| protein hpn: cloning and characterization of a histidine-rich metal-binding polypeptide in helicobacter pylori and helicobacter mustelae. | helicobacter pylori is a human gastrointestinal pathogen involved in gastritis, duodenal ulcers, and gastric neoplasia. this microorganism produces large amounts of a urease which, like all known ureases, has nickel in the active site. we have identified a protein in clinical isolates of h. pylori and an identical protein in the ferret pathogen helicobacter mustelae that strongly binds ni2+ and zn2+. this protein has been named hpn to emphasize its origins in h. pylori and its affinity for nicke ... | 1995 | 7790085 |
| identification and molecular characterization of a major ring-forming surface protein from the gastric pathogen helicobacter mustelae. | the spiral microaerophilic bacterium helicobacter mustelae is linked to gastritis and gastric ulcers in ferrets. electron microscopy of h. mustelae showed the presence of a laterally extensive array of 8.5-nm-diameter rings on the cell surface, which was shown to be composed of a 150kda protein. this protein was purified, and the sequence of 10 amino-terminal residues was determined. polyclonal antibody against the purified 150 kda protein labelled the ring structures on the homologous strain by ... | 1994 | 8170397 |
| an influenza a/h1n1/2009 hemagglutinin vaccine produced in escherichia coli. | the a/h1n1/2009 influenza pandemic made evident the need for faster and higher-yield methods for the production of influenza vaccines. platforms based on virus culture in mammalian or insect cells are currently under investigation. alternatively, expression of fragments of the hemagglutinin (ha) protein in prokaryotic systems can potentially be the most efficacious strategy for the manufacture of large quantities of influenza vaccine in a short period of time. despite experimental evidence on th ... | 2010 | 20661476 |
| characterization of hemolytic escherichia coli strains in ferrets: recognition of candidate virulence factor cnf1. | diseases associated with escherichia coli infection are the subject of renewed interest due to emerging conditions such as hemolytic uremia syndrome. a collection of 15 strains of beta-hemolytic e. coli was isolated from diarrheic feces and diseased tissues of ferrets. all 15 strains were positive in specific pcr assays for the presence of hlya, pap1, and cnf1. seven of the cnf1-positive isolates were tested and shown to have a cytopathic effect on hela cell monolayers. the pathogenesis of these ... | 2004 | 15583337 |
| an environmentally regulated pilus-like appendage involved in campylobacter pathogenesis. | examination of strains of campylobacter jejuni, campylobacter coli, and campylobacter fetus by electron microscopy revealed that they produced peritrichous pilus-like appendages when the bacteria were grown in the presence of bile salts. various bile-salt supplements were used and it was found that deoxycholate and chenodeoxycholic acid caused a significant enhancement of pilus production and resulted in a highly aggregative phenotype. morphologically, the pili were between 4 and 7 nm in width a ... | 1996 | 8793884 |
| transfer of antibody via mother's milk. | differing from humans, igg from breast milk in many animal species (rodents, bovines, cats, ferrets, etc.) are transported across the intestinal epithelium into the neonatal circulation. this transport is located at the duodenal and jejunal level where enterocytes express a surface membrane receptor able to bind fc of igg and to facilitate transcytosis of these immunoglobulins. fcgamma-r, which is very similar to the placenta receptor responsible for active transplacental transfer of igg in huma ... | 2003 | 12850343 |
| properly folded bacterially expressed h1n1 hemagglutinin globular head and ectodomain vaccines protect ferrets against h1n1 pandemic influenza virus. | in the face of impending influenza pandemic, a rapid vaccine production and mass vaccination is the most effective approach to prevent the large scale mortality and morbidity that was associated with the 1918 "spanish flu". the traditional process of influenza vaccine production in eggs is time consuming and may not meet the demands of rapid global vaccination required to curtail influenza pandemic. | 2010 | 20634959 |
| expression of biologically active recombinant ferret (mustela putorius furo) interleukin-8 from escherichia coli. | the authors expressed recombinant ferret interleukin-8 protein (rfril-8) in escherichia coli as a glutathione-s-transferase fusion protein. western blot analyses revealed that anti-ovine il-8 antibody reacted with rfril-8 at 10 kda. to confirm that the rfril-8 was biologically active, the authors examined chemotaxis and respiratory burst activity of ferret polymorphonuclear blood cells (pmns) exposed to rfril-8. the rfril-8 strongly induced chemotactic and respiratory burst activities in a stati ... | 2010 | 20678809 |
| mastitis caused by hemolytic escherichia coli in the ferret. | hemolytic escherichia coli was isolated from the mammary glands of 8 ferrets with gangrenous mastitis. clinical signs included firm swelling of one or more mammary glands and discoloration of the overlying skin. peracute disease and acute septicemia were observed, and in some cases the animals rapidly became moribund. antibiotic therapy alone did not alter the course of the disease. wide surgical resection of the involved glands in combination with systemic antibiotic therapy (ampicillin 10 mg/k ... | 1983 | 6358164 |
