| sequence-modified primers for the differential rt-pcr detection of andean potato latent and andean potato mild mosaic viruses in quarantine tests. | to enable the differential pcr detection of andean potato latent virus (aplv) and andean potato mild mosaic virus (apmmv) strains, sense primers were designed that correspond to regions directly upstream of the coat protein genes. their differentiating power was increased by a->c or t->c replacements in their 3'-terminal parts. together with the broad-specificity antisense primer em3, primer al-a-mod3c detected all aplv strains tested, but none of the apmmv strains. primer am-a-mod4c yielded pcr ... | 2014 | 24221248 |
| the complete genome sequences of a peruvian and a colombian isolate of andean potato latent virus and partial sequences of further isolates suggest the existence of two distinct potato-infecting tymovirus species. | the complete genomic rna sequences of the tymovirus isolates hu and col from potato which originally had been considered to be strains of the same virus species, i.e. andean potato latent virus (aplv), were determined by sirna sequencing and assembly, and found to share only c. 65% nt sequence identity. this result together with those of serological tests and comparisons of the coat protein gene sequences of additional tymovirus isolates from potato suggest that the species andean potato latent ... | 2013 | 23357297 |