Publications

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parasites from the asiatic black bear (ursus thibetanus) on kyushu island, japan.two dirofilaria ursi, six trichodectes pinguis and numerous haemaphysalis megaspinosa were obtained from an asiatic black bear (ursus thibetanus) shot in kyushu, japan. the presence of two parasites specific to black bears may indicate the existence of a wild population, although the bears are regarded to be extinct on kyushu island.19902304196
studies on ixodid ticks. x. haemaphysalis megaspinosa n. sp. (ixodoidea, ixodidae) from kanagawa prefecture, japan. 19695821680
lyme disease spirochetes in ticks from northeastern china.during may 1996, field surveys on lyme disease spirochetes were conducted in beijing, shenyang, fushun, and inner mongolia in northeastern china. the ticks collected consisted of 3 genera and 12 species. of these, ixodes persulcatus was dominant in sun-exposed vegetation in forests in inner mongolia; 57 borrelia strains (55/123 unfed adults and 2/5 immature stages fed on a rodent) were obtained from this tick by bsk culture. additionally, 2/2 apodemus peninsulae were positive. ixodes nipponensis ...19989645846
tick bite by haemaphysalis megaspinosa - first case.we describe the first case of tick bite by haemaphysalis megaspinosa. the tick was found on the skin at the right occipital area in a 5-year-old girl, who had gone on a picnic twice to a mountain 1 and 5 days before she noticed the tick. the tick was surgically removed. the tick was identified as an adult female of haemaphysalis megaspinosa from its morphological characteristics. this is the first report of tick bite by haemaphysalis megaspinosa.200010882949
survey of ixodid tick species on domestic cats in japan.various species of ixodid ticks, attached to domestic cats in japan, were identified in spring (april to june) and autumn (september to november). in the spring, a total of 282 ticks, including 61 larvae, 70 nymphs, 127 females and 24 males were collected from 126 cats. of these, 264 were identified up to the species level. in the spring, haemaphysalis longicornis was the most frequently (39.7%, 50/126) found tick species on feline hosts, followed by ixodes ovatus (35.0%, 44/126), ixodes nippone ...200312531297
interspecific and intraspecific associations of two species of hard ticks, haemaphysalis longicornis and haemaphysalis megaspinosa, in relation to questing site.the effects of intraspecific and interspecific interactions on preferred questing sites of ticks, specifically nymphs and larvae of haemaphysalis longicornis and haemaphysalis megaspinosa, were examined in boso peninsula of central japan from october 1996 to september 1999. haemaphysalis longicornis were primarily segregated from h. megaspinosa by season. all stages of the 2 tick species preferred sedges. three-way contingency tables and log-linear models were used to test for independence of oc ...200717626344
detection of anaplasma bovis and anaplasma phagocytophilum dna from haemaphysalis megaspinosa in hokkaido, japan.dna from 101 ticks, including haemaphysalis megaspinosa, haemaphysalis douglasi, haemaphysalis flava, ixodes ovatus and ixodes persulcatus, collected by flagging in a pasture in hidaka district (hokkaido, japan) was examined for infection with anaplasma bovis and a. phagocytophilum by species-specific nested pcr. of the 48 h. megaspinosa nymphs, examined, 1 (2.1%) and 6 (12.5%) were positive for a. bovis and a. phagocytophilum, respectively. one of the 6 positive nymphs was dual positive for bot ...201019897306
isolation of the rickettsial agent genetically similar to candidatus rickettsia kotlanii, from haemaphysalis megaspinosa in japan.two rickettsial isolates, hm-1 and hm-2, were isolated from haemaphysalis megaspinosa collected in japan in 2006 and 2011, respectively. the isolates were analyzed by dna sequences of the outer membrane protein a gene, the outer membrane protein b gene, the citrate synthase gene, the genus rickettsia-specific outer membrane protein 17-kda gene, the 16s ribosome rna gene, and the ps120 protein gene ("gened"). hm-1 was identified as rickettsia tamurae. hm-2 matched most closely with 'candidatus ri ...025229707
survey on tick-borne pathogens in thoroughbred horses in the hidaka district, hokkaido, japan.a total of 87 thoroughbred horses and 10 ixodid ticks from a ranch in hidaka district, hokkaido were tested for tick-borne diseases. using the indirect fluorescent antibody (ifa) method, 3.4, 92.0 and 97.7% of the horses showed antibody titers of ≥ 80 against anaplasma phagocytophilum, rickettsia helvetica, and borrelia garinii, respectively. this is the first report of infection with the 3 pathogens in horses in japan. using pcr, dnas from the peripheral blood of all horses were found negative ...201322878540
genetic diversity of theileria orientalis in tick vectors detected in hokkaido and okinawa, japan.in the present study, we investigated the possible tick vectors that can transmit theileria orientalis in eastern hokkaido, japan. questing ticks collected from three different districts, taiki, otofuke, and shin-hidaka, of hokkaido included ixodes persulcatus, haemaphysalis megaspinosa, haemaphysalis douglasi, and ixodes ovatus, while all the ticks collected from yonaguni island of okinawa were identified as haemaphysalis longicornis. when the ticks were screened by polymerase chain reaction (p ...201222824416
detachment of hard ticks (acari: ixodidae) from hunted sika deer (cervus nippon).ixodid ticks were collected from 13 sika deer, cervus n.nippon, shot in the boso peninsula in central japan from late february to early march 1999. haemaphysalis megaspinosa was the most abundant species of the adults collected, although haemaphysalis longicornis, h. flava, h. kitaokai, h. cornigera, ixodes ovatus, and amblyomma testudinarium were also collected. males were more abundant than females for h. longicornis, h. megaspinosa, h. flava, and h. kitaokai. ticks that had inserted their hyp ...201424659518
epidemiological study of relapsing fever borreliae detected in haemaphysalis ticks and wild animals in the western part of japan.the genus borrelia comprises arthropod-borne bacteria, which are infectious agents in vertebrates. they are mainly transmitted by ixodid or argasid ticks. in hokkaido, japan, borrelia spp. were found in deer and haemaphysalis ticks between 2011 and 2013; however, the study was limited to a particular area. therefore, in the present study, we conducted large-scale surveillance of ticks and wild animals in the western part of the main island of japan. we collected 6,407 host-seeking ticks from two ...201728362864
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