| isolation and characterization of bacteria with antibacterial properties from nile tilapia (oreochromis niloticus). | one hundred and twenty bacterial isolates were obtained from the intestinal mucus of nile tilapia (oreochromis niloticus) and screened for antagonistic activity and adherence abilities. based on in vitro antagonism against two pathogens (streptococcus iniae and edwardsiella piscicida), five isolates were selected and identified by 16s rrna gene sequence analysis. all antagonistic isolates were affiliated to the genus bacillus, which showed inhibitory activity against s. iniae. only the isolate b ... | 2016 | 27033910 |
| histologic and molecular characterization of edwardsiella piscicida infection in largemouth bass (micropterus salmoides). | the genus edwardsiella is composed of a diverse group of facultative anaerobic, gram-negative bacteria that can produce disease in a wide variety of hosts, including birds, reptiles, mammals, and fish. our report describes the isolation and identification of edwardsiella piscicida associated with chronic mortality events in 2 separate captive largemouth bass (micropterus salmoides) populations in new york and florida. wet-mount biopsies of skin mucus, gill, kidney, and spleen from several affect ... | 2016 | 26951328 |
| first report of edwardsiellosis in cage-cultured sharpsnout sea bream, diplodus puntazzo from the mediterranean. | edwardsiella tarda, is a serious bacterial pathogen affecting a broad range of aquaculture fish species. the bacterium has also been reported as a human pathogen, however recent studies have dissociated the fish pathogenic edwardsiella from those isolated from humans by placing them in a new species, e. piscicida. here we report the first case of edwardsiellosis in cultured sharpsnout sea breams, diplodus puntazzo in greece. | 2015 | 26193880 |
| real-time polymerase chain reaction assays for the detection and quantification of edwardsiella tarda, edwardsiella piscicida, and edwardsiella piscicida-like species in catfish tissues and pond water. | researchers have proposed the adoption of 3 distinct genetic taxa among bacteria previously classified as edwardsiella tarda; namely e. tarda, e. piscicida, and a taxon presently termed e. piscicida-like. individual real-time polymerase chain reaction (qpcr) assays were developed, based on published primers, for e. tarda, e. piscicida, and e. piscicida-like sp. to provide rapid quantitative confirmatory tests for these phenotypically ambiguous bacteria. the qpcr assays were shown to be repeatabl ... | 2015 | 25613040 |
| draft genome sequences of two genetic variant strains of edwardsiella piscicida, jf1305 and rsb1309, isolated from olive flounder (paralichythys olivaceus) and red sea bream (pagrus major) cultured in japan, respectively. | edwardsiella piscicida is a new species discovered within the group of organisms traditionally classified as edwardsiella tarda. we present draft genome sequences of two variant strains of e. piscicida, jf1305 and rsb1309. differences in protein-coding sequence between these isolates are associated with virulence, disease, and defense, suggesting differences in pathogenicity. | 2014 | 24926054 |
| multilocus variable number tandem repeat analysis of edwardsiella piscicida isolates pathogenic to fish. | this study describes a novel multilocus variable number tandem repeat analysis (mlva) based on six variable number of tandem repeat (vntr) loci for genotyping of 37 edwardsiella piscicida (previously edwardsiella tarda) isolates from multiple sources. the number of alleles identified for each of the six vntr loci ranged from 3 to 5 with vntr loci 1 (di = 0.632) and 3 (di = 0.644), displaying the highest degrees of polymorphism. mlva typing of the 37 e. piscicida isolates resulted in the identifi ... | 2014 | 24749655 |
| edwardsiella piscicida-like pathogen in cultured grouper. | an edwardsiella sp. was isolated from the kidney of diseased groupers (epinephelus aeneus and e. marginatus) cultured in eilat (israel, red sea). affected fish presented a severe suppurative nephritis with large abscesses occasionally spreading into the surrounding musculature. biochemical profiles and phenotypic comparisons failed to provide a clear identification to the species level, and genetic analysis of the 16s subunit failed to discriminate between edwardsiella piscicida, e. tarda and e. ... | 2016 | 27667811 |
| complete genome sequence of an edwardsiella piscicida-like species isolated from diseased grouper in israel. | the edwardsiella piscicida-like sp. is a gram-negative facultative anaerobe that causes disease in some fish species. we report here the complete genome sequence of a virulent isolate from a diseased white grouper (epinephelus aeneus) raised on the red sea in israel, which contains a chromosome of 3,934,167 bp and no plasmids. | 2015 | 26205870 |
| edwardsiella piscicida sp. nov., a novel species pathogenic to fish. | this study describes a novel species within the genus edwardsiella based on phenotypic and genetic characterization of fish pathogenic edwardsiella isolates previously identified as e. tarda. | 2013 | 23167785 |
