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survey on tick-borne pathogens in thoroughbred horses in the hidaka district, hokkaido, japan.a total of 87 thoroughbred horses and 10 ixodid ticks from a ranch in hidaka district, hokkaido were tested for tick-borne diseases. using the indirect fluorescent antibody (ifa) method, 3.4, 92.0 and 97.7% of the horses showed antibody titers of ≥ 80 against anaplasma phagocytophilum, rickettsia helvetica, and borrelia garinii, respectively. this is the first report of infection with the 3 pathogens in horses in japan. using pcr, dnas from the peripheral blood of all horses were found negative ...201322878540
detection of anaplasma bovis and anaplasma phagocytophilum dna from haemaphysalis megaspinosa in hokkaido, japan.dna from 101 ticks, including haemaphysalis megaspinosa, haemaphysalis douglasi, haemaphysalis flava, ixodes ovatus and ixodes persulcatus, collected by flagging in a pasture in hidaka district (hokkaido, japan) was examined for infection with anaplasma bovis and a. phagocytophilum by species-specific nested pcr. of the 48 h. megaspinosa nymphs, examined, 1 (2.1%) and 6 (12.5%) were positive for a. bovis and a. phagocytophilum, respectively. one of the 6 positive nymphs was dual positive for bot ...201019897306
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