phylogenetic analysis of a novel molecular isolate of spotted fever group rickettsiae from northern peru: candidatus rickettsia andeanae.phylogenetic analysis of five rickettsial genes (17-kda gene, glta, ompb, ompa, and sca4) from two molecular isolates of candidatus rickettsia andeanae from two ticks (amblyomma maculatum and ixodes boliviensis) collected from two domestic horses living in two separate locations in northern peru (coletas and naranjo) was conducted to more clearly characterize this recently reported novel spotted fever group (sfg) rickettsia. following nested polymerase chain reaction (pcr) amplification of the 1 ...200516481537
characterization of spotted fever group rickettsiae in flea and tick specimens from northern peru.evidence of spotted fever group (sfg) rickettsiae was obtained from flea pools and individual ticks collected at three sites in northwestern peru within the focus of an outbreak of febrile disease in humans attributed, in part, to sfg rickettsia infections. molecular identification of the etiologic agents from these samples was determined after partial sequencing of the 17-kda common antigen gene (htra) as well as pairwise nucleotide sequence homology with one or more of the following genes: glt ...200415528680
[relative abundance of amblyomma spp. (acari: ixodidae) in bovines (bos taurus and b. indicus) from costa rica].the research describe the big amount of ticks of the amblyomma genus, found on bovines through monthly samplings carried out in ten farms in eight ecological zones (ez) of costa rica. ticks larger than 4 mm were picked up from the right side of the animals during the visit. the study compiled meteorological information for some farms located in the experiment, showing that the most fluctuant variable is rainfall. the most important amblyomma species found was a. cajennense. amblyomma nymphs were ...200315162737
distribution of spotted fever group rickettsiae in hard ticks (ixodida: ixodidae) from panamanian urban and rural environments (2007-2013).tick-borne rickettsiosis is an important emerging disease in panama; to date, there have been 12 confirmed cases, including eight fatalities. to evaluate the distribution of rickettsiae in panamanian ticks, we collected questing and on-host ticks in urban and rural towns in elevations varying between 0 and 2300 m. a total of 63 sites (13 urban and 50 rural towns) were used to develop models of spatial distributions. we found the following tick species: rhipicephalus sanguineus s.l. (present in 5 ...201627068930
exposure of dogs to spotted fever group rickettsiae in urban sites associated with human rickettsioses in costa rica.the zoonotic transmission cycles of rickettsia rickettsii and other spotted fever group (sfg) rickettsiae in latin america have usually been associated with rural or sylvatic environments, although domestic dogs can be implicated in more populated settings. in this study, exposure of dogs to sfg rickettsiae in the greater metropolitan area of costa rica was investigated. dogs from sites associated with human cases and from dog shelters were evaluated by indirect immunofluorescence assay (ifa) us ...201626995323
molecular detection and identification of rickettsiales pathogens in dog ticks from costa rica.although vector-borne diseases are globally widespread with considerable impact on animal production and on public health, few reports document their presence in central america. this study focuses on the detection and molecular identification of species belonging to selected bacterial genera (ehrlichia, anaplasma and rickettsia) in ticks sampled from dogs in costa rica by targeting several genes: 16s rrna/dsb genes for ehrlichia; 16s rrna/groel genes for anaplasma, and ompa/glta/groel genes for ...201627480409
investigation of tick-borne bacteria (rickettsia spp., anaplasma spp., ehrlichia spp. and borrelia spp.) in ticks collected from andean tapirs, cattle and vegetation from a protected area in ecuador.ixodid ticks play an important role in the transmission and ecology of infectious diseases. information about the circulation of tick-borne bacteria in ticks is lacking in ecuador. our aims were to investigate the tick species that parasitize andean tapirs and cattle, and those present in the vegetation from the buffer zone of the antisana ecological reserve and cayambe-coca national park (ecuador), and to investigate the presence of tick-borne bacteria.201525616567
in vitro propagation of candidatus rickettsia andeanae isolated from amblyomma maculatum.candidatus rickettsia andeanae was identified during an investigation of a febrile outbreak in northwestern peru (2002). dna sequencing from two ticks (amblyomma maculatum, ixodes boliviensis) collected during the investigation revealed a novel rickettsia agent with similarity to the spotted fever group rickettsiae. since then, candidatus r. andeanae has been detected in a. maculatum ticks collected in the southeastern and southcentral united states, argentina, and peru. to date, candidatus r. a ...201122098316
comments on controversial tick (acari: ixodida) species names and species described or resurrected from 2003 to 2008.there are numerous discrepancies in recent published lists of the ticks of the world. here we review the controversial names, presenting evidence for or against their validity and excluding some altogether. we also address spelling errors and present a list of 17 species described or resurrected during the years 2003-2008. we consider the following 35 tick species names to be invalid: argas fischeri audouin, 1826, ornithodoros boliviensis kohls and clifford, 1964, ornithodoros steini (schulze, 1 ...200919169832
ticks and tick-borne pathogens of dogs along an elevational and land-use gradient in chiriquí province, panamá.systematic acarological surveys are useful tools in assessing risk to tick-borne infections, especially in areas where consistent clinical surveillance for tick-borne disease is lacking. our goal was to identify environmental predictors of tick burdens on dogs and tick-borne infectious agents in dog-derived ticks in the chiriquí province of western panama to draw inferences about spatio-temporal variation in human risk to tick-borne diseases. we used a model-selection approach to test the relati ...201728417248
detection of an undescribed rickettsia sp. in ixodes boliviensis from costa rica.ixodes boliviensis is a tick of carnivores that is common on domestic dogs. the only rickettsia that has been detected previously in this species is 'candidatus rickettsia andeanae'. we report the detection of an undescribed rickettsia sp., named strain ibr/crc, in i. boliviensis collected from dogs in costa rica. analyses of glta, ompa, and htra partial sequences place rickettsia sp. strain ibr/crc in the group of r. monacensis, also close to an endosymbiont of ixodes scapularis and other undes ...201425132535
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