| tick-borne encephalitis virus (tbev)-specific rt-pcr for characterization of natural foci of tbe and for other applications. | an effective detection system for tbev-rna sequences using a rt-pcr technique has been developed. in our system, specific oligonucleotide primers corresponding to the 5'-terminal noncoding region were successfully used to identify tbev sequences in ticks. to prove the specificity of the pcr products, southern blot hybridization with an internal digoxigenin-labelled probe was carried out. in this paper, we present some potential applications of this technique. the primers were used to identify 21 ... | 1997 | 9241807 |