| detection of rickettsia japonica in haemaphysalis longicornis ticks by restriction fragment length polymorphism of pcr product. | pcr was applied to the detection of rickettsia japonica, the causative agent of oriental spotted fever (osf), in ticks collected at two sites of the muroto area on shikoku island, a major area in japan where osf is endemic. primer pair rr190.70p and rr190.602n of the r. rickettsii 190-kda antigen gene sequence of regnery and others (r.l. regnery, c.l. spruill, and b.d. plikaytis, j. bacteriol. 173:1576-1589, 1991) primed the dna extracted from haemaphysalis longicornis ticks but not those extrac ... | 1995 | 7790445 |
| detection of ehrlichia spp., anaplasma spp., rickettsia spp., and other eubacteria in ticks from the thai-myanmar border and vietnam. | a total of 650 ticks, including 13 species from five genera, were collected from animals, from people, or by flagging of the vegetation at sites on the thai-myanmar border and in vietnam. they were tested by pcr to detect dna of bacteria of the order rickettsiales: three anaplasma spp. were detected in ticks collected in thailand, including (i) anaplasma sp. strain anda465, which was considered a genotype of anaplasma platys (formerly ehrlichia platys) and which was obtained from dermacentor aur ... | 2003 | 12682151 |
| severe japanese spotted fever successfully treated with fluoroquinolone. | a 77 years old woman who had a bite with eschar on her left arm, was admitted to emergency ward in our hospital, because of high fever, severe malaise, skin eruption, and consciousness disturbance beginning 5 days previously. she was diagnosed as japanese spotted fever by seropositive of rickettsia japonica (r. japonica) antibody, and successfully treated with fluoroquinolone, after minocycline hydrochloride had been proven ineffective. r. japonica-specific dna was detected by pcr from the tick: ... | 2006 | 17170509 |
| a trypanosome species isolated from naturally infected haemaphysalis hystricis ticks in kagoshima prefecture, japan. | common arthropod vectors for trypanosomes are flies, fleas and bugs. this study reports on an unknown trypanosome species isolated from naturally infected haemaphysalis hystricis ticks, hereby, referred to as trypanosoma kg1 isolate. the parasite has been successfully cultured in vitro with l929 or hek 293t cell line as feeder cells. this trypanosome cannot survive in vitro without feeder cells. following experimental infections of ticks, the trypomastigote-like and the epimastigote-like forms o ... | 2007 | 17306058 |
| prevalence of borrelia burgdorferi sensu lato in ticks from eastern china. | to explore the tick distribution and prevalence of borrelia in zhejiang province, we performed a survey in nine sites. a total of 447 adult ticks of 11 species were captured and the dominant tick species were haemaphysalis longicornis and ixodes sinensis and the abundance of tick species in different areas varied significantly. overall, 4.70% of the ticks were polymerase chain reaction (pcr) positive for borrelia. the average pcr positive rates were 5.19% for h. longicornis, 3.45% for amblyomma ... | 2015 | 25548382 |
| coxiella detection in ticks from wildlife and livestock in malaysia. | recent studies have shown that ticks harbor coxiella-like bacteria, which are potentially tick-specific endosymbionts. we recently described the detection of coxiella-like bacteria and possibly coxiella burnetii in ticks found from rural areas in malaysia. in the present study, we collected ticks, including haemaphysalis bispinosa, haemaphysalis hystricis, dermacentor compactus, dermacentor steini, and amblyomma sp. from wildlife and domesticated goats from four different locations in malaysia. ... | 2016 | 27763821 |
| surveillance of ticks and associated pathogens in free-ranging formosan pangolins (manis pentadactyla pentadactyla). | chinese pangolins are critically endangered insectivorous mammals distributed in several south and southeast asian countries. in recent years, there has been an increase in spread of tick-borne diseases in both humans and animals worldwide. currently, limited information is available on ticks and associated pathogens infesting pangolins. the objective of the present study was to survey ticks and associated pathogens in the formosan pangolin population in southeastern taiwan. free-ranging formosa ... | 2016 | 27426438 |