| genetic studies to re-affiliate edwardsiella tarda fish isolates to edwardsiella piscicida and edwardsiella anguillarum species. | until 2012, the genus edwardsiella was composed by three species edwardsiella tarda, edwardsiella hoshinae and edwardsiella ictaluri. in 2013, edwardsiella piscicida, compiling fish pathogenic strains previously identified as e. tarda was described, and more recently a new species isolated from diseased eel was reported, namely edwardsiella anguillarum. the incorporation of these species into the genus makes necessary a revision of the taxonomic position of the isolates previously identified as ... | 2017 | 29150173 |
| comparative phenotypic and genotypic analysis of edwardsiella isolates from different hosts and geographic origins, with emphasis on isolates formerly classified as e. tarda, and evaluation of diagnostic methods. | edwardsiella spp. are responsible for significant losses in important wild and cultured fish species worldwide. recent phylogenomic investigations have determined that bacteria historically classified as edwardsiella tarda actually represent three genetically distinct yet phenotypically ambiguous taxa with various degrees of pathogenicity in different hosts. previous recognition of these taxa was hampered by the lack of a distinguishing phenotypic character. commercial test panel configurations ... | 2017 | 28978684 |
| h-ns binding to evpb and evpc and repressing t6ss expression in fish pathogen edwardsiella piscicida. | edwardsiella piscicida is an important causative agent of hemorrhagic septicemia in fish and infects both cultured and wild fish species. type vi secretion system (t6ss) was proved to play important roles in pathogenesis of e. piscicida. in this study, it was demonstrated that the expression of t6ss genes evpb and evpc was under control of the global regulator h-ns in e. piscicida and the transcriptional level of evpb and evpc was significantly down-regulated by h-ns. compared to the wild type, ... | 2016 | 27125651 |
| comparative proteomic analysis unravels a role for esrb in the regulation of reactive oxygen species stress responses in edwardsiella piscicida. | as a leading pathogen, edwardsiella piscicida can cause hemorrhagic septicemia in fish and gastro-intestinal infections in humans. the two-component regulatory system esra-esrb plays essential roles in pathogenesis through the type iii and type vi secretion systems, and hemolysin production in e. piscicida it is unclear whether other virulence- or stress response-associated genes are regulated by esra-esrb. in this study, the proteomes of wild-type e. piscicida eib202 and esrb mutant strains wer ... | 2017 | 27915248 |
| identification and functional characterization of eseh, a new effector of the type iii secretion system of edwardsiella piscicida. | edwardsiella piscicida, a bacterial pathogen in fish and humans, expresses a type iii secretion system (t3ss) that is critical for pathogen virulence and disease development. however, little is known about the associated effectors and their functional importance. in this study, we identified the etae_1757 encoded protein, termed here e. piscicida secretion effector h (eseh) as a novel t3ss effector. we found that upon infection with e. piscicida, eseh is translocated into nucleus of host cells w ... | 2017 | 27367317 |
| esrb negatively regulates expression of the glutamine sythetase glna in the fish pathogen edwardsiella piscicida. | edwardsiella piscicida is a gram-negative bacterial pathogen invading a wide range of fish species. response regulator esrb is essential for the activation of type iii and type vi secretion systems (t3/t6ss). in this study, proteomes of the wild-type e. piscicida eib202 and δesrb mutant strains were compared to identify the regulon components of esrb cultured in dmem allowing t3/t6ss expression. as a result, 19 proteins showed different expression, which were identified to be associated with t3/ ... | 2018 | 29346648 |
| goldfish, carassius auratus, as an infection model for studying the pathogenesis of edwardsiella piscicida. | this study demonstrates the feasibility of using goldfish as an infection model to investigate the pathogenesis of edwardsiella piscicida. goldfish were found to be susceptible to acute e. piscicida-induced disease and died in a dose-dependent manner. e. piscicida was further shown to replicate rapidly in the head kidneys and livers of infected goldfish from 1 d post-injection, and bacteria numbers were significantly decreased 5 d post-injection. immune responses were successfully induced in gol ... | 2017 | 29119302 |
| novel t3ss effector esek in edwardsiella piscicida is chaperoned by esch and escs to express virulence. | bacterium usually utilises type iii secretion systems (t3ss) to deliver effectors directly into host cells with the aids of chaperones. hence, it is very important to identify bacterial t3ss effectors and chaperones for better understanding of host-pathogen interactions. edwardsiella piscicida is an invasive enteric bacterium, which infects a wide range of hosts from fish to human. given e. piscicida encodes a functional t3ss to promote infection, very few t3ss effectors and chaperones have been ... | 2018 | 29024267 |
| time-resolved transposon insertion sequencing reveals genome-wide fitness dynamics during infection. | transposon insertion sequencing (tis) is a powerful high-throughput genetic technique that is transforming functional genomics in prokaryotes, because it enables genome-wide mapping of the determinants of fitness. however, current approaches for analyzing tis data assume that selective pressures are constant over time and thus do not yield information regarding changes in the genetic requirements for growth in dynamic environments (e.g., during infection). here, we describe structured analysis o ... | 2017 | 28974620 |
