| pepo aphid-borne yellows virus: a new species in the genus polerovirus. | pepo aphid-borne yellows virus (pabyv) has been proposed as a putative representative of a new species in the genus polerovirus in the family luteoviridae. the genomes of two south african (sa) isolates of cucurbit-infecting pabyv were described in this record. total rna, extracted from a pattypan (cucurbita pepo l.) and a baby marrow (c. pepo l.) leaf samples, was subjected to next-generation sequencing (ngs) on the hiseq illumina platform. sanger sequencing was subsequently used to authenticat ... | 2017 | 27623838 |
| molecular diversity of poleroviruses infecting cucurbit crops in four countries reveals the presence of members of six distinct species. | when 66 cucurbit samples with yellowing symptoms from fields in mali, the philippines, thailand and uzbekistan were screened by rt-pcr using universal polerovirus primers, 21 were identified as harboring polerovirus rna. when these 21 samples were screened with specific primers for the known cucurbit-infecting poleroviruses, suakwa aphid-borne yellows virus and a recombinant strain of cucurbit aphid-borne yellows virus were detected for the first time in the philippines and thailand. however, se ... | 2014 | 24337720 |
| new poleroviruses associated with yellowing symptoms in different vegetable crops in greece. | four poleroviral isolates from greece, two from lettuce, one from spinach and one from watermelon showing yellowing symptoms, were molecularly characterized by analyzing the sequence of a large part of the genome spanning from the 3'-terminal part of the rdrp to the end of the cp gene. the sequences were analyzed for their similarity and phylogenetic relationships to other members of the genus polerovirus as well as for evidence of recombination events. the results revealed the existence of two ... | 2016 | 26530834 |