| a leafhopper-transmissible dna virus with novel evolutionary lineage in the family geminiviridae implicated in grapevine redleaf disease by next-generation sequencing. | a graft-transmissible disease displaying red veins, red blotches and total reddening of leaves in red-berried wine grape (vitis vinifera l.) cultivars was observed in commercial vineyards. next-generation sequencing technology was used to identify etiological agent(s) associated with this emerging disease, designated as grapevine redleaf disease (grd). high quality rna extracted from leaves of grape cultivars merlot and cabernet franc with and without grd symptoms was used to prepare cdna librar ... | 2013 | 23755117 |
| association of a dna virus with grapevines affected by red blotch disease in california. | in the napa valley of california, vineyards of 'cabernet franc' (cf) clone 214, 'cabernet sauvignon' clone 337, and 'zinfandel' clone 1a (z1a) with grapevines exhibiting foliar symptoms of red blotches, marginal reddening, and red veins that were accompanied by reduced sugar accumulation in fruit at harvest were initially suspected to be infected with leafroll-associated viruses. however, reverse-transcription polymerase chain reaction (pcr) tests were negative for all known leafroll-associated ... | 2013 | 23656312 |
| sybr(®) green-based real-time quantitative reverse-transcription pcr for detection and discrimination of grapevine viruses. | a sybr(®) green-based real-time quantitative reverse transcription pcr (qrt-pcr) assay in combination with melt-curve analysis (mca) was optimized for the detection of nine grapevine viruses. the detection limits for simplex qrt-pcr for all nine grapevine viruses were estimated to be in the range of 214-1112 copies of the virus genome. amplicons with melting temperatures (tm) separated by at least 2°c in the mca could differentiate two viruses in the same reaction. therefore, eight of the nine v ... | 2016 | 27246908 |
| the complete nucleotide sequence and genomic characterization of grapevine asteroid mosaic associated virus. | in analyzing grapevine clones infected with grapevine red blotch associated virus, we identified a small number of isometric particles of approximately 30nm in diameter from an enriched fraction of leaf extract. a dominant protein of 25kda was isolated from this fraction using sds-page and was identified by mass spectrometry as belonging to grapevine asteroid mosaic associated virus (gamav). using a combination of three methods rna-seq, srna-seq, and sanger sequencing of rt- and race-pcr product ... | 2017 | 27720957 |
| spatiotemporal spread of grapevine red blotch-associated virus in a california vineyard. | grapevine red blotch-associated virus (grbav), the causative agent of red blotch disease, is a member of the genus grablovirus, in the family geminiviridae and the first known geminivirus of vitis spp. limited information is available on the epidemiology of red blotch disease. a 2-hectare vitis vinifera cv. 'cabernet franc' vineyard in napa county, california, usa was selected for monitoring grbav spread over a three-year period (2014-2016) based on an initially low disease incidence and an aggr ... | 2017 | 28392444 |
| red blotch disease alters grape berry development and metabolism by interfering with the transcriptional and hormonal regulation of ripening. | grapevine red blotch-associated virus (grbav) is a major threat to the wine industry in the usa. grbav infections (aka red blotch disease) compromise crop yield and berry chemical composition, affecting the flavor and aroma properties of must and wine. in this study, we combined genome-wide transcriptional profiling with targeted metabolite analyses and biochemical assays to characterize the impact of the disease on red-skinned berry ripening and metabolism. using naturally infected berries coll ... | 2017 | 28338755 |
| phylogeny of geminivirus coat protein sequences and digital pcr aid in identifying spissistilus festinus as a vector of grapevine red blotch-associated virus. | grapevine red blotch-associated virus (grbav) is a single-stranded dna virus, proposed to be a member of the family geminiviridae, and is associated with grapevines showing red blotch symptoms in north america. the existence of the virus was reported in 2012, and subsequently detected in grapevines in major grape production regions. we investigated if a vector exists that can transmit grbav in vineyards. phylogenetic analysis of the predicted amino acid sequence of coat protein (cp) of grbav wit ... | 2016 | 27111804 |
| grapevine red blotch-associated virus is present in free-living vitis spp. proximal to cultivated grapevines. | red blotch is an emerging disease of grapevine associated with grapevine red blotch-associated virus (grbav). the virus spreads with infected planting stocks but no vector of epidemiological significance has been conclusively identified. a vineyard block of red-blotch-affected vitis vinifera 'cabernet franc' clone 214 was observed in california, with a clustering of infected, symptomatic vines focused along one edge of the field proximal to a riparian habitat with free-living vitis spp. no genet ... | 2016 | 26960112 |
| subcellular localization of grapevine red blotch-associated virus orfs v2 and v3. | grapevine red blotch-associated virus is a recently discovered plant monopartite gemini-like virus found in north american grapevines. leaf discoloration and a decrease in fruit quality are associated with its infection. two of its six open reading frames (orfs), v2 and v3, are of unknown function and share no obvious homology with plant or viral genes. transient expression of these orfs in fusion with the green fluorescent protein demonstrated that the v2 protein localizes in the nucleoplasm, c ... | 2015 | 26063598 |
| grapevine red blotch-associated virus, an emerging threat to the grapevine industry. | grapevine red blotch-associated virus (grbav) is a newly identified virus of grapevines and a putative member of a new genus within the family geminiviridae. this virus is associated with red blotch disease that was first reported in california in 2008. it affects the profitability of vineyards by substantially reducing fruit quality and ripening. in red-berried grapevine cultivars, foliar disease symptoms consist of red blotches early in the season that can expand and coalesce across most of th ... | 2015 | 25738551 |
| grapevine red blotch-associated virus is widespread in the united states. | grapevine red blotch disease has been recognized since 2008 as affecting north american grape production. the presence of the newly described grapevine red blotch-associated virus (grbav) is highly correlated with the disease. to more effectively detect and monitor the presence of the virus, a sample processing strategy and multiplex polymerase chain reaction assay were developed. a total of 42 of 113 vine samples collected in or received from seven of the united states were shown to harbor the ... | 2014 | 24805072 |