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a novel approach, based on blsoms (batch learning self-organizing maps), to the microbiome analysis of ticks.ticks transmit a variety of viral, bacterial and protozoal pathogens, which are often zoonotic. the aim of this study was to identify diverse tick microbiomes, which may contain as-yet unidentified pathogens, using a metagenomic approach. dna prepared from bacteria/archaea-enriched fractions obtained from seven tick species, namely amblyomma testudinarium, amblyomma variegatum, haemaphysalis formosensis, haemaphysalis longicornis, ixodes ovatus, ixodes persulcatus and ixodes ricinus, was subject ...201323303373
ticks collected from wild and domestic animals and natural habitats in the republic of korea.ticks were collected from 35 animals from 5 provinces and 3 metropolitan cities during 2012. ticks also were collected by tick drag from 4 sites in gyeonggi-do (2) and jeollabuk-do (2) provinces. a total of 612 ticks belonging to 6 species and 3 genera were collected from mammals and a bird (n=573) and by tick drag (n=39). haemaphyalis longicornis (n=434) was the most commonly collected tick, followed by h. flava (158), ixodes nipponensis (11), amblyomma testudinarium (7), h. japonica (1), and h ...201425031468
phylogenetic analysis of the mitochondrial genomes and nuclear rrna genes of ticks reveals a deep phylogenetic structure within the genus haemaphysalis and further elucidates the polyphyly of the genus amblyomma with respect to amblyomma sphenodonti and amblyomma elaphense.we sequenced the entire mitochondrial genomes of 3 species of metastriate ticks: haemaphysalis formosensis, h. parva, and amblyomma cajennense. we also sequenced two thirds (ca. 9500bp) of the mitochondrial genomes of h. humerosa and h. hystricis. we used these 5 mitochondrial genome sequences together with the 13 tick mitochondrial genomes we sequenced previously and the 2 tick mitochondrial genomes sequenced by black and roehrdanz (1998), as well as the nuclear rrna genes from 84 ticks and mit ...201323602360
phylogenetic analysis of spotted fever group rickettsiae based on glta, 17-kda, and rompa genes amplified by nested pcr from ticks in japan.in order to understand the natural situation of rickettsiae in the ticks in japan, the rickettsial genes, glta gene, rompa gene, and 17-kda gene, were amplified from the ticks by nested pcr. the prevalences of rickettsial glta genes among haemaphysalis formosensis, h. longicornis, h. megaspinosa, ixodes ovatus, h. flava, h. kitaokai, and i. persulcatus were 62, 57, 24, 24, 19, 13, and 10%, respectively; 26% (186/722) being the average. the glta genes amplified from the ticks were classified into ...200314638993
detection of rickettsia japonica in haemaphysalis longicornis ticks by restriction fragment length polymorphism of pcr product.pcr was applied to the detection of rickettsia japonica, the causative agent of oriental spotted fever (osf), in ticks collected at two sites of the muroto area on shikoku island, a major area in japan where osf is endemic. primer pair rr190.70p and rr190.602n of the r. rickettsii 190-kda antigen gene sequence of regnery and others (r.l. regnery, c.l. spruill, and b.d. plikaytis, j. bacteriol. 173:1576-1589, 1991) primed the dna extracted from haemaphysalis longicornis ticks but not those extrac ...19957790445
surveillance of ticks and associated pathogens in free-ranging formosan pangolins (manis pentadactyla pentadactyla).chinese pangolins are critically endangered insectivorous mammals distributed in several south and southeast asian countries. in recent years, there has been an increase in spread of tick-borne diseases in both humans and animals worldwide. currently, limited information is available on ticks and associated pathogens infesting pangolins. the objective of the present study was to survey ticks and associated pathogens in the formosan pangolin population in southeastern taiwan. free-ranging formosa ...201627426438
epidemiological study of relapsing fever borreliae detected in haemaphysalis ticks and wild animals in the western part of japan.the genus borrelia comprises arthropod-borne bacteria, which are infectious agents in vertebrates. they are mainly transmitted by ixodid or argasid ticks. in hokkaido, japan, borrelia spp. were found in deer and haemaphysalis ticks between 2011 and 2013; however, the study was limited to a particular area. therefore, in the present study, we conducted large-scale surveillance of ticks and wild animals in the western part of the main island of japan. we collected 6,407 host-seeking ticks from two ...201728362864
ticks collected from migratory birds, including a new record of haemaphysalis formosensis, on jeju island, korea.migratory birds may disperse parasites across ecological barriers, and recent climate change may alter the pattern of ectoparasite dispersal via changed patterns of bird migration. in order to document the parasitization of migratory birds by ixodidae ticks on jeju island in korea, we examined 934 migratory birds comprising 75 species for ticks from 2010 to 2012. in total, 313 ticks were collected from 74 migratory birds across 17 avian species and identified based on morphological keys. these t ...201424141529
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