first isolation of a rickettsia closely related to japanese spotted fever pathogen from a tick in may 1993, we collected unfed ticks in anan, tokushima prefecture, shikoku district, japan, a highly endemic area of japanese spotted fever (jsf). we isolated jsf rickettsiae from these ticks by inoculating tick-emulsions into l-929 cells. of the four genera and seven species of ticks examined, only one larva of dermacentor taiwanensis sugimoto yielded a rickettsial isolate. the isolate (dt-1 strain) was found to belong to the serotype common to the causative agent of jsf. this was based on it ...19948189407
ultrastructure of spotted fever rickettsialike microorganisms observed in tissues of dermacentor taiwanensis (acari: ixodidae).tissues of midguts, malpighian tubules, salivary glands, and ovaries of female dermacentor taiwanensis sugimoto collected in an area endemic for japanese spotted fever were examined with an electron microscope, and the presence of two kinds of rickettsialike microorganisms was confirmed in japan. based on ultrastructural findings, a rod-shaped organism detected in all the tissues is a rickettsia; another, irregularly shaped organism that was found only in the ovary, is a wolbachia. a hemolymph t ...19938510117
genetic identification of rickettsiae isolated from ticks in japan.following the description in japan of japanese spotted fever, caused by rickettsia japonica, a search for the vector of this disease led to the isolation of several rickettsiae from various tick species. sixty-three rickettsial isolates were obtained from six different tick species, and six type strains were described by pcr and monoclonal antibody testing. we identified these six strains by amplification and sequencing of the genes encoding 16s rrna and citrate synthase. we confirmed that the i ...200212037083
phylogenetic analysis of spotted fever group rickettsiae isolated from ticks in japan.eight spotted fever group (sfg) rickettsiae isolated from ticks in japan were classified by phylogenetic analysis based on the nucleotide sequences of both the citrate synthase-encoding gene (glta) and 190-kda antigen-encoding gene (rompa). in the phylogenetic tree of glta, strains dt-1 and fla-1 isolated from the dermacentor taiwanensis and haemaphysalis frava ticks, respectively, were placed as rickettsia japonica, and strains io-1, io-2, io-25, im-1 and ip-2 from genus ixodes ticks were place ...200212061626
prevalence of borrelia burgdorferi sensu lato in ticks from eastern explore the tick distribution and prevalence of borrelia in zhejiang province, we performed a survey in nine sites. a total of 447 adult ticks of 11 species were captured and the dominant tick species were haemaphysalis longicornis and ixodes sinensis and the abundance of tick species in different areas varied significantly. overall, 4.70% of the ticks were polymerase chain reaction (pcr) positive for borrelia. the average pcr positive rates were 5.19% for h. longicornis, 3.45% for amblyomma ...201525548382
reinstatement of dermacentor bellulus (acari: ixodidae) as a valid species previously confused with d. taiwanensis and comparison of all parasitic of dermacentor taiwanensis sugimoto, 1935 specimens in the united states national tick collection revealed that two morphologically distinct dermacentor species were identified under this name. one of them corresponds to sugimoto's description of d. taiwanensis, while another species is identical to schulze's dermacentor bellulus (schulze, 1935). the latter species has not been considered valid by recent workers. d. bellulus is reinstated here as a valid species and all its stages ...201526335464
detection of rickettsial dna in ticks and wild boars in kyoto city, japan.the tick is a well-known vector for arthropod-borne pathogens, such as tick-borne encephalitis, lyme disease, japanese spotted fever and severe fever with thrombocytopenia syndrome. it is therefore important to know the tick population and distribution in our environment and wild animals in order to prevent tick-borne diseases. here, we report the results of tick surveillance from may to september 2011 at 14 geographical points and in 5 wild boars in kyoto city, kyoto prefecture, japan. we colle ...201425298315
ticks (acari: ixodidae) from medium-sized to large mammals in ehime prefecture, japan.ixodid ticks were collected from medium-sized to large mammals in ehime prefecture, shikoku, japan. ten species of ticks (amblyomma testudinarium, dermacentor taiwanensis, haemaphysalis flava, h. formosensis, h. hystricis, h. longicornis, h. megaspinosa, ixodes nipponensis, i. ovatus, and i. tanuki) were collected from a total of 29 mammals comprising 11 species. haemaphysalis hystricis, a possible vector of japanese spotted fever in ehime prefecture, was collected from canis lupus familiaris (d ...201323242932
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