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[identification and characterization of a flavonoid-3-o-glucosyltransferase gene from saussurea involucrata].the flavonoid-3-o-glucosyltransferas (3gt) is one of the most important enzymes for biosynthesis of plant secondary metabolites. in this paper, the homology analysis and gene special primers design were used. with the methods of modern molecular biology, the full-length gene of 3gt (genbank accession no. jn092127) which was cloned from saussurea involucrata kar. et kir by rt-pcr and race. the cdna sequence of 3gt consisted of 1548 bp open reading frame (orf) encoding 516 amino acid, the deduced ...201223016307
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