| insect cell cultures in the study of attachment and pathogenicity of spiroplasmas and mycoplasmas. | the insect cell lines dm-1 (drosophila melanogaster), as-2 (aceratagallia sanguinolenta) and ac-20 (agallia constricta) were infected with spiroplasmas, mycoplasmas and acholeplasma laidlawii. in dm-1 cultures maintained at 25 degrees c in m1a medium, all strains multiplied except m. hyorhinis and the uncultivable sex-ratio organism. spiroplasma citri r8a2, s. floricola bnr-1 and obmg, s. apis pps-1 and the strains bc-3, corn stunt spiroplasma (css) and 277f produced cytopathogenic effects (cpe) ... | 1984 | 6424527 |
| the effects of dispersal and predator density on prey survival in an insect-red clover metacommunity. | trophic interactions are often studied within habitat patches, but among-patch dispersal of individuals may influence local patch dynamics. metacommunity concepts incorporate the effects of dispersal on local and community dynamics. there are few experimental tests of metacommunity theory using insects compared to those conducted in microbial microcosms. using connected experimental mesocosms, we varied the density of the leafhopper agallia constricta van duzee (homoptera: cicadellidae) and a ge ... | 2018 | 29301047 |
| diffuse kinetochores and holokinetic anaphase chromatin movement during mitosis in the hemipteran agallia constricta (leafhopper) cell line ac-20. | mitosis in the hemipteran agallia constricta (leafhopper) cell line ac-20 was examined by light microscopy of living and fixed cells. during early prometaphase the numerous small (0.30-3.0-microns) chromosomes appear as discrete units that lack a primary constriction. however, by late prometaphase the chromosomes are tightly packed at the spindle equator and are no longer clearly resolvable as individuals. when viewed from the side the metaphase chromatin appears as a 2-3-microns wide band that ... | 1990 | 2337929 |
| expression of wound tumor virus gene products in vivo and in vitro. | infection of agallia constricta vector cell monolayers with wound tumor virus results in the synthesis of 12 virus-specific polypeptides. confirmation that these polypeptides are virus encoded rather than virus induced was obtained by cell-free translation of in vitro synthesized viral mrna. in addition, transcription by purified wound tumor virus particles was coupled with translation of the resulting transcripts in a wheat embryo cell-free extract. six previously described structural polypepti ... | 1980 | 16789196 |
| adaptation of an insect cell line (agallia constricta) in a mammalian cell culture medium. | | 2017 | 4633071 |
| electron microscopy of the formation of wound tumor virus in abdominally inoculated insect vectors. | fifth-instar nymphs of agallia constricta leafhoppers were injected abdominally with extracts from root tumors confining wound tumor virus (wtv). the insects were sacrificed at predetermined intervals, their internal organs dissected, fixed, embedded, sectioned, stained, and examined in a siemens elmiskop i. sequential stages in virus development were reconstructed from consecutive samples of fatbody tissues. changes resulting from the infection were: (i) a viroplasm, i.e., an accumulation of el ... | 1967 | 16789084 |
| electron microscopy of a plant-pathogenic virus in the nervous system of its insect vector. | the central nervous system of wound tumor virus (wtv)-infected agallia constricta was studied by electron microscopy to obtain information concerning the virus distribution in the nervous system. wound tumor virions were mostly found in the cytoplasm of the ganglion cells and less frequently in the glial cells. wtv was occasionally observed in the perineurium cells, nerve axons, tracheoblasts, and lateral nerves. in the ganglion cells, virions appeared as individual isolated particles (v(1)), in ... | 1967 | 5623965 |