| novel insect-specific flavivirus isolated from northern europe. | mosquitoes collected in finland were screened for flaviviral rna leading to the discovery and isolation of a novel flavivirus designated hanko virus (hankv). virus characterization, including phylogenetic analysis of the complete coding sequence, confirmed hankv as a member of the "insect-specific" flavivirus (isf) group. hankv is the first member of this group isolated from northern europe, and therefore the first northern european isf for which the complete coding sequence has been determined. ... | 2012 | 22999256 |
| co-circulation of west nile virus and distinct insect-specific flaviviruses in turkey. | active vector surveillance provides an efficient tool for monitoring the presence or spread of emerging or re-emerging vector-borne viruses. this study was undertaken to investigate the circulation of flaviviruses. mosquitoes were collected from 58 locations in 10 provinces across the aegean, thrace and mediterranean anatolian regions of turkey in 2014 and 2015. following morphological identification, mosquitoes were pooled and screened by nested and real-time pcr assays. detected viruses were f ... | 2017 | 28320443 |
| characterization of an insect-specific flavivirus (ocfvpt) co-isolated from ochlerotatus caspius collected in southern portugal along with a putative new negev-like virus. | we describe the isolation and characterization of an insect-specific flavivirus (isf) from ochlerotatus caspius (pallas, 1771) mosquitoes collected in southern portugal. the rna genome of this virus, tentatively designated ocfvpt, for o. caspius flavivirus from portugal, encodes a polyprotein showing all the features expected for a flavivirus. as frequently observed for isf, the viral genomes seems to encode a putative fairly interesting flavivirus orf (fifo)-like product, the synthesis of which ... | 2013 | 23877720 |
| insect-specific flaviviruses in aedes mosquitoes in greece. | mosquitoes of the genus aedes are known vectors of pathogenic flaviviruses, and insect-specific flaviviruses (isfs) have been detected in members of this genus in numerous parts of the world. in order to gain insight into whether aedes mosquitoes in greece are infected by flaviviruses, 1173 aedes spp. mosquitoes collected in 2010 and 2012 were grouped in 53 pools and tested by rt nested pcr using flavivirus generic primers. eight pools (15.09 %) were found to be pcr positive: five pools (5/53, 9 ... | 2016 | 27198866 |
| a novel insect-specific flavivirus replicates only in aedes-derived cells and persists at high prevalence in wild aedes vigilax populations in sydney, australia. | to date, insect-specific flaviviruses (isfs) have only been isolated from mosquitoes and increasing evidence suggests that isfs may affect the transmission of pathogenic flaviviruses. to investigate the diversity and prevalence of isfs in australian mosquitoes, samples from various regions were screened for flaviviruses by elisa and rt-pcr. thirty-eight pools of aedes vigilax from sydney in 2007 yielded isolates of a novel flavivirus, named parramatta river virus (parv). sequencing of the viral ... | 2015 | 26519596 |