| the biochemical characterization of ferret carotene-9',10'-monooxygenase catalyzing cleavage of carotenoids in vitro and in vivo. | previous studies have shown that beta-carotene 15,15'-monooxygenase catalyzes the cleavage of beta-carotene at the central carbon 15,15'-double bond but cleaves lycopene with much lower activity. however, expressing the mouse carotene 9',10'-monooxygenase (cmo2) in beta-carotene/lycopene-synthesizing and -accumulating escherichia coli strains leads to both a color shift and formation of apo-10'-carotenoids, suggesting the oxidative cleavage of both carotenoids at their 9',10'-double bond. here w ... | 2006 | 16672231 |
| ferrets as a model system for renal disease secondary to intestinal infection with escherichia coli o157:h7 and other shiga toxin-producing e. coli. | ferrets were evaluated as a possible small animal model for the development of colitis and/or signs of the hemolytic uremic syndrome after oral infection with escherichia coli o157:h7 or other shiga toxin--producing e. coli (stec). ferrets treated with streptomycin (stm) had higher counts of e. coli o157:h7 strain 86-24 stm-resistant (stm(r)) or o91:h21 strain b2f1 stm(r) in their stools than non--stm-treated animals. none of the animals displayed evidence of colitis, but stm-treated animals fed ... | 2002 | 11865409 |
| pre-clinical and clinical investigation of the safety of a novel adjuvant for intranasal immunization. | nasalflu berna is a trivalent influenza virus vaccine for active immunization against influenza by the nasal route. it consists of influenza virosomes which are formulated from inactivated influenza strains and heat-labile toxin from aseptic escherichia coli bacteria strain, as an adjuvant (hlt). the results of preclinical studies in ferrets, baboons, minipigs, mice and rabbits are presented here, and issues concerning route of administration, mechanism of action (preventing the disease and halt ... | 2001 | 11587809 |
| cloning and quantification of ferret serum amyloid a. | serum amyloid a (saa) is used as a biomarker for infections and inflammation in humans and veterinary medicine. we cloned ferret cdna encoding saa from the liver of a ferret via reverse transcription pcr (rt-pcr). the sequence of the cdna clone revealed that ferret saa has an open reading frame of 387 bp that encodes 129 amino acids. the deduced amino acid sequence of ferret saa has 96.1, 89.9, 86.0, 83.8, 83.0, 73.8 and 65.3% similarity to the mink, dog, cat, cattle, horse, human and mouse saa ... | 2013 | 22972465 |
| enterotoxigenic escherichia coli infection in captive black-footed ferrets. | enterotoxigenic escherichia coli with genes for heat stabile toxins sta and stb was isolated from the gastrointestinal tract and multiple visceral organs of three adult and three juvenile black-footed ferrets (mustela nigripes) that died in a captive breeding colony between 24 may 1998 and 2 july 1998. similar isolates were obtained from rectal swabs of one adult and one juvenile that were clinically ill. all were fed a diet composed of mink chow, raw rabbit meat, beef liver powder, blood meal a ... | 2001 | 11504237 |
| a phase-variable capsule is involved in virulence of campylobacter jejuni 81-176. | campylobacter jejuni strain 81-176 (hs36, 23) synthesizes two distinct glycan structures, as visualized by immunoblotting of proteinase k-digested whole-cell preparations. a site-specific insertional mutant in the kpsm gene results in loss of expression of a high-molecular-weight (hmw) glycan (apparent mr 26 kda to > 85 kda) and increased resolution of a second ladder-like glycan (apparent mr 26-50 kda). the kpsm mutant of 81-176 is no longer typeable in either hs23 or hs36 antisera, indicating ... | 2001 | 11359581 |
| haemolysin production by strains of verocytotoxin-producing escherichia coli. | twenty-one strains of verocytotoxin-producing escherichia coli (vtec) that hybridized with dna probe cvd419 were examined for the ability to produce haemolysin. with solid media, all strains produced most haemolysin when grown in blood agar tubes and least when grown on blood agar plates incubated in air. haemolysin production was increased considerably by incubating blood agar plates in an atmosphere comprising 8% carbon dioxide, 40% hydrogen and 52% nitrogen at 37 degrees c for 16 h, followed ... | 1998 | 9467902 |
| disseminated cutaneous squamous cell carcinoma in a ferret. | a 3-year-old male ferret was admitted with ulcerated nodules and fistulous draining tracts on the pads and digits of the right front and left hind feet. proteus sp and escherichia coli were isolated from the exudate. histologic examinations revealed that all skin lesions were associated with an underlying squamous cell carcinoma. surgical removal was attempted, followed by chemotherapy. regression of remaining lesions was noted before discharge; however, the ferret died 5 days later and was not ... | 1985 | 3988603 |
| enhanced neutralizing antibody titers and th1 polarization from a novel escherichia coli derived pandemic influenza vaccine. | influenza pandemics can spread quickly and cost millions of lives; the 2009 h1n1 pandemic highlighted the shortfall in the current vaccine strategy and the need for an improved global response in terms of shortening the time required to manufacture the vaccine and increasing production capacity. here we describe the pre-clinical assessment of a novel 2009 h1n1 pandemic influenza vaccine based on the e. coli-produced ha globular head domain covalently linked to virus-like particles derived from t ... | 2013 | 24204639 |
