capulavirus and grablovirus: two new genera in the family geminiviridae. | geminiviruses are plant-infecting single-stranded dna viruses that occur in most parts of the world. currently, there are seven genera within the family geminiviridae (becurtovirus, begomovirus, curtovirus, eragrovirus, mastrevirus, topocuvirus and turncurtovirus). the rate of discovery of new geminiviruses has increased significantly over the last decade as a result of new molecular tools and approaches (rolling-circle amplification and deep sequencing) that allow for high-throughput workflows. ... | 2017 | 28213872 |
identification and molecular characterization of a novel monopartite geminivirus associated with mulberry mosaic dwarf disease. | high-throughput sequencing of small rnas allowed the identification of a novel dna virus in a chinese mulberry tree affected by a disease showing mosaic and dwarfing symptoms. rolling-circle amplification and pcr with specific primers, followed by sequencing of eleven independent full-length clones, showed that this virus has a monopartite circular dna genome (∼ 2.95 kb) containing orfs in both polarity strands, as reported previously for geminiviruses. a field survey showed the close associatio ... | 2015 | 25953916 |