| long-term function of euro-collins--preserved human cardiac allografts. | | 1992 | 1412952 |
| [status of treatment with free radical scavengers following kidney and pancreas transplantation]. | the frequency of acute renal failure (arf) after preservation and kidney transplantation is rather high. the etiology of an arf is an interaction of multiple mechanisms as donor conditions, explantation procedure, duration of ischemia and reperfusion injury. different experiments demonstrated, that the damage produced by reperfusion can be prevented by the scavenger of free radicals, the enzyme superoxide dismutase (sod). | 1992 | 1441789 |
| a comparison of human islet purification with discontinuous density gradients of ficoll prepared with euro-collins or medium 199 solution. | | 1992 | 1465957 |
| long-term preservation of canine pancreas by a new simple cold storage method using perfluorochemical--the two-layer cold storage method (euro-collins' solution/perfluorochemical). | long term preservation of canine pancreas by a new simple cold storage method using perfluorochemical (pfc), two-layer (euro-collins' solution (ec)/pfc) cold storage method, was tested in the canine model of segmental pancreas autotransplantation. the functional recovery of the graft preserved by this method (group 1) was determined by daily fasting blood glucose concentration and intravenous glucose tolerance test at two weeks after autotransplantation and compared with simple cold storage with ... | 1992 | 1518272 |
| twenty-four-hour hypothermic preservation of rat liver with euro-collins and uw solutions. a comparative evaluation by 31p nmr spectroscopy, biochemical assays, and light microscopy. | a comparative study of 24 hr preservation at 4 degrees c of excised rat livers with euro-collins and hydroxyethyl starch-free university of wisconsin (uwm) solutions has been conducted based on the assessment of (1) the cellular energy status determined by 31p nmr spectroscopy and (2) cellular injury estimated from the loss of purine compounds (inosine, hypoxanthine, xanthine, and uric acid) during cold ischemia and reperfusion measured by hplc, the leakage of intracellular enzymes, and the modi ... | 1992 | 1412750 |
| the effect of prostaglandin e1 on heart preservation. | the purpose of this study was to investigate the effect of prostaglandin e1 on rabbit heart preservation. the hearts were arrested with cardioplegia (cp) solution, followed by intracoronary infusion of euro-collins (e-c) solution. they were immersed in e-c solution at 4 degrees c for 9 hr, flushed with terminal warm cp solution, and reperfused with oxygenated tyrodes' solution at 80 mmhg for 90 min. eighteen rabbits (kabushikigaishya biotec japanese white) were divided into 3 groups. group i ser ... | 1992 | 1412720 |
| oxygen free radical scavengers and reperfusion injury in dog lung preserved in cold ischemia. | oxygen-derived free radicals are now considered important contributors to tissue injury associated with ischemia and reperfusion. the purpose of this study was to determine the influence of oxygen free radical scavengers on reperfusion injury. the left lower lobes of 15 canine lungs were isolated, preserved, and then reperfused for 120 minutes. three groups of lobes were studied: group 1 (n = 5), without ischemia, group 2 (n = 5) four hours of cold ischemia in euro-collins solution, group 3 (n = ... | 1992 | 1412381 |
| an experimental comparison between isotonic saline solution, euro-collins, and a flush solution with mannitol in the prevention of renal damage due to warm ischemia. | | 1992 | 1539341 |
| protective effects of coenzyme q10 on ischemia-induced reperfusion injury in ischemic limb models. | we examined simple cold preservation of rat limbs in euro-collins' solution to elucidate the protective effect of coenzyme q10 (coq10) on the ischemia-induced reperfusion injury in an ischemic extremity replant model. a total of 126 lewis rat limb replants were performed. limbs were amputated from donor rats and preserved at 4 degrees c in euro-collins' solution and were orthotopically grafted to isogeneic rats by microsurgical technique. in the experimental groups (n = 42), coenzyme q10 (10 mg/ ... | 1992 | 1410041 |
| reliable thirty-hour lung preservation by donor lung hyperinflation. | we examined the hypothesis that the degree of inflation of the lungs at the time of harvest may have an important role in postpreservation function. lungs of donor dogs randomly assigned to groups 1 (n = 5) and 2 (n = 5) were ventilated with large tidal volumes (tidal volume, 25 ml/kg; positive end-expiratory pressure, 5 cm h2o; respiratory rate, 12 breaths/min, inspired oxygen fraction 1.0) and were inflated to 30 cm h2o for 15 seconds before pulmonary artery flush and again immediately before ... | 1992 | 1405666 |
| phosphorus 31-nuclear magnetic resonance spectroscopy of rat liver during simple storage or continuous hypothermic perfusion. | the adenosine triphosphate (atp) content and intracellular ph (phi) of isolated rat liver before, during, and after cold preservation in either university of wisconsin lactobionate solution (uw) (n = 10) or euro-collins solution (ec), (n = 8) were monitored with phosphorus 31 nuclear magnetic resonance. the 31p nuclear magnetic resonance spectra were obtained on a 4.7 t system operating at 81 mhz. fructose metabolism, liver enzyme release, oxygen consumption, and rat survival after liver transpl ... | 1992 | 1402332 |
| transient activation of hepatic glycogenolysis by thrombin in perfused rat livers. | thrombin, a peptide with native protease activity, caused a rapid (less than 1 min) increase in glycogenolysis of about 30%, assessed from rates of production of glucose+lactate+pyruvate, and in oxygen uptake in perfused rat liver. these increases were followed by a rapid return to basal values within 5 min. the effect of thrombin on glycogenolysis was dose-dependent and was maximal at perfusate concentrations around 1 u/ml. interestingly, the effect of thrombin on glycogenolysis could be elicit ... | 1992 | 1396679 |
| protective effects of diltiazem and the prostazycline analogue iloprost in human renal transplantation. | to test the hypothesis that calcium antagonists decrease the incidence and severity of delayed graft function, we conducted three separate, prospective, randomized trials. in these trials, we investigated the effects of diltiazem and those of the prostacycline analogue iloprost. in the first study, 22 control patients and 20 diltiazem patients received grafts perfused with either vehicle or diltiazem 20 mg/l in the euro-collins solution. subsequently, the diltiazem subjects were given the drug a ... | 1992 | 1380721 |
| equivalent eighteen-hour lung preservation with low-potassium dextran or euro-collins solution after prostaglandin e1 infusion. | improved techniques of pulmonary preservation would help alleviate the critical shortage of donor organs in lung transplantation and would improve early graft function. a previous study demonstrated that cold pulmonary artery flush with low-potassium dextran solution was superior to euro-collins solution in preservation of canine lung allografts stored for 12 hours when no pulmonary vasodilator was used before donor lung flush. the present study was designed to determine whether donor pretreatme ... | 1992 | 1377316 |