| bacterial community in haemaphysalis ticks of domesticated animals from the orang asli communities in malaysia. | ticks are vectors in the transmission of many important infectious diseases in human and animals. ticks can be readily found in the semi-forested areas such as the settlements of the indigenous people in malaysia, the orang asli. there is still minimal information available on the bacterial agents associated with ticks found in malaysia. we performed a survey of the bacterial communities associated with ticks collected from domestic animals found in two orang asli villages in malaysia. we collec ... | 2016 | 27132518 |
| ticks (acari: ixodidae) from medium-sized to large mammals in ehime prefecture, japan. | ixodid ticks were collected from medium-sized to large mammals in ehime prefecture, shikoku, japan. ten species of ticks (amblyomma testudinarium, dermacentor taiwanensis, haemaphysalis flava, h. formosensis, h. hystricis, h. longicornis, h. megaspinosa, ixodes nipponensis, i. ovatus, and i. tanuki) were collected from a total of 29 mammals comprising 11 species. haemaphysalis hystricis, a possible vector of japanese spotted fever in ehime prefecture, was collected from canis lupus familiaris (d ... | 2013 | 23242932 |
| the prevalence of anaplasma platys and a potential novel anaplasma species exceed that of ehrlichia canis in asymptomatic dogs and rhipicephalus sanguineus in taiwan. | canine anaplasmosis is regarded as an infection by anaplasma platys rather than zoonotic anaplasma phagocytophilum in subtropical areas based on the assumption that the common dog tick species is rhipicephalus sanguineus, which transmits e. canis and presumably a. platys. we investigated asymptomatic dogs and dog ticks from 16 communities in nantou county, taiwan to identify common dog tick species and to determine the prevalence of anaplasma and ehrlichia spp. of total 175 canine blood samples ... | 2017 | 28781347 |
| extensive diversity of rickettsiales bacteria in ticks from wuhan, china. | rickettsiales bacteria are important agents of (re)emerging infectious diseases, with ticks playing a key role in their evolution and transmission. we collected 1079 hard ticks belonging to five species (ixodes sinensis, rhipicephalus microplus, haemaphysalis flava, haemaphysalis hystricis and haemaphysalis longicornis) from cattle and goats in wuhan city, hubei province, china. the dominant tick species was h. longicornis (578, 53.57%), followed by r. microplus (354, 32.81%), h. hystricis (62, ... | 2017 | 28411028 |
| detection in malaysia of a borrelia sp. from haemaphysalis hystricis (ixodida: ixodidae). | spirochetes from the borrelia genus are known to cause diseases in humans, namely lyme disease and relapsing fever. these organisms are commonly transmitted to humans by arthropod vectors including ticks, mite, and lice. here, we report the molecular detection of a borrelia sp. from a haemaphysalis hystricis supino tick collected from wildlife in an orang asli settlement in selangor, malaysia. phylogenetic analyses of partial 16s rrna and flab gene sequences revealed that the borrelia sp. is clo ... | 2017 | 28874019 |
| phenotypic and genotypic identification of hard ticks of the genus haemaphysalis (acari: ixodidae) in peninsular malaysia. | morphotaxonomy based on phenotypic traits of immature hard ticks (acari: ixodidae) is a skill challenge and has prompted many inexperienced acarologists to adopt dna-based methods for identifying and discriminating the species. the aim of this study is therefore to utilize coi gene for verifying the morphological status of haemaphysalis ticks in peninsular malaysia. a total of 19 on-host ticks collected from four localities were first identified using specific illustrated taxonomic keys that lea ... | 2017 | 28409404 |
| detection of coxiella-like endosymbiont in haemaphysalis tick in thailand. | in this study, we focused on the molecular detection of coxiella-like bacteria using a pcr technique to identify coxiella 16s rrna sequences in haemaphysalis tick samples (105 adults, 8 nymph pools and 19 larval pools). seven haemaphysalis species obtained from 5 locations in thailand were evaluated in this work. coxiella endosymbionts could be detected in samples representing all 3 growth stages examined. the results also revealed that only 4 of 7 tick species were positive for coxiella-like en ... | 2015 | 25301324 |