| novel flaviviruses detected in different species of mosquitoes in spain. | abstract we report the characterization of three novel flaviviruses isolated in spain. marisma mosquito virus, a novel mosquito borne virus, was isolated from ochlerotatus caspius mosquitoes; spanish ochlerotatus flavivirus and spanish culex flavivirus, two novel insect flaviviruses, were isolated from oc. caspius and culex pipiens, respectively. during this investigation, we designed a sensitive rt-nested polymerase chain reaction method that amplifies a 1019bp fragment of the flavivirus ns5 ... | 2011 | 22022811 |
| molecular characterization of flaviviruses from field-collected mosquitoes in northwestern italy, 2011-2012. | the genus flavivirus comprises several mosquito-borne species, including the zoonotic pathogens west nile and usutu virus, circulating in animals and humans in italy since 1998. due to its ecological and geographical features, piedmont is considered a risk area for flavivirus transmission. here we report the results of a flavivirus survey (detection and genetic characterization) of mosquitoes collected in piedmont in 2012 and the genetic characterization of three strains detected in 2011. | 2014 | 25160565 |
| complete genome sequences of two insect-specific flaviviruses. | we determined the complete genomic sequences of two previously discovered insect-specific flaviviruses, marisma mosquito virus (mmv) and nanay virus (nanv), using a combination of high-throughput sequencing, reverse transcription-polymerase chain reaction, 5' and 3' rapid amplification of cdna ends and sanger sequencing. complete polyprotein amino acid sequence alignments revealed that the closest known relatives of mmv and nanv are donggang virus (89% identity, 95% similarity) and nounané virus ... | 2017 | 28918473 |