| protective effects of calcium antagonists in human renal transplantation. | to test the hypothesis that calcium antagonists decrease the incidence and severity of delayed graft function, we conducted three separate, prospective, randomized trials. in these trials, we investigated the effects of diltiazem and those of the prostacyclin analogue iloprost. in the first study, 22 control patients and 20 diltiazem patients received grafts perfused with either vehicle or diltiazem 20 mg/liter in the euro-collins solution. subsequently, the diltiazem subjects were given the dru ... | 1992 | 1377295 |
| unilateral lung transplantation: ultrastructural studies of ischemia-reperfusion injury and repair in the canine pulmonary vasculature. | the left lung was transplanted in 12 adult beagles; the donor lung had been preserved by flush perfusion of the pulmonary artery with 60 ml/kg of euro-collins solution at 4 degrees c then stored in euro-collins solution at 4 degrees c for 6 hours. biopsy specimens were taken from control and donor left lungs before and immediately after perfusion, after 6 hours storage, and after transplantation at 4 and 24 hours (total = 46). tissue from seven animals was injected with the tracer ruthenium red. ... | 1992 | 1371699 |
| the role of dextran 40 and potassium in extended hypothermic lung preservation for transplantation. | we have previously demonstrated that a low-potassium dextran solution provides superior and more reliable preservation of lungs for 12 hours than that provided by the commonly used euro-collins solution. this study was designed to examine the individual contributions of dextran 40 and a low (extracellular) potassium concentration to lung preservation. in a randomized, blinded study using an in vivo canine single-lung transplant model, lungs preserved with low-potassium dextran solution (k+, 4 mm ... | 1992 | 1370970 |
| immunoscintigraphic localization of renal tumours in an extracorporeal perfusion model with a monoclonal antibody against gamma-glutamyltransferase. | monoclonal antibody 138h11 against human gamma-glutamyltransferase has been shown to react immunohistochemically with 98% of all tested clear-cell type and chromophilic renal cell carcinomas, but not with renal chromophobic carcinomas, duct-bellini carcinomas or oncocytomas. in normal kidney the target epitopes of mab 138h11 are located in the luminal brush-border membrane of proximal tubule cells, whereas in renal carcinomas the epitopes are found surrounding the whole tumour cells. these resul ... | 1992 | 1355011 |
| evidence that free radicals are involved in graft failure following orthotopic liver transplantation in the rat--an electron paramagnetic resonance spin trapping study. | the purpose of these studies was to determine whether free radicals were formed as a consequence of reperfusion during orthotopic liver transplantation and whether their formation was related to graft failure. grafts were stored for 18 hr in euro-collins solution or for 48 hr in university of wisconsin solution (nonsurvival conditions) and reperfused with blood containing the spin trap alpha-phenyl n-tert-butylnitrone (pbn). venous blood samples (4-5 ml) were collected, and serum was extracted w ... | 1992 | 1323148 |
| hepatic energy metabolism during hypothermic storage and reperfusion using different protecting solutions. | effects of 5 cold storage solution on hepatic high energy phosphate metabolism and metabolic function were examined using the isolated perfused rat liver. university of wisconsin (uw), euro-collins (ec), and 2 cardioplegic solutions, bretschneider's htk and st. thomas hospital solution, were studied for their protective capacity. krebs-henseleit bicarbonate buffer (khb) was used to point out the effect of simple hypothermia. liver atp, total adenine nucleotides and energy charge losses were sign ... | 1992 | 1292938 |
| effects of dehydration on body-water distribution in desert kangaroos. | the effect of dehydration on the distribution of water in the bodies of two species of desert kangaroos, the red kangaroo megaleia rufa and the euro macropus robustus, has been examined. the volumes of various body-fluid compartments were determined in normally hydrated animals and then after the kangaroos had been dehydrated until body weight declined to 80% of the initial weight. the fluid compartments examined were total body water, plasma volume, intracellular volume (cellular and gut water) ... | 1975 | 1147052 |
| structures of biogenic origin from early precambrian rocks of euro-asia. | spheroidal microfossils mainly 20 to 100 mug in diameter and exhibiting granular surface textures have been recovered from early precambrian rocks by applying a new method of water separation in combination with thin chemical preparation. in contrast to the acritarcha, these microfossils are characterized by a relatively low specific weight (close to one) and considerable fragility due to impregnation by mineral matter. they occur in archean sediments of hindustan, in rocks of the baltic and ald ... | 1975 | 807898 |
| [goethe's morphology definition and the problem of relationship of the cro-magnons to the primitive germans (carl gegenbaur)]. | the problem of the teutons' origin has been considered to be open up to this day though some scientists have worked on this field since 1912. anthropologists and linguists as braun, feist, guntert, koppelmann, paudler and v.d. velden produced many evidences in favour of the hypothesis the teutons to be a mixture of the old european cro-magnons speaking an archaic ural-altaic idiom with the indo-europeans immigrating into europe from asia. the kelts may have a similar origin but they might includ ... | 1978 | 357241 |
| successful 96-hr cold-storage preservation of canine pancreas with uw solution containing the thromboxane a2 synthesis inhibitor oky046. | prostanoids, such as prostacyclin (pgi) and thromboxane a2 (txa), have been recently suggested to play an important role in preservation-induced injury of pancreas grafts. we have previously shown that the txa synthesis inhibitor oky046 prevents a decrease of both the pgi/txa ratio and blood flow in pancreas grafts after 24-hr preservation with euro-collins solution. in our present study, we analyzed whether oky046 added to university of wisconsin (uw) solution could extend successful cold-stora ... | 1992 | 1528034 |
| low-temperature fluorometric technique for evaluating the viability of rat liver grafts after simple cold storage. | time-dependent changes in the viability of rat liver graft during cold preservation with euro-collins solution were evaluated with nadh fluorometry. correlation between the fluorometric analysis, 1-week survival rate after liver transplantation, and mitochondrial atp synthesis activity in the early phase after transplantation was studied. fluorometric study: rat livers were preserved at 0 degree-4 degrees c for 0-48 h in euro-collins solution and then reperfused for 15 min with oxygenated krebs- ... | 1992 | 1514964 |
| microvascular changes of the liver preserved in uw solution. pathological and immunohistochemical examination. | rat livers preserved in university of wisconsin (uw) solution for 24 h were compared with those preserved in euro-collins (ec) solution before and after liver transplantation using an immunohistochemical method. tissue atp and total tissue adenine nucleotide (tan) were measured using hplc. the levels of tan in the uw group or the ec group were significantly low compared with the control group (no preservation) after 24-h storage. in the ec group, the levels of tissue adenine nucleotides (tan) de ... | 1992 | 1499316 |
