incidence and molecular diversity of poleroviruses infecting cucurbit crops and weed plants in thailand. | overall, 244 samples of cucurbit crops with yellowing symptoms and selected weed species, from 15 provinces in thailand, were screened by rt-pcr using primers polero-cp-f and polero-cp-r. a total of 160 samples (~66%) were infected by poleroviruses. analysis of a 1.4 kb region covering the 3' rna-dependent rna polymerase (rdrp) gene, the intergenic non-coding region (incr), and the coat protein (cp), showed that four poleroviruses, namely, cucurbit aphid-borne yellows virus (cabyv), luffa aphid- ... | 2017 | 28352973 |
molecular diversity of poleroviruses infecting cucurbit crops in four countries reveals the presence of members of six distinct species. | when 66 cucurbit samples with yellowing symptoms from fields in mali, the philippines, thailand and uzbekistan were screened by rt-pcr using universal polerovirus primers, 21 were identified as harboring polerovirus rna. when these 21 samples were screened with specific primers for the known cucurbit-infecting poleroviruses, suakwa aphid-borne yellows virus and a recombinant strain of cucurbit aphid-borne yellows virus were detected for the first time in the philippines and thailand. however, se ... | 2014 | 24337720 |
first full-length genome sequence of the polerovirus luffa aphid-borne yellows virus (labyv) reveals the presence of at least two consensus sequences in an isolate from thailand. | luffa aphid-borne yellows virus (labyv) was proposed as the name for a previously undescribed polerovirus based on partial genome sequences obtained from samples of cucurbit plants collected in thailand between 2008 and 2013. in this study, we determined the first full-length genome sequence of labyv. based on phylogenetic analysis and genome properties, it is clear that this virus represents a distinct species in the genus polerovirus. analysis of sequences from sample th24, which was collected ... | 2015 | 26195192 |