| occurrence of cryptosporidium suis and cryptosporidium scrofarum on commercial swine farms in the czech republic and its associations with age and husbandry practices. | from 2009 to 2011, the occurrence of cryptosporidium spp. was investigated on 22 farms in the czech republic. a total of 1,620 individual faecal samples of pigs of all age categories (pre-weaned, starters, pre-growers, growers, and sows) were evaluated for presence of cryptosporidium spp. by standard microscopy and molecular tools. genotyping was done through pcr amplification and characterization of the ssu rrna (species-specific protocols) and gp60 loci. cryptosporidium spp. was found on 16 of ... | 2012 | 23271566 |
| cryptosporidium scrofarum n. sp. (apicomplexa: cryptosporidiidae) in domestic pigs (sus scrofa). | we describe the morphological, biological, and molecular characteristics of cryptosporidium pig genotype ii and propose the species name cryptosporidium scrofarum n. sp. to reflect its prevalence in adult pigs worldwide. oocysts of c. scrofarum are morphologically indistinguishable from c. parvum, measuring 4.81-5.96 μm (mean=5.16)×4.23-5.29 μm (mean=4.83) with a length to width ratio of 1.07±0.06 (n=400). oocysts of c. scrofarum obtained from a naturally infected pig were infectious for 8-week- ... | 2012 | 23021264 |
| presence of zoonotic cryptosporidium scrofarum, giardia duodenalis assemblage a and enterocytozoon bieneusi genotypes in captive eurasian wild boars (sus scrofa) in china: potential for zoonotic transmission. | cryptosporidium spp., giardia duodenalis and enterocytozoon bieneusi are the main causal pathogens of gastrointestinal disease. however, there are limited reports about the prevalence of these organisms in captive eurasian wild boars worldwide. therefore, we examined the occurrence and identified the species/assemblages/genotypes of these pathogens in captive eurasian wild boars, and estimated the zoonotic potential. | 2017 | 28061911 |
| cryptosporidium suis and cryptosporidium scrofarum in eurasian wild boars (sus scrofa) in central europe. | from 2011 to 2012, to identify cryptosporidium spp. occurrence in eurasian wild boars (sus scrofa) 29 randomly selected localities (both forest areas and enclosures) across the central european countries of austria, the czech republic, poland, and the slovak republic were investigated. cryptosporidium oocysts were microscopicaly detected in 11 out of 460 faecal samples examined using aniline-carbol-methyl violet staining. sixty-one cryptosporidium infections, including the 11 infections that wer ... | 2013 | 23916060 |
| occurrence, clinical involvement and zoonotic potential of endoparasites infecting swiss pigs. | in order to estimate the diversity, clinical involvement and zoonotic potential of parasites in pigs submitted for diagnosis to the pathopig project of the swiss federal food safety and veterinary office, faeces (n=125) from suckling piglets (n=39), weaners (n=60) and piglets beginning fattening (n=26) from 74 swiss farms were examined by 3 coproscopical methods (i.e. sedimentation/zinc chloride-flotation; safc and ziehl-neelsen staining). samples microscopically positive for cryptosporidium wer ... | 2016 | 27639842 |
| detection and identification of six cryptospordium species in livestock in slovakia by amplification of ssu and gp60 genes with the use of pcr analysis. | in this study we examined 200 faecal samples from pigs and calves with suspected cryptosporidiosis were examined by the pcr methods: nested pcr for amplification of ssu region; nested pcr for amplification of gp60 region; and with restriction analysis of dna (pcr-rflp). the sequencing identified the following species: cryptosporidium muris (2), cryptosporidium andersoni (1), cryptosporidium bovis (4), cryptosporidium suis (2), cryptosporidium scrofarum (10), mixed infection caused by c. scrofaru ... | 2016 | 27294628 |
| cryptosporidium species in sheep and goats from papua new guinea. | species of cryptosporidium are extensively recognised as pathogens of domesticated livestock and poultry, companion animals, wildlife, and are a threat to public health. little is known of the prevalence of cryptosporidium spp. in humans, domesticated animals or wildlife in papua new guinea (png). the aim of the present study was to screen sheep and goats for cryptosporidium using molecular tools. a total of 504 faecal samples were collected from sheep (n=276) and goats (n=228) in village, gover ... | 2014 | 24703974 |