| improvement of currently used methods for lung preservation with prostacyclin and university of wisconsin solution. | after 6 hours of cold ischemia the quality of lung preservation was assessed in a canine heart-lung transplant model comparing three clinically used methods for lung preservation: donor core cooling by means of extracorporeal circulation (group i); pulmonary artery flush perfusion with either euro-collins solution after prostacyclin application (group ii); or university of wisconsin solution after donor pretreatment with prostacyclin (group iii). in all cases st. thomas hospital solution was use ... | 1992 | 1498128 |
| reduction of cya nephrotoxicity by nifedipine during and after experimental in situ renal preservation. | in order to evaluate the role of nifedipine in the nephrotoxic effect induced by both ischemia and cya, 18 healthy mongrel dogs were used. the kidneys were exposed and subjected to 1 h of ischemia by clamping both renal vessels. to the renal artery of the first group of kidneys (n = 9), 300 ml of cold euro-collins solution, in which nifedipine (bay a 1040-10 mg) was diluted, was infused for 15 min (nifedipine group), while 300 ml of cold euro-collins solution plus 10 mg of placebo (bay a 1040-pl ... | 1992 | 1461999 |
| effects of allopurinol pretreatment with pulmonary flush on lung preservation. | this study was designed to test whether use of allopurinol could improve lung preservation after 6 hours of cold storage. thirty-two rabbits were divided into four groups (n = 8 each group): (1) the control group received no flush or storage, (2) the ec group received euro-collins (ec) solution for both flush and storage, (3) the allo-f group received euro-collins solution with allopurinol (1 mmol/l) for both flush and storage, and (4) the allo-r group received euro-collins solution to which all ... | 1992 | 1457426 |
| vascular and interstitial effect of university of wisconsin solution on canine lung. | belzer's university of wisconsin cold storage solution (uwcss) has proved useful in extending the shelf life of organs in extrathoracic transplantation and more recently has also been shown to be useful in heart transplantation. i investigated the effect of 4 degrees c uwcss on the vascular and interstitial properties of the lung to see whether it affected the pulmonary microcirculation or caused pulmonary edema. infusion of uwcss was associated with a slight decrease in oxygen tension, but the ... | 1992 | 1449305 |
| platelet activating factor antagonist enhances lung preservation. | platelet activating factor (paf) is a potent phospholipid mediator of the immune and inflammatory responses, which causes physiologic effects similar to post-transplant pulmonary dysfunction. this study investigates the hypothesis that the use of a specific paf antagonist (pafa), bn 52021, in canine lung transplantation improves lung preservation. twelve pairs of canines underwent left lung allotransplantation after pulmonary artery flushing with modified euro-collins (ec) solution (40 ml/kg). t ... | 1992 | 1528038 |
| university of wisconsin solution for pulmonary preservation in a rat transplant model. | university of wisconsin and modified euro-collins solutions for pulmonary preservation were compared in a rat orthotopic left lung isotransplant model. heart-lung blocks of donor rats were flushed with and preserved in one of the preservation solutions at 0 degrees c. after 6 or 12 hours of cold ischemia, the left lungs were transplanted into recipient rats and reperfused for 1 hour. pulmonary function was assessed by measuring oxygen and carbon dioxide tensions in arterial blood after removal o ... | 1992 | 1531007 |
| the role of enhanced mitochondrial phosphorylation in rat liver transplantation. | the effects of organ preservation on mitochondrial oxidative phosphorylation activity, adenylate hepatic energy charge, cytochrome content, and redox state of nad+/nadh couple in rat liver transplantation were compared between a nonpreservation group and a preservation group with grafts preserved for 12 hr in euro-collin's solution. at 3 hr after transplantation, the energy charge in the preservation group decreased to 0.60 +/- 0.02 from the control value of 0.86 +/- 0.01, accompanied by a reduc ... | 1992 | 1548869 |
| a prolongation of hepatic vascular exclusion by in situ hypothermic perfusion in dogs. | in situ hypothermic hepatic perfusion was performed in dogs to explore whether the time limit of hepatic vascular exclusion could be prolonged. during hepatic vascular exclusion, hepatic hypothermic perfusion was performed via portal vein using various perfusates under active bypass from the portal vein and infrahepatic inferior vena cava area to the superior vena cava system. dogs receiving hepatic hypothermic perfusion for 1 hour died when given ringer's solution but survived more than 7 days ... | 1992 | 1558496 |
| protection by coenzyme q10 of canine myocardial reperfusion injury after preservation. | this study was undertaken to assess whether pretreatment of the donor heart with coenzyme q10 could improve postischemic myocardial recovery. coenzyme q10 or its solvent was administered intravenously to donor dogs 1 hour before isolation of the heart. each heart was stored in euro-collins solution at 4 degrees c for 6 or 24 hours and then was reperfused via the aorta with arterial blood of a supporting dog for 3 hours at 37 degrees c in a working mode. during preservation, adenosine triphosphat ... | 1992 | 1569776 |
| improvement in experimental cardiac preservation based on metabolic considerations. | viability of rat hearts preserved in the standard euro-collins and a modified preservation medium utilizing the euro-collins medium as the base were assessed. the additions for the modified medium were chosen in order to attenuate the various forms of damage that may occur to an organ during cold ischemia. the viability of the hearts was assessed biochemically via 31p nuclear magnetic resonance spectroscopy and functionally during reperfusion in an isolated langendorff preparation. it was noted ... | 1991 | 1750061 |
| [protection of the tunica intima during preservation of venous bypass grafts from harvesting to implantation]. | the patency-rate of venous bypasses depends--beside other important factors--upon the integrity of the transplanted vessel, especially on the condition of the tunica intima. we investigated two different methods of venous storage and their on the intima. we examined 24 segments of healthy human saphenous vein and 65 segments of the inferior vena cava in the rat which had been harvested using a minimal touch technique. the veins were stored in the patients own arterial heparinized blood vs. euro ... | 1992 | 1582861 |
| morphological investigation of the porcine liver directly following preservation with euro-collins, university of wisconsin and bretschneider's htk solution. | in order to study perfusion effects of different liver preservation methods on liver structure, porcine livers were perfused with either bretschneider's htk (histidine tryptophane ketoglutarat) solution, euro-collins (ec) solution or university of wisconsin solution (uw) according to the respective recommended protocols. subsequently, together with a group of unprotected livers, all organs were examined by light and electron microscopy including computer assisted morphometry. the width of the sp ... | 1992 | 1583986 |
