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the characterization by isoenzyme electrophoresis of leishmania isolated in the people's republic of china.seven isolates of leishmania from mainland china were characterized on the basis of their isoenzyme profiles for 10 enzymes. five isolates were from human visceral leishmaniasis patients, and four of these showed isoenzyme patterns similar to the marker strain of leishmania infantum, while one was similar to l. donovani sensu lato. one isolate was from a presumed reservoir host of human visceral leishmaniasis, the racoon dog nyctereutes procyanoides, and was isoenzymically indistinguishable from ...19846506159
species discrimination and phylogenetic inference of 17 chinese leishmania isolates based on internal transcribed spacer 1 (its1) sequences.leishmaniasis is a geographically widespread disease, caused by protozoan flagellates of the genus leishmania. this disease still remains endemic in china, especially in the west and northwest frontier regions. to date, the phylogenetic relationships among chinese leishmania isolates are still unclear, and the possible taxonomic diversity remains to be established. in this study, the its1-5.8s fragments of ten isolates collected from different foci in china were determined. to infer the phylogen ...201020617444
Isolation and detection of Leishmania species among naturally infected Rhombomis opimus, a reservoir host of zoonotic cutaneous leishmaniasis in Turkemen Sahara, North East of Iran.In Iran, three species of Leishmania have been incriminated as the causative agents of human leishmaniasis, Leishmania (L.) major, Leishmania tropica, and Leishmania infantum.Rhombomis opimus have been incriminated as a principal reservoirs of the parasitic protozoan Leishmania major, the causative agent of rural zoonotic cutaneous leishmaniasis (ZCL) in Iran. Rodents captured and examined to find Leishmania species using conventional methods including direct impression smear and microscopic obs ...201121945269
detection and molecular identification of leishmania rna virus (lrv) in iranian leishmania species.leishmania rna virus (lrv) was first detected in members of the subgenus leishmania (viannia), and later, the virulence and metastasis of the new world species were attributed to this virus. the data on the presence of lrv in old world species are confined to leishmania major and a few leishmania aethiopica isolates. the aim of this study was to survey the presence of lrv in various iranian leishmania species originating from patients and animal reservoir hosts. genomic nucleic acids were extrac ...201627604119
identification and phylogenetic relationship of iranian strains of various leishmania species isolated from cutaneous and visceral cases of leishmaniasis based on n-acetylglucosamine-1-phosphate transferase gene.the identity of iranian leishmania species has been resolved to some extent by some genetic markers. in this study, based on n-acetylglucosamine-1-phosphate transferase (nagt) gene, we further elucidated the identity and phylogeny of the prevalent species in this country. dnas of 121 isolates belonging to cutaneous leishmaniasis (cl) patients, canine visceral leishmaniasis (cvl) cases, and rhombomys opimus rodents were amplified by targeting a partial sequence of nagt gene. all the amplicons wer ...201424911282
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