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a real-time pcr assay for bat sars-like coronavirus detection and its application to italian greater horseshoe bat faecal sample surveys.bats are source of coronaviruses closely related to the severe acute respiratory syndrome (sars) virus. numerous studies have been carried out to identify new bat viruses related to sars-coronavirus (bat-sars-like covs) using a reverse-transcribed-polymerase chain reaction assay. however, a qualitative pcr could underestimate the prevalence of infection, affecting the epidemiological evaluation of bats in viral ecology. in this work an sybr green-real time pcr assay was developed for diagnosing ...201122654650
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