| the mechanism of action of the two-layer (euro-collins' solution/perfluorochemical) cold-storage method in canine pancreas preservation--the effect of 2,4 dinitrophenol on graft viability and adenosine triphosphate tissue concentration. | tissue concentrations of adenosine triphosphate have been previously associated with successful pancreas preservation using the two-layer cold storage method in a canine autotransplantation model. to clarify the role of atp vs. oxygenation per se, we used 2,4 dinitrophenol, an uncoupler of mitochondrial oxidative phosphorylation. dnp caused no toxicity in pancreas grafts preserved for 24 hr in euro-collins' solution or 48 hr in university of wisconsin solution. tissue concentration of atp and vi ... | 1992 | 1585492 |
| reliable eighteen-hour lung preservation at 4 degrees and 10 degrees c by pulmonary artery flush after high-dose prostaglandin e1 administration. | pulmonary preservation is improved by hypothermia, but the optimal preservation temperature is not known. the effects of two different preservation temperatures, 4 degrees and 10 degrees c, on lung function were studied in a canine left lung allograft survival model allowing selective perfusion of either lung. after donor treatment with high-dose prostaglandin e1, (25 micrograms/kg), lungs were flushed with modified euro-collins solution (50 ml/kg) and stored in euro-collins solution for 18 hour ... | 1992 | 1597977 |
| evaluation of uw solution for preservation of small intestinal transplants in the rat. | simple cold preservation was evaluated in the rat model of small intestinal transplantation. lewis rats received a syngeneic heterotopic graft of the jejunum either immediately (si) or after preservation for 24 hr in euro-collins (spe24), for 48 hr in ec (spe48), for 24 hr in university wisconsin solution (spw24), or for 48 hr in uw (spw48). the survival rates of si, spe24, spe48, spw24, and spw48 were 100%, 78%, 0%, 100%, and 33%, respectively. physiologic and pharmacologic properties of the gr ... | 1992 | 1604473 |
| stimulation of monooxygenation and conjugation after liver transplantation in the rat: involvement of kupffer cells. | the success rate of liver transplantation has improved markedly during the last few years and, although this patient population receives multiple drug therapies, the effect of liver transplantation on drug metabolism has been studied very little. therefore, the purpose of this study was to assess the metabolism of model drug substrates after liver transplantation in the rat. rat livers were stored for 4 hr in cold euro-collins solution, transplanted orthotopically, and then perfused 2 hr later w ... | 1992 | 1614413 |
| ischemic-type biliary complications after orthotopic liver transplantation. | nonanastomotic biliary strictures that involve only the biliary tree of the graft occur after orthotopic liver transplantation in patients with hepatic artery thrombosis, chronic ductopenic rejection and abo blood group incompatibility. this complication may also occur in the absence of these known risk factors. the major focus of our study was to evaluate the risk factors for nonanastomotic biliary stricturing of unknown cause after orthotopic liver transplantation. results demonstrate that the ... | 1992 | 1618482 |
| pulmonary arterial impedance after single lung transplantation. | single lung transplantation (slt) is emerging as definitive therapy for end-stage pulmonary disease of varying etiology, yet a complete description of the hemodynamic properties of the transplanted lung has not been reported. in this study, fourier analysis was used to calculate the pulmonary arterial (pa) impedance spectrum before and immediately after slt to define precisely the pulmonary pressure-flow relationship. median sternotomies were performed in 18 dogs (donors): an ultrasonic flow pro ... | 1992 | 1619914 |
| the use of the university of wisconsin (uw) and euro-collins (ec) solutions either alone or in a combined method. | from june 1988 to october 1990, a total of 100 orthotopic liver transplantations (olts) in 91 patients were performed at the hospital clínic of barcelona. euro-collins (ec) solution was used as the flush and storage solution in 29 livers, and the university of wisconsin (uw) solution was used in 24. a combined method, consisting of flushing and harvesting the liver with uw solution through the portal vein and with ec solution through the aorta, was used in the remaining 47 livers. livers harvest ... | 1992 | 1627243 |
| [international control of the quality of microscopic-numerical analysis of the levels of man-made mineral fibers (mmmf) in the air of the work area]. | in the years 1986-1990 the institute of occupational medicine in lodz (department of aerosols) participated in seven exchanges of preparations within the who/euro mmmf reference scheme programme. the number of persons analysing the preparations increased from three, during the first exchange, to seven in the sixth. markings in the 1st and 3rd exchange constituted two thirds of the reference markings. in the 2nd, 5th and 7th exchange the markings were similar, and in the 6th exchange they were lo ... | 1992 | 1635445 |
| the use of a prostacyclin analog, iloprost, as an adjunct to pulmonary preservation with euro-collins solution. | the addition of prostaglandin to the single-flush technique of lung preservation is considered to enhance subsequent graft function. we have evaluated the use of the prostacyclin analog, iloprost, in an animal model of unilateral lung transplantation. group 1 (n = 5) received iloprost as a pretreatment intravenously (20 ng/kg/min) and as an additive (20 ng/l) to 20 ml/kg of modified euro-collins solution. group 2 (n = 5) received no iloprost, either as pretreatment or added to the perfusate. per ... | 1990 | 1690470 |
| the superiority of an extracellular fluid solution over euro-collins' solution for pulmonary preservation. | limited donor supply is the major factor restricting the application of lung transplantation. a uniformly reliable method of lung preservation would improve donor organ availability. at present, euro-collins' solution, an intracellular fluid-type solution, is most widely used in organ preservation. however, we have previously shown that initial pulmonary flush with an extracellular-type solution (low-potassium dextran solution [lpd]) provided better pulmonary preservation than euro-c. in the pre ... | 1990 | 1691536 |
| preservation of the porcine pancreas with htk and euro-collins solution: studies in a reperfusion system. | the present study compares the preservation of the porcine pancreas by the standard euro-collins solution or the cardioplegic histidine-tryptophan-ketoglutarate solution (htk). the explanted pancreas was stored at 4 degrees c for 6 and 24 h respectively, following which organ quality was assessed in a reperfusion chamber measuring physiological and biomedical parameters. after 6 h ischaemia, the amount of lactate was significantly lower when htk was used for protection. other parameters like ins ... | 1990 | 1702387 |
| effects of euro-collins, university of wisconsin, and histidine-tryptophane-ketoglutarate solution on hepatic microcirculation following liver transplantation in the rat. | | 1991 | 1899315 |
