| biotechnological approaches to determine the impact of viruses in the energy crop plant jatropha curcas. | abstract: background: geminiviruses infect a wide range of plant species including jatropha and cassava both belonging to family euphorbiaceae. cassava is traditionally an important food crop in sub - saharan countries, while jatropha is considered as valuable biofuel plant with great perspectives in the future. results: a total of 127 jatropha samples from ethiopia and kenya and 124 cassava samples from kenya were tested by enzyme-linked immunosorbent assay (elisa) for rna virus and polymerase ... | 2011 | 21812981 |
| quantification of african cassava mosaic virus (acmv) and east african cassava mosaic virus (eacmv-ug) in single and mixed infected cassava (manihot esculenta crantz) using quantitative pcr. | the quantity of genomic dna-a and dna-b of african cassava mosaic virus (acmv) and east african cassava mosaic virus uganda (uganda variant, eacmv-ug) was analysed using quantitative pcr to assess virus concentrations in plants from susceptible and tolerant cultivars. the concentrations of genome components in absolute and relative quantification experiments in single and mixed viral infections were determined. virus concentration was much higher in symptomatic leaf tissues compared to non-sympt ... | 2016 | 26456453 |
| virus versus host plant micrornas: who determines the outcome of the interaction? | considering the importance of micrornas (mirnas) in the regulation of essential processes in plant pathogen interactions, it is not surprising that, while plant mirna sequences counteract viral attack via antiviral rna silencing, viruses in turn have developed antihost defense mechanisms blocking these rna silencing pathways and establish a counter-defense. in the current study, computational and stem-loop reverse transcription - polymerase chain reaction (rt-pcr) approaches were employed to a) ... | 2014 | 24896088 |
| spatial analysis of temporal changes in the pandemic of severe cassava mosaic disease in north-western tanzania. | to improve understanding of the dynamics of the cassava mosaic disease (cmd) pandemic front, geospatial approaches were applied to the analysis of three years' data obtained from a 2° by 2° (ca 222km by 222 km) area of north-western tanzania. a total of 80 farmers' fields were assessed in each of 2009, 2010 and 2011, with 20 evenly-distributed fields per 1° by 1° quadrant. cmd-associated variables (cmd incidence, cmd severity, vector-borne cmd infection and vector abundance) increased in magnitu ... | 2017 | 28714353 |
| fitness costs associated with infections of secondary endosymbionts in the cassava whitefly species bemisia tabaci. | we investigated the dual effects of bacterial infections and diseased cassava plants on the fitness and biology of the bemisia tabaci infesting cassava in africa. isofemale b. tabaci colonies of sub-saharan africa 1-subgroup 3 (ssa1-sg3), infected with two secondary endosymbiotic bacteria arsenophonus and rickettsia (ar+) and those free of ar infections (ar-), were compared for fitness parameters on healthy and east african cassava mosaic virus-uganda variant (eacmv-ug)-infected cassava plants. ... | 2018 | 29367840 |