| age-related detection and molecular characterization of cryptosporidium suis and cryptosporidium scrofarum in pre- and post-weaned piglets and adult pigs in japan. | we investigated the distribution of cryptosporidium in pigs in japan by immunofluorescence staining of fecal samples and characterization of isolates by multilocus sequencing. the 344 animals sampled on eight farms included pre-weaned piglets (<1 month old; n = 55), weaned piglets (1-2 months old; n = 65), finished pigs (2-4 months old, n = 105) and of 4-6 months old (n = 67), sows (n = 36), and boars (n = 16). average prevalence of cryptosporidium on farms was 32.6%, ranging from 4.9 to 58.1%, ... | 2014 | 24189974 |
| presence of cryptosporidium scrofarum, c. suis and c. parvum subtypes iiaa16g2r1 and iiaa13g1r1 in eurasian wild boars (sus scrofa). | the aim of the present study was to identify the species of cryptosporidium infecting eurasian wild boars (sus scrofa) in galicia (nw, spain). a sampling of 209 wild boars shot in different game preserves was carried out during the hunting season in 2009-2010. all samples were examined for cryptosporidium infection, using both immunological and molecular tools. cryptosporidium oocysts in faecal samples were identified using a direct immunofluorescence technique with monoclonal antibodies (dfa). ... | 2013 | 23643454 |
| cryptosporidium and giardia in danish organic pig farms: seasonal and age-related variation in prevalence, infection intensity and species/genotypes. | although pigs are commonly infected with cryptosporidium spp. and giardia duodenalis, including potentially zoonotic species or genotypes, little is known about age-related infection levels, seasonal differences and genetic variation in naturally infected pigs raised in organic management systems. therefore, the current study was conducted to assess seasonal and age-related variations in prevalence and infection intensity of cryptosporidium and giardia, evaluate zoonotic potential and uncover co ... | 2015 | 26483166 |
| cryptosporidium suis infection in post-weaned and adult pigs in shaanxi province, northwestern china. | cryptosporidium spp., ubiquitous enteric parasitic protozoa of vertebrates, recently emerged as an important cause of economic loss and zoonosis. the present study aimed to determine the distribution and species of cryptosporidium in post-weaned and adult pigs in shaanxi province, northwestern china. a total of 1,337 fresh fecal samples of post-weaned and adult pigs were collected by sterile disposable gloves from 8 areas of shaanxi province. the samples were examined by sheather's sugar flotati ... | 2015 | 25748718 |
| rodents as a reservoir of infection caused by multiple zoonotic species/genotypes of c. parvum, c. hominis, c. suis, c. scrofarum, and the first evidence of c. muskrat genotypes i and ii of rodents in europe. | cryptosporidium spp. is an important causative agent of intestinal parasitoses-induced diarrhoea in humans and animals worldwide. rodents (small mammals), the main reservoir of infections, are globally expanded and overpopulated, which increases the risk of transfer of human and zoonotic pathogens from the genus cryptosporidium. in this study, cryptosporidium was detected in wild immunocompetent asymptomatic small mammals. altogether 262 fecal samples were collected from five areas in eastern sl ... | 2017 | 28433573 |
| identification of pig-specific cryptosporidium species in mixed infections using illumina sequencing technology. | nowadays molecular methods are widely used in epidemiological studies of cryptosporidium infections in humans and animals. however to gain better understanding of parasite species or genotypes, especially when mixed infections are noticed, highly sensitive tools with adequate resolution power need to be employed. in this article, we report an application of the next generation sequencing method (ngs) for detection and characterisation of cryptosporidium species concurrently present in pig faeces ... | 2017 | 28939445 |
| age related susceptibility of pigs to cryptosporidium scrofarum infection. | piglets from 4 to 8 weeks of age originated from a cryptosporidium-free research breed were orally inoculated with 1 × 10(6) infectious oocysts of cryptosporidium scrofarum. the number of shed oocysts per gram of faeces served to describe the infection intensity and prepatent period. in addition, faecal samples collected daily and tissue samples of the small and large intestine collected at 30 days post-inoculation were examined for the c. scrofarum small subunit ribosomal rna gene using pcr. th ... | 2014 | 24630710 |