| calcium channel blockers for kidney protection. | acute postischemic renal failure (arf) is a major complication in surgery and in particular in renal transplantation. calcium channel blockers (ccbs) are able to prevent or ameliorate arf in different experimental models when given before ischemic injury. because arf, which is observed in 20-60% of graft recipients, carries the risk of undetected rejections and nephrotoxic injury and is also expensive, we tested the hypothesis, suggested by animal experiments, that there is also a beneficial eff ... | 1990 | 1707109 |
| the effect of low-potassium-dextran versus euro-collins solution for preservation of isolated type ii pneumocytes. | limited availability of donor organs is a major factor restricting the clinical application of lung transplantation. improvements in preservation techniques are essential for prolonging storage time and improving lung function following transplantation. the present investigation used primary cultures of adult rat alveolar type ii cells as a model for evaluating lung-preservation solutions. type ii cells were plated onto tissue-culture plastic at a density 5 x 10(5) cells/cm2 and maintained in du ... | 1991 | 1718065 |
| enhanced pulmonary function using dimethylthiourea for twelve-hour lung preservation. | current preservation techniques for lung transplantation limit ischemic time to 6 hours. the purpose of this study was to evaluate the ability of dimethylthiourea, a low molecular weight free radical scavenger, to prolong this interval. an in vivo canine transplantation model was used to assess lung function. at harvest and after circulatory arrest, the donor lung was flushed with modified euro-collins solution (50 ml/kg). in a blinded fashion, dimethylthiourea (5 g) or saline solution was added ... | 1991 | 1903917 |
| the effect of pge1 and temperature on lung function following preservation. | we studied the effect of a vasodilator (prostaglandin e1) as well as flush (f) and storage (s) temperatures (4 degrees c or 10 degrees c) on lung preservation in an isolated rabbit lung perfusion model. low-potassium dextran (lpd) or euro-collins (e-c) solution was used as flush solution. six groups of six animals were studied: group 1 (lpd, 4 degrees c f-s), group 2 (lpd with pge1, 4 degrees c f-s), group 3 (e-c with pge1, 4 degrees c f-s), group 4 (lpd, 10 degrees c f-s), group 5 (lpd with pge ... | 1991 | 1718066 |
| low-potassium uw solution for lung preservation. comparison with regular uw, lpd, and euro-collins solutions. | university of wisconsin solution has been used successfully in clinical kidney and liver preservation. the object of this study was to determine if low-potassium uw (lpuw) solution could be applied to pulmonary preservation. rabbit lungs were stored after hypothermic pulmonary artery (pa) flush with four different solutions (group 1: low-potassium dextran (lpd) solution, group 2: high-potassium uw (hpuw) solution, group 3: lpuw solution, group 4: modified euro-collins (e-c) solution). the lungs ... | 1991 | 1721252 |
| involvement of oxygen in harvesting injury of the liver. an experimental study including substrate free organ persufflation to evaluate a specific therapeutic approach. | the present study was undertaken to assess the role of oxygen free radicals relating to cell damage upon reoxygenation of the ischemically altered isolated rat liver. livers were excised and flushed via the portal vein with ringer's solution and euro-collins solution, to which superoxide dismutase (sod) was added in the experimental group. after warm ischemia at 37 degrees c and cold storage at 4 degrees c, the livers were reperfused via the portal vein with carbogen-saturated krebs-henseleit so ... | 1991 | 1925067 |
| [pancreatic conservation after autotransplantation in dogs. value of histologic surface analysis]. | a study of an artificial conservation fluid (hyperosmolar, ph = 8, rich in lactobionate and raffinose) was carried out by means of an experimental procedure involving segmental pancreatic autotransplants in dogs. the study covers 14 transplants, seven carried out without conservation and seven with 24-hour conservation at 4 degrees c. the caudal pancreas was removed after splenectomy and either transfused with 250 ml of 4 degrees c euro-collins before immediate transplant or with 250 ml of 4 deg ... | 1991 | 1721772 |
| reduction by superoxide dismutase of leukocyte-endothelial adherence after liver transplantation. | one hour after orthotopic rat liver transplantation, hepatic microcirculation and adhesion characteristics of leukocytes to the endothelial wall were studied with intravital microscopy. cold storage for 1 and 8 hours in euro-collins solution resulted in reduction of perfused sinusoids to 83% and 48% and a decrease of leukocyte velocity from 417 microns/sec in controls to 311 microns/sec and 269 microns/sec, respectively. additionally, the number of permanently adherent leukocytes rose from 4% in ... | 1992 | 1728081 |
| university of wisconsin versus modified euro-collins solution for lung preservation. | in a canine model, the quality of lung preservation was assessed using pulmonary artery flush after prostacyclin administration with either modified euro-collins solution or university of wisconsin solution. twelve combined heterotopic heart and orthotopic left lung allotransplantations were performed after 6 hours of cold ischemia. myocardial preservation was achieved using st. thomas hospital solution. donor organs were anastomosed parallel to the recipient's heart and right lung, and the supe ... | 1992 | 1728244 |
| glutathione decreases the pulmonary reimplantation response in canine lung autotransplants. | the pulmonary reimplantation response (prr) is a form of membrane permeability pulmonary edema occurring in lung transplants. the severity of the prr reflects the quality and duration of lung graft preservation. free radicals formed during ischemia with reperfusion in the autotransplanted dog lung may play a role in producing prr. we hypothesized that the addition of reduced glutathione (gsh) to the preservative solution could decrease prr if hydroperoxides are being formed. six dogs underwent l ... | 1991 | 1959416 |
| future horizons of lung preservation by application of a platelet-activating factor antagonist compared with current clinical standards. euro-collins flush perfusion versus donor core cooling. | with the introduction of platelet-activating factor antagonists, a direct inhibition of ischemia-induced reperfusion injury can be achieved by prevention of platelet activation, reduction of microvascular leakage, and platelet-activating factor-induced bronchoconstriction. at present, two preservation methods are established for clinical lung preservation: (1) donor core cooling by extracorporeal circulation and (2) pulmonary artery flush with euro-collins solution and prostacyclin. we compared ... | 1992 | 1735984 |
| correlation between high adenosine triphosphate tissue concentration and good posttransplant outcome for the canine pancreas graft after preservation by the two-layer cold storage method. | assessment of viability of a pancreas graft during preservation is very important to avoid transplantation of a nonfunctioning allograft. in the present report the correlation between adenosine triphosphate tissue concentration at the end of cold preservation by the two-layer method and viability a of canine pancreas graft following transplantation was studied. after preservation by an original two-layer (euro-collins' solution/perfluorochemical) method (group 1) and a modified two-layer (univer ... | 1991 | 1750085 |
