| polymerase chain reaction-restriction fragment-length polymorphism method to distinguish liriomyza huidobrensis from l. langei (diptera: agromyzidae) applied to three recent leafminer invasions. | a molecular method is presented for differentiating the morphologically cryptic leafminers liriomyza langei frick and l. huidobrensis (blanchard). this method requires polymerase chain reaction (pcr) amplification of a 1031-bp region of mitochondrial cytochrome oxidase dna followed by restriction fragment analysis using the restriction enzymes spei and ecorv. spel cuts the mitochondrial fragment of l. langei into two fragments, but does not cut the l. huidobrensis fragment. ecorv cuts the l. hui ... | 2001 | 11681681 |
| molecular survey for the invasive leafminer pest liriomyza huidobrensis (diptera: agromyzidae) in california uncovers only the native pest liriomyza langei. | liriomyza huidobrensis (blanchard) is a highly destructive invasive leafminer pest currently causing extensive damage to vegetable and horticultural crops around the world. liriomyza langei frick is a leafminer pest native to california that cannot currently be morphologically distinguished from l. huidobrensis. we used a dna-barcoding approach, a published pcr-rflp method, and a new multiplex pcr method to analyze 664 flies matching the morphological description of huidobrensis-langei. we found ... | 2014 | 26309286 |
| oxygen enhances phosphine toxicity for postharvest pest control. | phosphine fumigations under superatmospheric oxygen levels (oxygenated phosphine fumigations) were significantly more effective than the fumigations under the normal 20.9% atmospheric oxygen level against western flower thrips [frankliniella occidentalis (pergande)] adults and larvae, leafminer liriomyza langei frick pupae, grape mealybug [pseudococcus maritimus (ehrhorn)] eggs, and indianmeal moth [plodia interpunctella (hübner)] eggs and pupae. in 5-h fumigations with 1,000 ppm phosphine at 5 ... | 2011 | 22066172 |
| effects of vacuum and controlled atmosphere treatments on insect mortality and lettuce quality. | laboratory studies were conducted to determine the effects of vacuum and controlled atmosphere on mortality of aphids, nasonovia ribisnigri (mosley) and macrosiphum euphorbiae (thomas), and leafminer, liriomyza langei frick, and on the visual quality of iceberg lettuce at three different temperatures. vacuum at 50 mbar and controlled atmosphere with 6% co2 were effective in controlling aphids and leafminer larvae. complete control of n. ribisnigri and m. euphorbiae was achieved with vacuum treat ... | 2003 | 14503580 |
| genetic diversity and association analysis of leafminer (liriomyza langei) resistance in spinach (spinacia oleracea). | leafminer (liriomyza langei) is a major insect pest of many important agricultural crops, including spinach (spinacia oleracea). use of genetic resistance is an efficient, economic, and environment-friendly method to control this pest. the objective of this research was to conduct association analysis and identify single nucleotide polymorphism (snp) markers associated with leafminer resistance in spinach germplasm. a total of 300 usda spinach germplasm accessions were used for the association a ... | 2016 | 27490441 |