| edwardsiella piscicida lacking the cyclic amp receptor protein (crp) is avirulent and immunogenic in fish. | edwardsiella piscicida is a gram-negative pathogen that generally causes lethal septicemia in marine and freshwater fish. we generated a e. piscicida ck216 δcrp mutant to investigate various biological roles related to this organism, including pathogenesis. lack of crp in ck216 was demonstrated by immunoblotting using a crp-specific antibody. compared to the parental strain, the mutant exhibited changes in three biochemical phenotypes, including ornithine decarboxylation, citrate utilization, an ... | 2017 | 28668485 |
| virulence regulation during late infection by a fish pathogen; sense and sensibility of bacteria may lead to novel vaccine development strategy. | | 2017 | 28641041 |
| transposon insertion sequencing reveals t4ss as the major genetic trait for conjugation transfer of multi-drug resistance peib202 from edwardsiella. | conjugation is a major type of horizontal transmission of genes that involves transfer of a plasmid into a recipient using specific conjugation machinery, which results in an extended spectrum of bacterial antibiotics resistance. however, there is inadequate knowledge about the regulator and mechanisms that control the conjugation processes, especially in an aquaculture environment where a cocktail of antibiotics may be present. here, we investigated these with peib202, a typical multi-drug resi ... | 2017 | 28499353 |
| transcriptomic dissection of the horizontally acquired response regulator esrb reveals its global regulatory roles in the physiological adaptation and activation of t3ss and the cognate effector repertoire in edwardsiella piscicida during infection toward turbot. | edwardsiella piscicida is the leading pathogen threatening worldwide aquaculture industries. the 2-component system (tcs) esra-esrb is essential for the pathogenesis of this bacterium. however, little is known about the regulon and regulatory mechanism of esra-esrb or about the factors that mediate the interaction of tcs with bacterial hosts. here, our rna-seq analysis indicated that esrb strongly induces type iii and type vi secretion systems (t3/t6ss) expression and that it modulates the expre ... | 2017 | 28441105 |
| draft genome sequence of edwardsiellapiscicida strain acc35.1 isolated from diseased turbot (scophthalmus maximus) in europe. | edwardsiella piscicida is a bacterial fish pathogen with a high degree of virulence. the strain acc35.1 was isolated from diseased turbot in europe. the draft genome sequence comprises 3.84 mb with a g+c content of 59.8% and >3,450 protein-coding genes. | 2017 | 28209828 |
| interactome of e. piscicida and grouper liver proteins reveals strategies of bacterial infection and host immune response. | the occurrence of infectious diseases is related to heterogeneous protein interactions between a host and a microbe. therefore, elucidating the host-pathogen interplay is essential. we previously revealed the protein interactome between edwardsiella piscicida and fish gill cells, and the present study identified the protein interactome between e. piscicida and e. drummondhayi liver cells. e. drummondhayi liver cells and bacterial pull-down approaches were used to identify e. piscicida outer memb ... | 2017 | 28045121 |
| complete genome sequence of edwardsiella piscicida isolate s11-285 recovered from channel catfish (ictalurus punctatus) in mississippi, usa. | edwardsiella piscicida is a recently described gram-negative facultative anaerobe and an important pathogen to many wild and cultured fish species worldwide. here, we report the complete and annotated genome of e. piscicida isolate s11-285 recovered from channel catfish (ictalurus punctatus), consisting of a chromosome of 3,923,603 bp and 1 plasmid. | 2016 | 27881536 |
| edwardsiella piscicida-associated septicaemia in a blotched fantail stingray taeniura meyeni (müeller & henle). | | 2016 | 26850591 |
| complete genome sequence of an edwardsiella piscicida-like species, recovered from tilapia in the united states. | an edwardsiella piscicida-like species is a gram-negative facultative anaerobe that causes disease in some fish species. in this report, we present the complete and annotated genome of isolate ladl05-105, recovered from cultured tilapia reared in louisiana, which contains a chromosome of 4,142,037 bp and no plasmids. | 2015 | 26337892 |
| edwardsiella piscicida identified in the southeastern usa by gyrb sequence, species-specific and repetitive sequence-mediated pcr. | a new edwardsiella taxon was recently described from fishes of europe and asia. phenotypically similar to e. tarda, extensive genetic and phenotypic characterization determined this new strain does not belong to any established edwardsiella taxa, leading to the adoption of a new taxon, e. piscicida. concurrent research in the usa also identified 2 genetically distinct taxa within the group of organisms traditionally classified as e. tarda. comparisons of gyrb sequences between us isolates and e. ... | 2014 | 24492051 |