identification of a unique vp4 serotype that is shared by a human rotavirus (69m strain) and an equine rotavirus (h-2 strain). | a cdna clone representing the vp4-encoding gene of human rotavirus strain 69m(vp7 serotype 8) was constructed and inserted into a baculovirus expression vector. baculovirus recombinants that expressed the 69 m vp4 protein in spodoptera frugiperda (sf9) cells were screened by immunofluorescence with hyperimmune antiserum to the 69m strain and purified by terminal dilution. the expressed vp4 was detected by coomassie blue staining of page-separated proteins. the antigenic relationships between the ... | 1996 | 8629943 |
widespread rotavirus h in commercially raised pigs, united states. | we investigated the presence in us pigs of rotavirus h (rvh), identified in pigs in japan and brazil. from 204 samples collected during 2006-2009, we identified rvh in 15% of fecal samples from 10 us states, suggesting that rvh has circulated in the united states since 2002, but probably longer. | 2014 | 24960190 |
cameroonian fruit bats harbor divergent viruses, including rotavirus h, bastroviruses, and picobirnaviruses using an alternative genetic code. | most human emerging infectious diseases originate from wildlife and bats are a major reservoir of viruses, a few of which have been highly pathogenic to humans. in some regions of cameroon, bats are hunted and eaten as a delicacy. this close proximity between human and bats provides ample opportunity for zoonotic events. to elucidate the viral diversity of cameroonian fruit bats, we collected and metagenomically screened eighty-seven fecal samples of eidolon helvum and epomophorus gambianus frui ... | 2018 | 29644096 |
distantly related rotaviruses in common shrews, germany, 2004-2014. | we screened samples from common shrews (sorex araneus) collected in germany during 2004-2014 and identified 3 genetically divergent rotaviruses. virus protein 6 sequence similarities to prototype rotaviruses were low (64.5% rotavirus a, 50.1% rotavirus c [tentative species k], 48.2% rotavirus h [tentative species l]). shrew-associated rotaviruses might have zoonotic potential. | 2019 | 31742508 |