| development of a recombinant protein-based enzyme-linked immunosorbent assay and its applications in field surveillance of rodent mice for presence of immunoglobulin g against orientia tsutsugamushi. | a recombinant protein containing the immunodominant conserved epitope region of the 56-kda outer membrane protein of the karp strain of orientia tsutsugamushi was purified to near homogeneity using recombinant dna techniques. the purified protein was used to immunize rabbits and produced an antibody that could recognize different strains of o. tsutsugamushi, as demonstrated both by western blotting and immunofluorescence assay. an enzyme-linked immunosorbent assay (elisa) based on this recombina ... | 2003 | 12738648 |
| [an outbreak of endemic typhus in baoshan city, yunnan province.] | objective: to understand the epidemiologic characteristics of endemic typhus in baoshan city. methods: epidemiological data were collected and characteristics were analyzed. igg antibody (ab) of rickettsia mooseri and orientia tsutsugamushi in serum of patients were tested using both weil-felix and ifa method. the rickettsia mooseri glta gene, rickettsia prowazekii glta gene, orientia tsutsugamushi 56 kda protein gene, sfgr ompa gene, ehrlichia sp. 16s rrna gene and anaplasma sp. 16s rrna gene i ... | 2011 | 21518541 |
| [study of epidemic area on tsutsugamushi disease in taizhou from 2013 to 2014]. | objective: to study the epidemiological characteristics of tsutsugamushi disease, and to confirm the existence of the disease's epidemic foci in taizhou. methods: from 2013 to 2014, dongxing town hospital and xingqiao town hospital were selected as specimen collection sites in jingjiang city. blood samples (5 ml) were collected from 40 patients with acute tsutsugamushi disease. a total of 59 rodents were captured with cage night method in the survey sites at 5, 7, 9, 10, and 11 months in 2013, f ... | 2017 | 28260341 |