydrolases from aspergillus oryzae pf1224 and eupenicillium brefeldianum pf1226. it was confirmed that the enzymes from a. oryzae pf1224 (sda1) and e. brefeldianum pf1226 (sde1) are glycoproteins with molecular masses of 82 and 90 kda respectively. the deduced amino acid sequences of each enzyme from the cloned genes (sda1 or sde1) showed approximately 50% homology with that of the saponin hydrolase sdn1 from neocosmospora vasinfecta var. vasinfecta pf1225 (ddbj accession no. ... | 2005 | 16306700 |
| uptake and metabolism of d-glucose by neocosmospora vasinfecta e. f. smith. | freshly harvested, nongrowing mycelium of neocosmospora vasinfecta e. f. smith rapidly absorbed exogenous glucose but converted a greater proportion to trehalose and glucan than to respiratory co(2). this effect was accentuated in mycelium preincubated for 3.5 hours in water before exposure to glucose. glucose was absorbed via two uptake systems, both apparently constitutive, with apparent km values for glucose of 0.02 mm (high affinity) and 2 mm (low affinity). the glucose derivative 3-o-methyl ... | 1976 | 16659646 |
| corneal ulcer due to neocosmospora vasinfecta in an immunocompetent patient. | we report a case of neocosmospora vasinfecta keratitis in a 55-year-old man. while the patient did not recall any specific trauma or eye injury, he might have sustained a trivial wound during the course of his duties as a farmer. direct examination of corneal scrapings revealed fungus filaments. as topical treatment with natamycin and econazole and subsequent systemic ketoconazole therapy failed, a full thickness therapeutic keratoplasty was performed. post-operative treatment with amphotericin ... | 2008 | 17885942 |
| viability of heterodera glycines exposed to fungal filtrates. | filtrates from nematode-parasitic fungi have been reported to be toxic to plant-parasitic nematodes. our objective was to determine the effects of fungal filtrates on second-stage juveniles and eggs of heterodera glycines. eleven fungal species that were isolated from cysts extracted from a soybean field in florida were tested on j2, and five species were tested on eggs in vitro. each fungal species was grown in czapek-dox broth and malt extract broth. no toxic activity was observed for fungi gr ... | 2000 | 19270965 |
| pathogenicity of fungi to eggs of heterodera glycines. | twenty-one isolates of 18 fungal species were tested on water agar for their pathogenicity to eggs of heterodera glycines. an egg-parasitic index (epi) for each of these fungi was recorded on a scale from 0 to 10, and hatch of nematode eggs was determined after exposure to the fungi on water agar for 3 weeks at 24 c. the epi for verticillium chlamydosporium was 7.6, and the fungus reduced hatch 74%. pyrenochaeta terrestris and two sterile fungi also showed a high epi and reduced hatch 42-73%. ar ... | 1996 | 19277130 |
| fungi associated with females and cysts of heterodera glycines in a florida soybean field. | fungal colonization was determined for females and cysts of heterodera glycines on soybean roots or in rhizosphere soil from a florida soybean field. a total of 1,620 females and cysts were examined in 1991, and 1,303 were examined in 1992. more than 35 species of fungi were isolated from females and cysts. the frequency of fungi colonizing white and yellow females was low, but a high frequency of fungi was encountered in brown cysts, which increased with time of exposure of the cysts to the soi ... | 1994 | 19279895 |
| isolation of fungi from heterodera glycines and in vitro bioassays for their antagonism to eggs. | twenty fungi were assayed in vitro for antagonism to eggs of heterodera glycines. eight of the fungi were isolated from cysts or eggs of h. glycines during the current study, one was isolated from panagrellus redivivus, and eleven were obtained from other researchers or collections. the bioassays were conducted on eggs from nematodes that had been grown monoxenically on excised root tips. phoma chrysanthemicola, one strain of verticillium chlamydosporium, and one strain of v. lecanii caused a de ... | 1990 | 19287754 |
| a disseminated infection with the antifungal-multiresistant teleomorphic fungus neocosmospora vasinfecta in a patient with acute b-lymphoblastic leukemia. | we report on a fatal invasive infection due to the ascomycetous fungus neocosmospora vasinfecta, in a 20-year-old european patient suffering from an acute lymphoblastic leukemia. the infection could not be controlled by a bitherapy combining liposomal amphotericin b and voriconazole. this is the second case of disseminated infection reported with this unusual fungus, which develops under its teleomorphic state, is fully resistant to all systemic antifungals, and which is known to live in tropica ... | 2013 | 24432214 |
| absolute stereochemistry of neovasinin, a phytotoxin produced by the fungus, neocosmospora vasinfecta. | | 1992 | 27286397 |