| donor organ preservation fluids differ in their effect on endothelial cell function. | successful organ transplantation depends on adequate preservation of cellular function. we tested the effect of four commonly used donor organ preservation fluids on the ability of cultured bovine pulmonary artery endothelial cells to release endothelium-derived relaxing factor (edrf). columns of endothelial cells grown on microcarrier beads were exposed to either university of wisconsin (belzer's) solution, marshall's preservation fluid, euro-collins solution, or a blood-based preservation flui ... | 1991 | 1756167 |
| methyl palmitate prevents kupffer cell activation and improves survival after orthotopic liver transplantation in the rat. | the purpose of this study was to determine whether prevention of kupffer cell activation following orthotopic liver transplantation improves postoperative survival. first, particle phagocytosis by kupffer cells was monitored continuously from the uptake of colloidal carbon by the perfused liver. unstored livers took up carbon at rates of around 150 mg/g per hour, whereas storage for 24 h in euro-collins solution nearly doubled values to about 290 mg/g per hour. treatment of rats with methyl palm ... | 1991 | 1786059 |
| [primary hepatocyte cultures as a model of experimental study of liver preservation]. | primary hepatocyte cultures have been used to evaluate data concerning hypoxic liver cell injury. to show the suitability of this method in liver preservation studies hepatocyte cultures were incubated under different conditions: warm normoxia (37 degrees c, po2 greater than 70 mm hg), warm hypoxia (37 degrees c, po2 less than 0.1 mm hg), cold normoxia (4 degrees c, po2 greater than 70 mm hg) and cold hypoxia (4 degrees c, po2 less than 0.1 mm hg). incubations were performed in euro collins solu ... | 1991 | 1791732 |
| [morphologic analysis of damage in renal grafts in various preservative solutions]. | in this study experimental research was conducted concerning the morphology of renal lesions, in the function of time (12, 24, 36 and 48 hours) on potential renal allografts, which were stored in various cooled preservative fluids. a physiological solution was used, euro-collins and modified euro-collins, where dextrose was substituted with mannitol in the quantity of 35 g/l. with the modification of the euro-collins solution, in other words by the substitution of dextrose with mannitol, better ... | 1991 | 1808480 |
| euro-collins solution versus uw-solution for long-term liver preservation in the isolated rat-liver perfusion model. | to compare uw-solution (uw) and euro-collins (ec) for long-term liver preservation we investigated the morphology and metabolic capacity of rat liver after 18 and 42-hours cold-storage in either uw or ec. after harvesting the rat liver was transferred to a perfusion chamber where it was perfused for 10 min with uw or ec at 4 degrees c. thereafter livers were stored at 4 degrees c in uw or ec for 18 hours (both groups n = 6) or for 42 hours (both groups n = 8). after 18-hr or 42-hr cold-storage a ... | 1991 | 1810373 |
| adenine nucleotides metabolism of the canine pancreas during preservation by the two-layer cold storage method. | the relationship between tissue concentrations of adenine nucleotides (ans) at the end of cold preservation period and organ viability after transplantation is controversial. the purpose of this study is to examine energy metabolism of the pancreas graft preserved by a two-layer cold storage method and the relationship with the viability of the pancreas graft following transplantation. after preservation by simple cold storage with euro-collins' solution (ec) (group 1) or university of wisconsin ... | 1991 | 1817204 |
| preservation of skeletal muscle in tissue transfers using rat hindlimbs. | replantation of major extremities after long periods of ischemia can lead to viable replants in many cases, but functional restoration is often poor owing to fibrosis of the muscle. in this study, maximum hypothermic time in tissue transfers containing skeletal muscle using hindlimbs of lewis rats preserved in 4 degrees c euro-collins solution was investigated. after preserving midthigh amputated legs in this solution for 6, 9, and 12 hours, the legs were transplanted to other inbred rats using ... | 1991 | 1852821 |
| tumor necrosis factor and endotoxin in the pathogenesis of liver and pulmonary injuries after orthotopic liver transplantation in the rat. | this study examines whether tumor necrosis factor and endotoxin are involved in the pathogenesis of primary nonfunction of graft and pulmonary complication after orthotopic liver transplantation. livers from lewis rats were stored for either 1 or 4 hr in ice-cold euro-collins solution (1-hr storage and 4-hr storage group, respectively). subsequently, donor livers were implanted orthotopically. in some experiments, anti-tumor necrosis factor antibody was administered intravenously before and imme ... | 1992 | 1639357 |
| trace element reference values in tissues from inhabitants of the european community. ii. examples of strategy adopted and trace element analysis of blood, lymph nodes and cerebrospinal fluid of italian subjects. | the euro terviht (trace element reference values in human tissues), recently initiated, aims to establish and compare trace metal reference values in inhabitants from the different ec countries. the project anticipates international cooperation of specialized chemical and toxicological laboratories in western europe. in order to overcome the well known and intolerable high fluctuation in published trace metal concentrations in body fluids and tissues, which are mostly due to poor analysis, this ... | 1992 | 1641639 |
| [an evaluation method for organ preservation]. | the determination of the active transport of alanine by canine endothelial cells was employed as a parameter to test cellular viability following conservation in vitro at 4 degrees c in krebs bicarbonate buffer (kb), st. thomas (st), euro-collins (ec) or university of wisconsin (uw) solutions. following 24-hour conservation, active transport values demonstrated that the highest results were obtained with uw solution. at the same time, dog renal cortex slices were also tested under identical cond ... | 1992 | 1644615 |
| fluorometric study of the viability of rat liver grafts after simple cold storage with uw solution versus euro-collins solution. | | 1991 | 2053182 |
| cold ischemic damage to bone. | to clarify changes in bone tissue and osteogenetic ability after preservation under conditions of cold ischemic, a series of experiments were carried out using a rat model. animals were grouped as follows: a group of 11 rats whose amputated legs were preserved in euro-collins solution at 4 degrees c for 24 hr and then transferred to the corresponding site of a different genetically similar rat; a group of seven animals whose amputated legs were preserved for 48 hr under the same conditions and t ... | 1991 | 1890675 |
| improved method of porcine renal allografting for transplantation research. | this study presents a refined, reproducible, and clinically appropriate animal model of renal transplantation. a pair of kidneys are harvested from a donor pig and preserved in euro-collins' solution (4 degrees c). after a set period of preservation, the allografts are transplanted to two recipient pigs. the abdomen is entered through a midline incision. the right common iliac artery and vein are dissected and bilateral native nephrectomy is performed. each allograft is then randomly assigned an ... | 1991 | 2069932 |
