Publications

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genetic diversity of cryptosporidium spp. in captive reptiles.the genetic diversity of cryptosporidium in reptiles was analyzed by pcr-restriction fragment length polymorphism and sequence analysis of the small subunit rrna gene. a total of 123 samples were analyzed, of which 48 snake samples, 24 lizard samples, and 3 tortoise samples were positive for cryptosporidium: nine different types of cryptosporidium were found, including cryptosporidium serpentis, cryptosporidium desert monitor genotype, cryptosporidium muris, cryptosporidium parvum bovine and mou ...200414766569
molecular identification of a cryptosporidium saurophilum from corn snake (elaphe guttata guttata). 200717497169
molecular characterization of cryptosporidium and giardia from the tasmanian devil (sarcophilus harrisii).the tasmanian devil (sarcophilus harrisii) is a carnivorous marsupial found only in the wild in tasmania, australia. tasmanian devils are classified as endangered and are currently threatened by devil facial tumour disease, a lethal transmissible cancer that has decimated the wild population in tasmania. to prevent extinction of tasmanian devils, conservation management was implemented in 2003 under the save the tasmanian devil program. this study aimed to assess if conservation management was a ...201728423030
molecular identification of cryptosporidium isolates from exotic pet animals in japan.the cryptosporidium horse genotype, a zoonotic protozoan parasite first found in a prezewalski wild horse, has not been found in any other mammal but calves, horses, and humans. hedgehogs, popular exotic pet animals in japan, are a reservoir of two zoonotic cryptosporidum: c. parvum and c. erinacei (previously known as the hedgehog genotype). recently, after finding cryptosporidium infection in a four-toed hedgehog (atelerix albiventris), we identified the isolate genetically as the cryptosporid ...201525801359
the detection of cryptosporidium serpentis in snake fecal samples by real-time pcr.infection by cryptosporidium serpentis occurs in reptiles, particularly in snakes. this disease is characterized by chronic infection with the presence of hypertrophic gastritis. the objectives of this study were to use real-time polymerase chain reaction (pcr) targeting the heat shock protein 70 (hsp70) gene for the detection of c. serpentis in fecal samples from snakes and to determine the analytical and epidemiological specificity and sensitivity of this approach relative to the gold standard ...201424880646
Detection of Cryptosporidium species in feces or gastric contents from snakes and lizards as determined by polymerase chain reaction analysis and partial sequencing of the 18S ribosomal RNA gene.Cryptosporidiosis is a well-known gastrointestinal disease of snakes and lizards. In the current study, 672 samples (feces and/or gastric contents or regurgitated food items) of various snakes and lizards were examined for the presence of cryptosporidia by polymerase chain reaction (PCR) assay targeting a part of the 18S ribosomal RNA gene. A consecutive sequencing reaction was used to identify the cryptosporidian species present in PCR-positive samples. Cryptosporidium varanii (saurophilum) was ...201121908271
cloaca prolapse and cystitis in green iguana (iguana iguana) caused by a novel cryptosporidium species.cryptosporidium infection was associated with colitis and cystitis in 2 green iguanas (iguana iguana). the disease was characterized by a chronic clinical course of cloacal prolapses and cystitis. histological examination of the gut and urinary bladder showed numerous cryptosporidium developmental stages on the surface of the epithelium with mixed inflammatory response in the lamina propria. cryptosporidium oocysts were visualised in a cytological preparation of the faeces. based on the small su ...201021036480
cryptosporidium varanii infection in leopard geckos (eublepharis macularius) in argentina.cryptosporidiosis is observed in reptiles with high morbidity and considerable mortality. the objective of this study was to achieve the molecular identification of cryptosporidium spp. in pet leopard geckos (eublepharis macularius) from a breeder colony in buenos aires, argentina. oocysts comparable to those of cryptosporidium spp. were detected in three geckos with a history of diarrhea, anorexia and cachexia. molecular identification methods confirmed the presence of cryptosporidium varanii ( ...201627419102
cryptosporidium varanii takes precedence over c. saurophilum.we present biological as well as genetic analysis of cryptosporidium varanii at the 18s rrna and actin loci and show that it is genetically identical to c. saurophilum. as c. varanii was described prior to c. saurophilum, it takes precedence over c. saurophilum and therefore c. saurophilum should be considered a junior synonym of c. varanii.200817945215
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