| h5 n-terminal β sheet promotes oligomerization of h7-ha1 that induces better antibody affinity maturation and enhanced protection against h7n7 and h7n9 viruses compared to inactivated influenza vaccine. | initiation of mass vaccination is critical in response to influenza pandemic. there is an urgent need of a simple, rapid method for production of influenza vaccine that is more effective than current traditional influenza vaccines. recent h7n9 transmissions to humans in china with high morbidity/mortality initiated extensive vaccine evaluation. we produced the ha1 domains (amino acids 1-320) from h7n9 and h7n7 strains in e. coli. both were found to contain primarily monomers/trimers with low oli ... | 2014 | 25284811 |
| nitrate biosynthesis in rats, ferrets and humans. precursor studies with l-arginine. | l-arginine, the primary nitrogen source for nitric oxide synthesized by many cell types in culture and for biosynthesized nitrate in humans, is also a nitrogen source for biosynthesized nitrate in rats and ferrets. after administration of [15n2]l-arginine to rats and ferrets, [15n]no3- was detected in urine. escherichia coli lipopolysaccharide induced more than a 10-fold increase in urinary nitrate in rats and a parallel increase in incorporation of 15n from [15n2]l-arginine into no3-. bradykini ... | 1990 | 2335012 |
| nontoxic outer membrane vesicles efficiently increase the efficacy of an influenza vaccine in mice and ferrets. | in this study, we developed a further-modified outer membrane vesicle (fmomv) from the δmsbb/δpagp mutant of escherichia coli transformed with the plasmid, plpxf, in order to use it as an adjuvant for pandemic h1n1 (ph1n1) influenza vaccine. we evaluated the efficacy of the ph1n1 influenza vaccine containing the fmomv in animal models as compared to the commercial adjuvants, alum or addavax(tm). the fmomv-adjuvanted ph1n1 influenza vaccine induced a significant increase in the humoral immunity; ... | 2017 | 28576571 |
| immunogenicity and safety of h1n1 influenza hemagglutinin protein expressed in a baculovirus system. | although most influenza vaccines are produced in eggs, new types of vaccines must be developed. in this study, the immunogenicity and safety of a baculovirus-expressed hemagglutinin (ha) of h1n1 influenza virus (korea/01/2009; designated "ha-bac-k") was compared with those of a commercially available baculovirus-expressed ha (designated "ha-bac-c") and an escherichia coli-expressed ha (designated "ha-e. coli-k"). ha-bac-k succeeded in inducing hemagglutination inhibition and neutralization antib ... | 2013 | 23822120 |
| vaccination with different m2e epitope densities confers partial protection against h5n1 influenza a virus challenge in chickens. | objective: currently, research is focused on universal influenza vaccines based on various ectodomains of the influenza matrix protein 2 (m2e). such vaccines are tested mostly using mouse-adapted influenza viruses and in mouse or ferret models. the aim of this study was to investigate in a chicken model the protective efficacy of vaccines based on avian-type m2e at different epitope densities. methods: on the basis of the optimized avian-type m2e gene, recombinant plasmids that contained tandem ... | 2011 | 21228535 |
| bacterial ha1 vaccine against pandemic h5n1 influenza virus: evidence of oligomerization, hemagglutination, and cross-protective immunity in ferrets. | the impending influenza virus pandemic requires global vaccination to prevent large-scale mortality and morbidity, but traditional influenza virus vaccine production is too slow for rapid responses. we have developed bacterial systems for expression and purification of properly folded functional hemagglutinin as a rapid response to emerging pandemic strains. a recombinant h5n1 (a/vietnam/1203/2004) hemagglutinin globular domain (ha1) was produced in escherichia coli under controlled redox refold ... | 2010 | 21084473 |
| the receptor-binding domain of influenza virus hemagglutinin produced in escherichia coli folds into its native, immunogenic structure. | the hemagglutinin (ha) surface glycoprotein promotes influenza virus entry and is the key protective antigen in natural immunity and vaccines. the ha protein is a trimeric envelope glycoprotein consisting of a globular receptor-binding domain (ha-rbd) that is inserted into a membrane fusion-mediating stalk domain. similar to other class i viral fusion proteins, the fusogenic stalk domain spontaneously refolds into its postfusion conformation when expressed in isolation, consistent with this doma ... | 2010 | 21068239 |
| safe recombinant outer membrane vesicles that display m2e elicit heterologous influenza protection. | recombinant, escherichia coli-derived outer membrane vesicles (romvs), which display heterologous protein subunits, have potential as a vaccine adjuvant platform. one drawback to romvs is their lipopolysaccharide (lps) content, limiting their translatability to the clinic due to potential adverse effects. here, we explore a unique romv construct with structurally remodeled lipids containing only the lipid iva portion of lps, which does not stimulate human tlr4. the romvs are derived from a genet ... | 2017 | 28215994 |