| high potassium contents in organ preservation solutions cause strong pulmonary vasocontraction. | euro-collins (ecs) and ucla-formula organ preservation solutions induced strong vasocontraction in porcine pulmonary arteries when studied in organ baths at temperatures of 37 degrees c and 30 degrees c. at 20 degrees c ecs induced a 30% contraction, but at 6 degrees c no contraction (n = 5) or a weak contraction (n = 1) was elicited. neither prostaglandin e1 nor nifedipine caused any significant reduction of the vasocontraction elicited by ecs and ucla. krebs solution, enriched with potassium i ... | 1991 | 1898141 |
| cold preservation injury of nadh-ubiquinone oxidoreductase. | mitochondrial injury caused by cold preservation without oxygenation was studied polarographically. respiration activity with glutamate as substrate was impaired after 6 hours preservation with euro-collin's solution at 4 degrees c, while that with succinate as substrate was maintained at the control level after 24 hours preservation. membrane potential across mitochondrial membrane in state 4 was not damaged after 24 hours preservation. these results indicate that nadh-ubiquinone oxidoreductase ... | 1991 | 1900556 |
| rapid increase in the activity of enzymes of eicosanoid synthesis in hepatic and extrahepatic tissues after experimental liver transplantation. | previous studies have demonstrated a marked release of prostanoids from hepatic tissue after liver grafting. in addition, eicosanoid synthesis was shown to be regulated at the level of key enzymes. the present study addressed changes of the local availability of these enzymes during and after porcine orthotopic liver transplantation. we determined kinetic parameters of cyclooxygenase (co), the initial enzyme of prostaglandin synthesis, of prostacyclin and thromboxane synthase (pcs, txs), two mor ... | 1991 | 1903222 |
| six-hour porcine liver storage without flushing or perfusion. | livers from normal porcine donors were preserved by surface cooling only, without flushing or perfusion, for periods up to 24 hr. all recipients of livers stored for 6 hr survived until sacrifice at 7 days. in a separate, similar group, survival up to 21 days was noted. only 2 of 6 recipients survived after 9-hr liver storage, but one of these lived for greater than 120 days. no animals survived longer than 2 days after transplantation of livers stored for 12 or 24 hr. the changes in plasma leve ... | 1991 | 1907044 |
| influence of glycogen content, temperature, and euro collins solution on membrane potential and sodium activity of superfused porcine liver slices. | the influence of glycogen content, temperature, and euro collins (ec) solution on membrane potential (vm) and intracellular sodium activity (anai) were measured in cells of superfused porcine liver slices by means of double-barrelled ion-sensitive microelectrodes. vm was -26.1mv in fasted pigs and -20.6mv after glucose feeding, when measured in hepes-buffered solution (p less than 0.0001). anai was not measurably affected by glucose feeding. during superfusion with tyrode solution, lowering the ... | 1991 | 1925066 |
| prolonged hypothermic cardiac storage with university of wisconsin solution. an assessment with human cell cultures. | hypothermic storage of cardiac allografts is routinely used for transplantation but is associated with an increased mortality when ischemic times are greater than 4 hours. the ideal storage conditions (solution and temperature) could extend the current limits of cold ischemia. human endothelial cells and ventricular myocytes were studied to screen various solutions and temperatures for organ preservation. four solutions (modified euro-collins, phosphate-buffered saline, stanford cardioplegia, an ... | 1991 | 1943184 |
| evaluation of solutions for small intestinal preservation. biochemical changes as a function of storage time. | a number of organ preservation solutions have been formulated to slow the inevitable progression of ischemic injury, thus prolonging the storage time between removal and implantation. as adenine nucleotide content has been shown to correlate with the functional recovery of transplanted livers and hearts, this study investigated the effects of 24 hr of storage in three preservation solutions, saline (sa), euro-collins (co), and university of wisconsin (uw) on adenine and nicotinamide adenine nucl ... | 1991 | 1949162 |
| a strategy to increase the donor pool: use of cadaver lungs for transplantation. | a shortage of suitable donors is a serious obstacle to the widespread application of isolated lung transplantation for end-stage lung disease. we hypothesized that lung tissue likely remains viable for a sufficient period of time to allow for safe postmortem retrieval of lungs for transplantation. studies were conducted in a nonsurvival model of canine lung allotransplantation. donor animals were sacrificed, and subsequent lung harvest was delayed for 1 hour, 2 hours, or 4 hours. pulmonary retri ... | 1991 | 1953132 |
| kupffer cell activation and endothelial cell damage after storage of rat livers: effects of reperfusion. | reperfusion injury characterized by loss of endothelial cell viability occurs after cold ischemic storage of livers for transplantation surgery. here, ultrastructural changes in stored rat livers were examined by scanning and transmission electron microscopy. with increasing times of storage in euro-collins solution (4 to 24 hr) followed by 15 min of reperfusion at 37 degrees c, a sequence of structural alterations was observed involving endothelial and kupffer cells. widening of endothelial fen ... | 1991 | 1988348 |
| islet purification using euro-ficoll gradients. | | 1991 | 1990686 |
| twenty-four-hour canine lung preservation using uw solution. | the left lungs of 14 mongrel dogs were isolated, preserved, and then reperfused for 120 min. three groups of lungs were investigated: group 1, nonpreserved lungs (control n = 5); group 2, lungs were flushed with uw solution and cold-stored (4-6 degrees c) in the same flush solution for 24 hr (n = 4); and group 3, lungs flushed and cold-stored with modified euro-collins' solution for 24 hr (n = 5). airway pressure (awp), static lung compliance (cst), and pulmonary vascular resistance (pvr) 120 mi ... | 1991 | 2006512 |
| long-term hypothermic storage of the cardiac explant. comparison of four solutions. | this study compared the capabilities of 4 storage solutions in protecting the cardiac explant. isolated rat heart was flushed with and stored in one of the storage solutions at 0 degrees c for 7 hours. the recovery of function was assessed using the working heart perfusion. cardiac output returned to 34 +/- 6, 59 +/- 3 76 +/- 5, and 75 +/- 8% (mean +/- se) of the control level in hearts stored in euro-collins, st. thomas' hospital cardioplegic, a modified university of wisconsin (muw) solutions, ... | 1991 | 2010446 |
| an assessment of crystalloid solutions for donor heart preservation. | the optimal technique for donor heart protection remains controversial. one component of preservation is the transport solution. although saline solution is most frequently used as a transport medium, other crystalloid solutions may be superior. accordingly, human right atrial trabeculae contracting isometrically in vitro were used to assess five crystalloid solutions at two different temperatures (12 degrees and 4 degrees c): st. thomas' hospital solution, modified krebs-henseleit (k+ = 16 meq/ ... | 1991 | 2023439 |
