| detection of phytoplasma by polymerase chain reaction of insect feeding medium and its use in determining vectoring ability. | a polymerase chain reaction (pcr)-based method was developed for the detection of phytoplasma in insect feeding medium (sucrose). a correlation was established between the transmissibility of flavescence dorée phytoplasma in the experimental leafhopper vector euscelidius variegatus and its detection by pcr in the insect feeding medium. however, phytoplasma were detected in the insects' bodies 3 weeks before they began to transmit. hence, pcr assays of the sucrose medium reflected phytoplasma vec ... | 2001 | 18944030 |
| novel rickettsiella bacterium in the leafhopper orosius albicinctus (hemiptera: cicadellidae). | bacteria in the genus rickettsiella (coxiellaceae), which are mainly known as arthropod pathogens, are emerging as excellent models to study transitions between mutualism and pathogenicity. the current report characterizes a novel rickettsiella found in the leafhopper orosius albicinctus (hemiptera: cicadellidae), a major vector of phytoplasma diseases in europe and asia. denaturing gradient gel electrophoresis (dgge) and pyrosequencing were used to survey the main symbionts of o. albicinctus, r ... | 2013 | 23645190 |
| synchrotron x-ray micro-computed tomography as a tool for in situ elucidation of insect bacteriomes. | obligate bacterial endosymbionts are common, influential associates of arthropods, and are often found in specific organs termed bacteriomes. three dimensional images of bacteriomes of the leafhopper orosius albicinctus (hemiptera: cicadellidae) were reconstructed from synchrotron-based x-ray micro-computed tomography (ct). results show that bilateral bacteriomes are located between the first and second abdominal tergites, are mushroom-shaped and consist two different types of tissue. fluorescen ... | 2014 | 24291672 |
| genetic diversity and vector transmission of phytoplasmas associated with sesame phyllody in iran. | during 2010-14 surveys in the major sesame growing areas of fars, yazd and isfahan provinces (iran), genetic diversity and vector transmission of phytoplasmas associated with sesame phyllody were studied. virtual rflp, phylogenetic, and dna homology analyses of partial 16s ribosomal sequences of phytoplasma strains associated with symptomatic plants revealed the presence of phytoplasmas referable to three ribosomal subgroups, 16srii-d, 16srvi-a, and 16srix-c. the same analyses using 16s rdna seq ... | 2017 | 27718042 |