| prolonged preservation of canine lung allografts: the role of prostaglandins. | the role of prostaglandin e1 (pge1) and prostacyclin in enhancing the ischemic tolerance of single-lung grafts was investigated. fifteen donor dogs underwent pulmonary artery flushing with 60 ml/kg of 4 degrees c modified euro-collins solution; 5 dogs each received a 15-minute infusion of pge1, prostacyclin, or saline solution before flushing. after 12 hours of storage at 4 degrees c, left lung transplantation was performed in 15 recipient dogs. measurements were performed after 10 minutes of ri ... | 1991 | 2025103 |
| [the effect of prostaglandin i2 on the postischemic recovery of the transplanted heart under deep hypothermia]. | the effect of prostaglandin i2 (pgi2) on the postischemic recovery of the heart orthotopically transplanted under deep hypothermia was evaluated using puppies. twenty-four pairs of dogs were divided into 4 groups according to the usage of pgi2 and preservation of the donor heart (in euro-collins' solution at 4 degrees c for 4 hours), namely group i (non pgi2, no preservation), group ii (pgi2-treated, no preservation), group iii (non pgi2, 4 hours preservation), group iv (pgi2-treated, 4 hours pr ... | 1991 | 2026915 |
| hypertonic citrate solution as an alternative to modified euro-collins' solution for lung preservation. | in a canine model of acute ischemic lung injury, a hypertonic citrate solution (htc) widely used for renal preservation in the united kingdom, was compared with modified euro-collins' solution (ecs) currently the most widely clinically used pulmonary perfusate. ten beagle dogs underwent left thoracotomy and exclusion of the left lung in situ. the lung was flushed with 30 ml/kg of either htc or ecs and subjected to 60 min of warm ischemia. the circulation to the lung was then restored, the contra ... | 1991 | 2031260 |
| a case of tularemia meningitis in sweden. | tularemia meningitis occurred in a previously healthy 64-year-old man from northern sweden. the patient was immunocompetent and recovered without sequelae. the bacterial isolate conformed by most criteria with the euro-asian biovar francisella tularensis var. palaearctica. tularemia meningitis may mimic meningitis caused by mycobacterium tuberculosis. | 1990 | 2320968 |
| leukocyte adhesion and cell death following orthotopic liver transplantation in the rat. | the purpose of these experiments was to employ video microscopy to identify early pathological changes after orthotopic liver transplantation in the rat. liver transplantation was performed using the cuff technique. survival was greater than 90% when livers were stored for 1 hr in ringer's solution prior to transplantation (survival conditions), whereas all rats died following storage of grafts for 4 hr in cold euro-collins solution (nonsurvival conditions). postoperatively, each recipient anima ... | 1991 | 2031279 |
| iron chelation therapy and lung transplantation. effects of deferoxamine on lung preservation in canine single lung transplantation. | reperfusion injury is a limiting factor in lung transplantation. deferoxamine is an iron chelator that inhibits the formation of oxygen-derived free radicals. we investigated the effects of deferoxamine on posttransplantation lung function in a canine model of single lung transplantation. twelve dogs underwent left lung transplantation after 20- to 24-hour hypothermic storage in a modified euro-collins solution. in six experiments donor and recipient received a 10 mg/kg dose of deferoxamine befo ... | 1991 | 2038195 |
| automated large-scale isolation, in vitro function and xenotransplantation of porcine islets of langerhans. | to evaluate the potential of utilizing porcine islet tissue as an alternative to human islet tissue for transplantation, we developed a method for the isolation of large amounts of highly purified porcine islets, and assessed the in vitro and in vivo function of the isolated islets after 1, 4, and 7 days of culture. the pancreatic duct of the splenic lobe was cannulated and distended by injection of hanks' balanced salt solution containing 1.5 mg/ml collagenase. the pancreas was then processed b ... | 1991 | 1871791 |
| increase in survival of liver grafts after rinsing with warm ringer's solution due to improvement of hepatic microcirculation. | temperature increases membrane fluidity and decreases vascular resistance in isolated organs. therefore, these studies were designed to determine if a rinse with warm buffer could increase survival time in the rat model of orthotopic liver transplantation by improving hepatic microcirculation. brief periods of warm ischemia (3-8 min) did not damage the liver as indexed by minimal release of ldh. survival of rats for 30 days was greater than 90% in this model when livers were stored for 1 hr in r ... | 1991 | 1871793 |
| the development of a community-based hiv/aids counselling service in a rural area in uganda. | this paper describes the evolution of a counselling service in the context of an hiv/aids research programme in rural uganda. the background to aids counselling both in europe/america and in africa is outlined first, and the cultural context of the programme explained. the evolution of the counselling procedure is then described and village case studies are used to illustrate the development and the difficulties that have been faced in finding an appropriate counselling model. it is noted that t ... | 1991 | 1878404 |
| effect of verapamil on conversion of xanthine dehydrogenase to oxidase in ischemic rat liver. | the effect of organ flushing with the calcium entry blocker verapamil on the conversion of innocent enzyme xanthine dehydrogenase (xdh) to superoxide generating enzyme xanthine oxidase (xod) in ischemic rat livers was studied. this enzyme conversion progressed over time in warm or cold ischemia. in non-flushed livers, the activities of xod as percentages of xdh plus xod after 6 h at 37 degrees c and 6 days at 4 degrees c were 80.3 +/- 5.2 and 31.6 +/- 2.1, respectively. in the livers flushed wit ... | 1990 | 2089535 |
| glycogen effects on energy state and passive electric properties of liver during protection. | in order to evaluate the importance of glycogen for the hepatic tolerance to ischemia, livers of swine fed a glucose-potassium solution for premedication were perfused with either bretschneider's htk-solution (histidine-tryptophan-ketoglutarate) or with euro-collins-solution (ec) prior to subsequent ischemia at 25 and 5 degrees c. during ischemia, in regular intervals or continuously, energy rich phosphates, lactate, intrahepatic ph and the electrical impedance of liver tissue were determined. t ... | 1990 | 2111613 |
| amelioration of lung ischemic injury with prostacyclin. | the single-flush technique of lung preservation is thought to be enhanced by prostaglandin treatment. in order to test this hypothesis, ten beagle dogs underwent thoracotomy and in situ flush perfusion of the excluded left lung with 30 ml/kg of cold, modified euro-collins' solution. group 1 (n = 5) received pretreatment with 30 ng/kg/min of pgi2 by infusion and as an additive to the flush (20 micrograms/l). group 2 (n = 5) received no pgi2 and served as controls. following 60 min of warm ischemi ... | 1990 | 2113724 |